N2神经细胞生长添加剂
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Reagent Series
Protocol for N2 Supplement
Applications
N2 Supplement is an optimised serum free supplement used in combination with B27 NeuroMix for:
•Differentiation of ES cells into neuron lineage (neuron and astrocytes)
•Differentiation of neural stem cells into astrocytes and neurons
•Optimal for serum free growth for neuroblastomas
•Use in combination with B27 NeuroMix
Shelf Life and Storage
N2 Supplement is stable for 1 year when stored at ≤-15°C.
Instructions for use
N2 Supplement is a 100 fold concentrate. Dilute N2 Supplement (100x) in the base medium 1:100. The final concentration of N2 Supplement corresponds to 1x. For preparation of 100 ml medium add 1 ml N2 Supplement (100x) into 99 ml of the appropriate base medium.
Cell culture vessels must be coated with poly-D-lysine (0.05 mg/ml). Fibronectin must be added at a final concentration of 5-10 µg/ml directly to the medium.
For serum free growth of neuroblastomas add N2 Supplement (100x) into Neuronal Base Medium (supplemented with 0.5 mM L-glutamine and 25 µM glutamate) to a final concentration of 1x.
For in vitro laboratory use or further manufacturing only. Not for human use.