takara宝生物公司限制酶双酶切buffer表

Note:

1) It is confirmed that 10 units of each enzyme completely digest 1 µg of DNA at 37°C in one hour in 50 µl reaction mixture.

2) The concentration of Glycerol should be less than 10% to minimize star activity.

3) DNA may not be digested completely, when recognition sequences of two enzymes are close each other, or when DNA takes high-structure conformation.

反应体系（20ul）：

10* M Buffer 2ul

质粒T-5.3kb Gt13ul XbaⅠ、HindⅢ各0.5ul（7.5U）无菌水14ul 37℃水浴保温2h