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IL-4R 825 aa
Ig
FN
CCCC
III
FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III
IL-13R2 380 aa
c 349 aa
Ig
FN
CCCC
III
FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III
gp130 896 aa
Ig
FN
CCCC
III FN
WS-WS
III FN III FN III FN III
cAMP cAMP
cAMP cAMP
cAMP
cAMP
cAMP
cAMP cAMP
cAMP
PKA
14-3-3 P
RGS
14-3-3
.
2
Modular Structure of Cytokine Receptors
IFNR1 530 aa
IL-10R1 557 aa
FN
III
IFNR2 515 aa
FN
III
FN
WS-WS
III FN
III
FN
III
FN
III
LIF-R 1053 aa
FN
CCCC
III FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III FN
III
FN
III
FN
III
Tyk Jak
2
1
STAT
binding
Jak Tyk
1
2
Jak Jak
Jak
1
2
1
Jak
Tyk
Jak
1
276
SH3
SH2
1
SH2
SH3
304
SH3
1
SH3
SH2
330
SH3
1
SH3
SH3
SH3
377
SH2
1
Sprouty
456
SH2
1 SAM
533
SH2
1
PTB
584
SH2
1
632
PH
SH2
1 Calponin
Rho-GEF
PH
C-1
SH3
SH2
GRB2
SLAP
Crk
GADS (GRID)
Nck1
OSM-R 952 aa
IL-12 662 aa
IL-6R 449 aa
IL-11R 400 aa
FN
CCCC
III
FN
WS-WS
III
FN
Ig Ig III
FN
CCCC
III
FN FN
CCCC
III III
FN FN FN
WS-WS WS-WS
III
III III
FN
WS-WS
III
Ig
FN
CCCC
NO – mediated attenuation of adrenergic Gq signaling in VSMC
ANP
K+ BKCa
pGC
sGCCa
cGMP
PKG
PLC
q
GTP
q
GTP
PA
1,4,5-Ins
PKC
q
GDP
Ca Ca
Ca Ca Ca
Ca
Ca Ca Ca
Ca Ca Ca
Ca Ca Ca Ca
1
2
3
IRS
SOCS
STAT
binding
STAT
binding
FN Fibronectin III type III domain
FN Fibronectin
CCCC type III domain
III with cysteines
STAT
binding
FN Fibronectin
WS-WS type III domain
Rho-GEF homology domain
. Sprouty
Sprouty domain
PTB
PTB domain
PH
4 Plekstrin homology
domain
Protein separation using SDS-PAGE
(Laemmli system)
1. Apply protein/dye samples into polyacrylamide gel wells
2. Run the electrophoresis until dye reaches the end of the gel
Stacking gel
Resolving gel
3. Remove the gel from the apparatus and stain for proteins
.
5
A Column Chromatography Toolkit
III with WSXWS
IRS IRS-binding SOCS SOCS-binding
STAT
binding
STAT
binding
STAT
binding
SHP
STAT STAT-binding
binding
Jak 2
. Tyk 2
JAK/TYK -binding
Jak 1
SOCS SHP
源自文库STAT
further utilization
Potential use for
therapeutic cloning .
Use for In Vitro fertilization
Freeze in N2 for
long-term sto7rage
IL-10R2 325 aa
III
FN FN
FN FN
III III
III III
FN FN
FN FN
III III
III III
INFR1 489 aa
IL-22R1 574 aa
INFR2 332 aa
FN FN
FN
III III
III
FN FN
FN
III III
III
IL-13R1 427 aa
.
6
Embryonic stem cells
Sperm donor Egg donor
Egg fertilization
Culture fertilized cells for several cell divisions
Dissociate cell cluster for subculturing and
Ca
Ca Ca Ca
Ca
Ca
Ca CaM
Ca Ca
Ca Ca Ca
Ca
MLCK
Ca
Ca
Ca
.
Ca
CaM
Ca
P MLCK
P
myosin
myosin
P
myosin
1
RGS function and regulation
Ligand
S
GDP RGS
TNF
Ligand
S
GTP
RGS
P
RGS
S
GTP AC AC
binding
STAT
binding
Jak
Jak
3
3
STAT
binding
STAT
binding
Jak 3
STAT
binding
STAT
binding
SHP SHP-binding
3
Ig Ig - like
Adaptor proteins - selected (human)
1
SH3
SH2
217
SH3
BLNK
SLP-76
SHC-1
APS
845
SH3
Vav-1
SH3
Src-homology 3 domain
SH2
Src-homology 2 domain
SAM
C-1
SAM domain
Cysteine-rich region 1
Calponin
Calponin homology domain
Rho-GEF
Ig
FN
CCCC
III
FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III
IL-13R2 380 aa
c 349 aa
Ig
FN
CCCC
III
FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III
gp130 896 aa
Ig
FN
CCCC
III FN
WS-WS
III FN III FN III FN III
cAMP cAMP
cAMP cAMP
cAMP
cAMP
cAMP
cAMP cAMP
cAMP
PKA
14-3-3 P
RGS
14-3-3
.
2
Modular Structure of Cytokine Receptors
IFNR1 530 aa
IL-10R1 557 aa
FN
III
IFNR2 515 aa
FN
III
FN
WS-WS
III FN
III
FN
III
FN
III
LIF-R 1053 aa
FN
CCCC
III FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III FN
III
FN
III
FN
III
Tyk Jak
2
1
STAT
binding
Jak Tyk
1
2
Jak Jak
Jak
1
2
1
Jak
Tyk
Jak
1
276
SH3
SH2
1
SH2
SH3
304
SH3
1
SH3
SH2
330
SH3
1
SH3
SH3
SH3
377
SH2
1
Sprouty
456
SH2
1 SAM
533
SH2
1
PTB
584
SH2
1
632
PH
SH2
1 Calponin
Rho-GEF
PH
C-1
SH3
SH2
GRB2
SLAP
Crk
GADS (GRID)
Nck1
OSM-R 952 aa
IL-12 662 aa
IL-6R 449 aa
IL-11R 400 aa
FN
CCCC
III
FN
WS-WS
III
FN
Ig Ig III
FN
CCCC
III
FN FN
CCCC
III III
FN FN FN
WS-WS WS-WS
III
III III
FN
WS-WS
III
Ig
FN
CCCC
NO – mediated attenuation of adrenergic Gq signaling in VSMC
ANP
K+ BKCa
pGC
sGCCa
cGMP
PKG
PLC
q
GTP
q
GTP
PA
1,4,5-Ins
PKC
q
GDP
Ca Ca
Ca Ca Ca
Ca
Ca Ca Ca
Ca Ca Ca
Ca Ca Ca Ca
1
2
3
IRS
SOCS
STAT
binding
STAT
binding
FN Fibronectin III type III domain
FN Fibronectin
CCCC type III domain
III with cysteines
STAT
binding
FN Fibronectin
WS-WS type III domain
Rho-GEF homology domain
. Sprouty
Sprouty domain
PTB
PTB domain
PH
4 Plekstrin homology
domain
Protein separation using SDS-PAGE
(Laemmli system)
1. Apply protein/dye samples into polyacrylamide gel wells
2. Run the electrophoresis until dye reaches the end of the gel
Stacking gel
Resolving gel
3. Remove the gel from the apparatus and stain for proteins
.
5
A Column Chromatography Toolkit
III with WSXWS
IRS IRS-binding SOCS SOCS-binding
STAT
binding
STAT
binding
STAT
binding
SHP
STAT STAT-binding
binding
Jak 2
. Tyk 2
JAK/TYK -binding
Jak 1
SOCS SHP
源自文库STAT
further utilization
Potential use for
therapeutic cloning .
Use for In Vitro fertilization
Freeze in N2 for
long-term sto7rage
IL-10R2 325 aa
III
FN FN
FN FN
III III
III III
FN FN
FN FN
III III
III III
INFR1 489 aa
IL-22R1 574 aa
INFR2 332 aa
FN FN
FN
III III
III
FN FN
FN
III III
III
IL-13R1 427 aa
.
6
Embryonic stem cells
Sperm donor Egg donor
Egg fertilization
Culture fertilized cells for several cell divisions
Dissociate cell cluster for subculturing and
Ca
Ca Ca Ca
Ca
Ca
Ca CaM
Ca Ca
Ca Ca Ca
Ca
MLCK
Ca
Ca
Ca
.
Ca
CaM
Ca
P MLCK
P
myosin
myosin
P
myosin
1
RGS function and regulation
Ligand
S
GDP RGS
TNF
Ligand
S
GTP
RGS
P
RGS
S
GTP AC AC
binding
STAT
binding
Jak
Jak
3
3
STAT
binding
STAT
binding
Jak 3
STAT
binding
STAT
binding
SHP SHP-binding
3
Ig Ig - like
Adaptor proteins - selected (human)
1
SH3
SH2
217
SH3
BLNK
SLP-76
SHC-1
APS
845
SH3
Vav-1
SH3
Src-homology 3 domain
SH2
Src-homology 2 domain
SAM
C-1
SAM domain
Cysteine-rich region 1
Calponin
Calponin homology domain
Rho-GEF