微波辅助法提取金银花绿原酸实验流程

微波辅助法提取金银花绿原酸实验流程

1.取适量金银花粉碎，加入10倍量乙醇浸提24小时。

Take a proper amount of honeysuckle and add 10 times the amount of ethanol for extraction for 24 hours.

2.将提取液浓缩至浸膏。

Concentrate the extract to obtain the paste.

3.称取适量浸膏加入适量水溶解。

Weigh an appropriate amount of paste and add an appropriate amount of water to dissolve.

4.获得金银花提取液。

Obtain the honeysuckle extract.

5.称取一定量提取液，加入适量醋酸乙酯，加热回流30分钟。

Weigh a certain amount of extract, add an appropriate amount of ethyl acetate, and reflux for 30 minutes.

6.过滤，取滤液。

Filter and collect the filtrate.

7.浓缩滤液至干燥，得到金银花绿原酸提取物。

Concentrate the filtrate to dryness to obtain the honeysuckle chlorogenic acid extract.

8.称取一定量金银花绿原酸提取物，加入适量乙醇，超声处理。

Weigh a certain amount of honeysuckle chlorogenic acid extract, add an appropriate amount of ethanol, and perform ultrasonic treatment.

9.微波辅助法提取金银花绿原酸实验流程完毕。

The experimental process of microwave-assisted extraction of honeysuckle chlorogenic acid is completed.