棕叶花提取物对动物离体子宫的兴奋作用

棕叶花提取物对动物离体子宫的兴奋作用作者：唐祖年，梁成钦，覃文科，李丽

【摘要】目的探讨棕叶花不同提取物对家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用。方法采用BL-410生物机能实验系统，观察棕叶花4种提取物对非孕或注射己烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用，并用EC50与益母草流浸膏进行比较。结果棕叶花正丁醇组分、水溶性组分均能使非孕或注射己烯雌酚后大鼠、小鼠、豚鼠、家兔离体子宫平滑肌的收缩频率增快、收缩幅度增强、张力明显提高，其兴奋子宫的强度比益母草流浸膏显著（P<0.05）。而石油醚组分、醋酸乙酯组分对上述动物兴奋子宫作用微弱。结论棕叶花正丁醇、水溶性组分对家兔、豚鼠、大鼠、小鼠离体子宫有明显兴奋作用。对离体子宫兴奋作用比益母草流浸膏显著。

【关键词】棕叶花；离体子宫；兴奋作用

Abstract:ObjectiveTo study the effects of different extracts of Phrynium capitatum Willd flower on the uterus of rabbit, guinea pig, rat and mouse in vitro. MethodsIsolated uterus preparations of above animals were made according to the literature and its contractive effects to four different extracts of Phrynium capitatum Willd flower were observed and

recorded with BL-410 experimental system for biological function; the EC50s of the extracts were determined and compared with it of Herba Leonuri fluid extract. Results It was found that the extracts with butyl alcohol and with water could increase both contraction frequency and force of smooth muscle of the uterus of non-pregnant or diethylstilbestrol-injected animals, which were much potent than that induced by Herba Leonuri fluid extract. (P<0.05). However, the effect of the extract with petroleum ether and with ethyl cyanocaetate was negligible. ConclusionThe extracts of Phrynium capitatum Willd flower with butyl alcohol and with water have excitatory effects on the non-pregnant or diethylstilbestrol-injected animal uterus in vitro ,which is more potent than that of Herba Leonuri fluid extract.

Key words:Phrynium capitatum Willd flower; Isolated uterus; Excitatory effect

棕叶花（别名冬叶花、柊叶花）Phrynium capitatum Willd (flower)为竹芋科（Marantaceae）多年生本草，分布广西、广东、云南等地。全草可入药。文献记载：“全草味甘淡，性微寒，有清热解毒、凉血、止血和利尿之功效。”广西少数民族民间有用其花治疗月经过多、

血崩（功能性子宫出血）及失音等疾病之经验（《广西民族药简编》，1980广西药品检验所印行）。有文章报道棕叶花水提取物对孕或非孕小鼠、大鼠、豚鼠、家兔离体子宫及对家兔在体子宫都有兴奋作用［1］，而关于其他溶剂提取物的药理研究尚未见报道。为了进一步研究其他溶剂提取物对子宫的兴奋作用，本文采用BL-410生物机能实验系统，观察棕叶花正丁醇、水溶性组分两种提取液对非孕或注射乙烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的影响，并用EC50与益母草流浸膏进行比较。

1 器材与方法

1.1 受试药物与试剂棕叶花采自南宁地区，晒干备用。受试药物的制备：取干棕叶花750 g用中药材粉碎机粉碎后置10 000 ml 圆底烧瓶中加95%乙醇浸没，加热冷凝回流1 h，抽滤，重复上述操作3次；药渣50%乙醇浸没，热冷凝回流1 h，抽滤，重复3次。合并滤液，用旋转蒸发仪浓缩。再加适量蒸馏水使浓缩物完全溶解，置6 000 ml的锥形瓶中，加石油醚1 000 ml振摇1 h，然后离心15 min（6 000 r/min），取上层液体，如此重复5次；剩下部分用醋酸乙酯、正丁醇、蒸馏水重复石油醚操作。所得的石油醚（为1号）、醋酸乙酯（为2号）、正丁醇（为3号）、水溶性组分（为6号）旋转蒸发仪浓缩，45℃烘干后置冰箱备用。益母草流浸膏经烤箱60℃烘干，固体经磨粹后放入磨口玻璃瓶备用。