间充质干细胞通过激活肝内胆管癌的自噬促进化疗抵抗机制研究

间充质干细胞通过激活肝内胆管癌的自噬促进化疗抵抗机制研究

摘要

本文旨在探讨间充质干细胞（MSCs）对肝内胆管癌治疗的影响及机制。通过实验结果发现，MSCs能够通过激活肝内胆管癌细胞的自噬作用，促进化疗抵抗能力。在体外实验中，使用间充质干细胞培养肝内胆管癌细胞，发现MSCs能够诱导肝内胆管癌细胞发生自噬，增加化疗药物的释放，提高肿瘤细胞的化疗敏感性。在实验中，使用MDC染色、EGFP-LC3标记、Western blot等方法，证实了MSCs通过激活肝内胆管癌细胞的自噬，促进化疗抵抗的作用。该研究为探索肝内胆管癌的治疗机制提供了新的思路和方法。

关键词：间充质干细胞；肝内胆管癌；自噬；化疗；抵抗机制Abstract

The aim of this paper is to explore the effect and mechanism of mesenchymal stem cells (MSCs) on the treatment of intrahepatic cholangiocarcinoma. Experimental results showed that MSCs can promote chemotherapy resistance by activating autophagy in intrahepatic cholangiocarcinoma cells. In vitro experiments, intrahepatic cholangiocarcinoma cells

were co-cultured with MSCs, and it was found that MSCs could induce autophagy in intrahepatic cholangiocarcinoma cells, increase the release of chemotherapy drugs, and improve the sensitivity of tumor cells to chemotherapy. In the experiment, MDC staining, EGFP-LC3 labeling, Western blot and other methods were used to verify that MSCs promote chemotherapy resistance by activating autophagy in intrahepatic cholangiocarcinoma cells. This study provides new ideas and methods for exploring the treatment mechanism of intrahepatic cholangiocarcinoma.

Keywords: Mesenchymal stem cells; Intrahepatic cholangiocarcinoma; Autophagy; Chemotherapy; Resistance mechanis

Chemotherapy resistance is a major obstacle in the treatment of intrahepatic cholangiocarcinoma, which is a highly aggressive and deadly form of liver cancer. Mesenchymal stem cells (MSCs) have been shown to play

a role in promoting chemotherapy resistance in various types of cancer, including intrahepatic cholangiocarcinoma, but the underlying mechanisms are not fully understood.

In this study, the researchers investigated the role

of autophagy in mediating the MSC-induced chemotherapy

resistance in intrahepatic cholangiocarcinoma. Autophagy is a cellular process that involves the degradation of damaged or unwanted cellular components to maintain cellular homeostasis. It has been shown to play a role in cancer development and progression, as well as in the response to chemotherapy.

The researchers found that MSCs promoted autophagy in intrahepatic cholangiocarcinoma cells, which resulted in increased chemotherapy resistance. This was confirmed by the MDC staining and EGFP-LC3 labeling, which are commonly used to detect and monitor autophagy. Moreover, the Western blot analysis showed that the expression levels of autophagy-related proteins, such as Beclin 1 and LC3, were upregulated in the intrahepatic cholangiocarcinoma cells co-cultured with MSCs.

To further confirm the role of autophagy in mediating the chemotherapy resistance, the researchers used autophagy inhibitors to suppress the autophagy

activity in the intrahepatic cholangiocarcinoma cells. They found that the chemotherapy sensitivity was significantly increased in the cells treated with autophagy inhibitors, indicating that autophagy plays a key role in promoting chemotherapy resistance.