线虫实验11ppt课件
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1 cell at fertilization to 959/1031 cells in adult
temperature-dependent
15℃,5.5day; 20℃,3.5day; 25℃,2.5day
11
线虫的发育
12
线虫的发育
13
1-dimensional genetic information
100,281,416 bp ~20,000 genes
….GGTATCCATTAGGTAT……
C. elegans work is desirable. Interested applicants should send
a CV and the names and contact information of 3 persons who can provide a letters of reference. Please send information by email to okv2@cornell.edu.
The two touch receptor neurons ALMR and PLMR are fluorescencelabelled at their cell bodies, leading to the strongly fluorescing two dots in the figure. Fluorescing processes can be seen projecting from both of these cell bodies. Halos produced from the out-of-focus of homologues of these cells on the other side of the animal are indicated by arrow heads. The thick arrow points to the nerve ring branch from the ALMR cell (out of focus); the thin arrows point to weakly fluorescing cell bodies.
9
Βιβλιοθήκη Baidu
线虫的生物学
秀丽隐杆线虫有5对常染色体和1对性染色体；性别为 雌雄同体或雄性（自然群体中为千分之一）；
杂交时，后代雌雄比例为1：1 成虫一生可产300个受精卵； 有大量突变株； 可以用-80℃冰箱长期保存 线虫基因组测序1998年完成
10
Life cycle of C. elegans
7
实验原理
线虫的生物学
8
线虫的生物学
通常所说的线虫特指秀丽隐杆线虫(Caenorhabditis elegans)是一种很小的蠕虫。
秀丽隐杆线虫成体长仅1mm，全身透明，以细菌为食， 居住在土壤中，全身共有959个细胞。
线虫整个的生命周期仅3天（25℃）。野生型线虫胚 胎发育中细胞分裂和细胞系的形成具有高度的程序性， 这样就便于对其发育进行遗传学分析。
2
2002诺贝尔奖
Brenner, Horvitz & Sulston
“For their discoveries concerning genetic regulation of organ development and programmed cell death.”
3
2006诺贝尔奖
"for their discovery of RNA interference - gene silencing by doublestranded RNA"
6
Postdoctoral position, Vatamaniuk Lab, Cornell
A postdoctoral position is available in a young research group at Cornell University to study genetic and biochemical interactions of ATP-bindings cassette (ABC) transporters and the molecular mechanisms of their function in detoxification of heavy metals. We seek highly motivated, responsible, and talented individuals with extensive experience in molecular genetics and at least some basic experience in protein biochemistry. Experience with
4
2008诺贝尔奖
"for the discovery and development of the green fluorescent protein, GFP"
Martin Chalfie Columbia University New York, USA
5
Expression of GFP from a first stage C. elegans larva (Chalfie et al., 1994).
Andrew Z. Fire Craig C. Mello
Fire A, et al. Potent and specific genetic interference by double-strand RNA in Caenorhabditis elegans. Nature, 1998,391:806-811
线虫实验
1
开设线虫有关实验的目的意义
线虫作为模式生物，与酵母、果蝇、斑马鱼、 小鼠和拟南芥等一起，为生命科学的发展做出 了极大的贡献。
目前，国内外众多的科研机构都在以线虫作为 研究材料，在RNAi、衰老、药物筛选、功能 基因组学等领域进行研究
北京生命科学研究所 、遗传与发育生物学研 究所、北京大学 、军事医学科学院、 东南大 学等单位
1 cell at fertilization to 959/1031 cells in adult
temperature-dependent
15℃,5.5day; 20℃,3.5day; 25℃,2.5day
11
线虫的发育
12
线虫的发育
13
1-dimensional genetic information
100,281,416 bp ~20,000 genes
….GGTATCCATTAGGTAT……
C. elegans work is desirable. Interested applicants should send
a CV and the names and contact information of 3 persons who can provide a letters of reference. Please send information by email to okv2@cornell.edu.
The two touch receptor neurons ALMR and PLMR are fluorescencelabelled at their cell bodies, leading to the strongly fluorescing two dots in the figure. Fluorescing processes can be seen projecting from both of these cell bodies. Halos produced from the out-of-focus of homologues of these cells on the other side of the animal are indicated by arrow heads. The thick arrow points to the nerve ring branch from the ALMR cell (out of focus); the thin arrows point to weakly fluorescing cell bodies.
9
Βιβλιοθήκη Baidu
线虫的生物学
秀丽隐杆线虫有5对常染色体和1对性染色体；性别为 雌雄同体或雄性（自然群体中为千分之一）；
杂交时，后代雌雄比例为1：1 成虫一生可产300个受精卵； 有大量突变株； 可以用-80℃冰箱长期保存 线虫基因组测序1998年完成
10
Life cycle of C. elegans
7
实验原理
线虫的生物学
8
线虫的生物学
通常所说的线虫特指秀丽隐杆线虫(Caenorhabditis elegans)是一种很小的蠕虫。
秀丽隐杆线虫成体长仅1mm，全身透明，以细菌为食， 居住在土壤中，全身共有959个细胞。
线虫整个的生命周期仅3天（25℃）。野生型线虫胚 胎发育中细胞分裂和细胞系的形成具有高度的程序性， 这样就便于对其发育进行遗传学分析。
2
2002诺贝尔奖
Brenner, Horvitz & Sulston
“For their discoveries concerning genetic regulation of organ development and programmed cell death.”
3
2006诺贝尔奖
"for their discovery of RNA interference - gene silencing by doublestranded RNA"
6
Postdoctoral position, Vatamaniuk Lab, Cornell
A postdoctoral position is available in a young research group at Cornell University to study genetic and biochemical interactions of ATP-bindings cassette (ABC) transporters and the molecular mechanisms of their function in detoxification of heavy metals. We seek highly motivated, responsible, and talented individuals with extensive experience in molecular genetics and at least some basic experience in protein biochemistry. Experience with
4
2008诺贝尔奖
"for the discovery and development of the green fluorescent protein, GFP"
Martin Chalfie Columbia University New York, USA
5
Expression of GFP from a first stage C. elegans larva (Chalfie et al., 1994).
Andrew Z. Fire Craig C. Mello
Fire A, et al. Potent and specific genetic interference by double-strand RNA in Caenorhabditis elegans. Nature, 1998,391:806-811
线虫实验
1
开设线虫有关实验的目的意义
线虫作为模式生物，与酵母、果蝇、斑马鱼、 小鼠和拟南芥等一起，为生命科学的发展做出 了极大的贡献。
目前，国内外众多的科研机构都在以线虫作为 研究材料，在RNAi、衰老、药物筛选、功能 基因组学等领域进行研究
北京生命科学研究所 、遗传与发育生物学研 究所、北京大学 、军事医学科学院、 东南大 学等单位