手性柱说明

This data sheet contains impaortant information about the product.

Operating instructions for

Astec CHIROBIOTIC® LC Strationary Phases

Column Installation 柱安装

CHIROBIOTIC columns are shipped in methanol . Before starting to use a new column , wash with 20ml HPLC grade methanol at 1ml/min.The column test standarad, 5-methyl-5- phenylhydantoin, can be injected at this stage to establish performance .

CHIROBIOTIC色谱柱用甲醇装运。开始使用一个新的色谱柱之前，用色谱纯甲醇以1ml/min 的流速洗涤20毫升。色谱柱测试标准，5-甲基-5- phenylhydantoin在这个阶段被注入确立表现。

New columns can take longer to equilibrate, but once baseline stability is achieved it is consistent. New threaded hardware improves column performance. Do not overtighten. This threaded hardware also allows for direct connection to a guard column.

新柱子需要更长的时间达到平衡，但一旦基线达到稳定,是一致的。新的螺纹硬件提高柱子的性能。不要拧得过紧。该螺纹硬件也可直接连接预柱。

General Operating Conditions 常见的操作条件

Flow Rate Range: 0.2 to 3.0 mL/min Typically-1.0mL/min Pressure Range: Up to 3500 psi (240 bar) See details below

pH Range: CHIROBIOTIC V and V2 2.7 to 7

CHIROBIOTIC T and T2 2.7 to 7

CHIROBIOTIC R 2.7 to 7

CHIROBIOTIC TAG 2.7 to 7 Injection Volumn: 1 to 5μL of a 1mg/mL solution See details below

Mobile Phases 流动相

All known HPLC solvents and buffers are compatible with CHIROBIOTIC phases. The only critical operating parameter detrimental to these columns is extremes of pH. For best performance we highly recommend the Supelco Mobile Phase Degasser (Cat No.55018-U).

所有已知的HPLC溶剂和缓冲溶液与CHIROBIOTIC相兼容。唯一对这些色谱柱有损害的关键运行参数是极端的pH。为了获得最佳性能，我们强烈建议使用Supelco公司流动相脱气机。

Sample Solvents样品溶剂

Expect best results when mobile phase is used as the sample solvent. When the sample solvent has stronger eluotropic properties than the mobile phase, components of the sample may deposit on the head of the column causing poor peak shape and reduced column lifetime. Even if sample adsorption does not occur, incompatibilities between the sample solvent and mobile phase may cause peak distortion, especially in early-eluting compounds.

流动相被用作样品溶剂是被期望的最佳效果。当样品溶剂具有比流动相更强洗脱特性，样品的成分会沉积在柱的头部导致峰形变差和降低柱寿命。即使样品吸附不发生，样品溶剂和流动相之间的不兼容性可能导致峰变形，尤其是在化合物早期洗脱。

Injection Volumn and Concentrations进样量和浓度

Injection volumn of 1-5μL at concentrations of 1mg/ml are desirable for good peak efficiency. The load volume and concentration may be 5 to 10 times higher without affecting resolution. Therefore, begin the separation study at the lowest volumes and concentrations until a proper determination can be made of its effect.

对1-5μl在1mg的浓度进样量/毫升是可取的好峰值效率。的负载量和浓度可以是5至10倍，而不会影响分辨率。因此，在最低体积和浓度的开始分离研究，直至一个可由其效果适当的判定的量。

Astec columns may be operated from either direction without loss of performance due to the unique packing system that produces uniform packing denisty. This is true for all Astec columns. Astec色谱柱由于独特的包装系统生产的柱子填料密度均匀，可以由任一方向操作不会损失性能。这是适用于所有Astec公司色谱柱。

Temperature 温度

Several temperature effects have been noted with the CHIROBIOTIC phases. At lower temperatures, resolution has been observed to increase with certain compound structures. It is not a generally expected phenomenon, however. No deleterious effects on column performance or stability has been observed when operating at column temperatures as 0℃. It is possible, however, to prevent racemization from occurring when operating below room temperature, as has observed with diazepines.

几个温度已经被注意到影响CHIROBIOTIC相。在较低温度下，观察到某些结构的化合物的检测分辨率增加。然而它不是通常预期的现象。已经观察在柱温为0℃工作时，对柱性能或稳定性无不利影响。它是可能的，但是，如已与二氮杂草观察到的，防止工作在低于室温时，外消旋化的发生。

At elevated temperatures two effects have been observed. First, an increase in efficiency can be obtained as with standard chromatography. Second, and most importantly, a reversal of elution order might occur at elevated temperatures. This phenomenon is again, compound dependent and not predictable. The maximum temperature that can be safely employed is 50℃in all solvent modes. Temperature changes should be made no faster than 2℃ per minutes.

在升高温度观察到两方面的影响。第一，标准色谱法能够增加效率。第二，也是最重要的是，可能发生在高温下的逆转洗脱顺序。这种现象是可以重现的，依赖化合物性质和不可预测的。所有的溶剂模式来说，可以安全使用的最高温度为50℃。温度变化不应高于每分钟2℃。

Pressure 柱压

Operating pressure for CHIROBIOTIC columns is generally in the range of 1200 to 1500 psi(80-110 bar) (150×4.6mm) and 2000 to 2500 psi (135-170 bar)(250×4.6mm) at 1.0ml/min for 20/80;MeOH/buffer. As with standard reserved phase columns, the higher the water content, the higher the back pressure, with a maxmium at 50/50 ;MeOH/H2O. Care should always be exercised in prefiltering and degassing the water and solvent used with these columns. In general, pressures should not exceed 3500 psi (240bar). Columns are packed in excess of 100000 psi,(680bar ), so column beds are extremely stable to pressure.

对于CHIROBIOTIC色谱柱在1.0毫升/分钟流速甲醇/缓冲溶液比例为20/80条件下操作压力一般在1200至1500 psi（80-110bar）（150×4.6mm）和2000〜2500 psi（135-170 bar）（250×4.6mm）。和标准的保留相色谱柱一样，含更高的水量，就有更高的压力，最大的压力在