Invitrogen中国测序通用引物序列
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pFlag-CMV-R:
GCA CTG GAG TGG CAA CTT
pIRES-F:
CTT ACT GAC ATC CAC TTT GC
pIRES.R:
CAC TGC ATT CTA GTT GTG GT
P1
CCA GGC TTT ACA CTT TAT GC
P2
GCG ATT AAG TTG GGT AAC GC
物名称
序列(5'-3')
M13R
CAG GAA ACA GCT ATG ACC
M13F
TGT AAA ACG ACG GCC AGT
M13F(-47)
CGC CAG GGT TTT CCC AGT CAC GAC
M13R(-48)
AGC GGA TAA CAA TTT CAC ACA GGA
M13(-96)
pDsRED-express-C1-F
TCC CAC AAC GAG GAC TAC AC
pCMV5R
ATT ATA GAG GAC ACC TAG TC
pCMV5F
TTC CAA AAT GTC GTA ATA AC
P5'
TGC GTA CTG CGG TGA TCA AC
P3'
CTG CAA GGC GAT TAA GTT GG
pIRES2-EGFP.P3’:
AAC GCA CAC CGG CCT TAT TC
3'AD:
AGA TGG TGC ACG ATG CAC AG
CMV -F
CGC AAA TGG GCG GTA GGC GTG
S1
CAA CGT GAA AAA ATT ATT ATT CGC
S6
GTA AAT GAA TTT TCT GTA GTA GG
pEGFP-C-5’:
CAT GGT CCT GCT GGA GTT CGT G
pEGFP-C-3' :
TAT GGC TGA TTA TGA TCA GT
PBV220F:
AAG AAG GGC AGC ATT CAA AG
PBV220R:
CTG CGT TCT GAT TTA ATC TG
U6
ATG GAC TAT CAT ATG CTT ACC GTA
5`AOX1
GAC TGG TTC CAA TTG ACA AGC
3`AOX1
GCA AAT GGC ATT CTG ACA TCC
α-Factor
TAC TAT TGC CAG CAT TGC TGC
GAL4 AD
TAC CAC TAC AAT GGA TG
pACT2-R
GTGCACGATGCACAGTTGAA
RVP3
CTA GCA AAA TAG GCT GTC CC
pQE30-R
GTT CTG AGG TCA TTA CTG G
pQE30-F
TGA GCG GAT AAC AAT TTC AC
pEF-F
TCA AGC CTC AGA CAG TGG TTC
pDSRED-N1-R
TGA AGC GCA TGA ACT CCT TG
BAC2
ACG CAC AGA ATC TAG CGC TT
BAC1
AAC CAT CTC GCA AAT AAA TA
3'BD:
TAA GAG TCA CTT TAA AAT TTG TAT AC
pTarget.F
CCA GGA TTT TCC CAG TCA C
pTarget.R
GGC TTT ACA CTT TAT GCT TC
pB42ADF:
CCA GCC TCT TGC TGA GTG GAG ATG
pB42ADR:
AAG CCG ACA ACC TTG ATT GGA G
pEGFP-N-5’:
TGG GAG GTC TAT ATA AGC AGA G
pEGFP-N-3’:
CGT CGC CGT CCA GCT CGA CCA G
T7(17bwk.baidu.comse)
ACA TCC ACT TTG CCT TTC TC
pYes2.R
TCG GTT AGA GCG GAT GTG
GAL4 BD
TCA TCG GAA GAG AGT AG
pAS2-1.R
AAG AGT TAC TCA AGA ACA AGA A
pFlag-CMV-F:
GGT AGG CGT GTA CGG TGG
GGT CGT CAG ACT GTC GAT GAA GCC
GGG CTG GCA AGC CAC GTT TGG TG
pGEX-4T-3'
CCG GGA GCT GCA TGT GTC AGA GG
GLp1
TGT ATC TTA TGG TAC TGT AAC TG
GLp2
CTT TAT GTT TTT GGC GTC TTC CA
RVp3
CTA GCA AAA TAG GCT GTC CC
pLEXA-F
CGT CAG CAG AGC TTC ACC AT
pLEXA-R
TAA AAC CTA AGA GTC ACT TT
pLNCX-F
AGC TCG TTT AGT GAA CCG TCA GAT CG
pLNCX-R
ACC TAC AGG TGG GGT CTT TCA TTC CC
pLXSN-F
CCC TCA TAG TTA GCG TAA CG
SP6
ATT TAG GTG ACA CTA TAG
T7
TAA TAC GAC TCA CTA TAG GG
T7 terminator
TGC TAG TTA TTG CTC AGC GG
T3
ATT AAC CCT CAC TAA AGG GA
pGEX-4T-5'
CTT GAA CCT CCT CGT TCG AC
pLXSN-R
GTT GCT GAC TAA TTG AGA TG
pSHUTTLE-CMV-F
GGT CTA TAT AAG CAG AGG TG
pSHUTTLE-CMV-R
GTG GTA TGG CTG ATT ATG ATC AG
MalE Primer
2.0rev primer:
AGG CGA TTA AGT TGG GTA
3.0rev:
GAG TTA GCT CAC TCA TTA GGC
S.tag
GAA CGC CAG CAC ATG GAC
5'TriplEx2
CTC CGA GAT CTG GAC GAG C
RVP4
GAC GAT AGT CAT GCC CCG CG
pTRC99C-F:
TTG CGC CGA CAT CAT AAC
pTRC99C-R:
CTGCGTTCTGATTTAATCTG
pCEP-F:
AGA GCT CGT TTA GTG AAC CG
EBV-R :
GTG GTT TGT CCA AAC TCA TC
pIRES2-EGFP.P5’:
GTA GGC GTG TAC GGT GGG AG
RVp4
GAC GAT AGT CAT GCC CCG CG
pcDNA3.1R
TAG AAG GCA CAG TCG AGG
PinPoint primer
CGT GAC GCG GTG CAG GGC G
pCMV-F
TCT AAA AGC TGC GGA ATT GT
pCMV-R
TCCAAACTCATCAATGTATC
GCA CTG GAG TGG CAA CTT
pIRES-F:
CTT ACT GAC ATC CAC TTT GC
pIRES.R:
CAC TGC ATT CTA GTT GTG GT
P1
CCA GGC TTT ACA CTT TAT GC
P2
GCG ATT AAG TTG GGT AAC GC
物名称
序列(5'-3')
M13R
CAG GAA ACA GCT ATG ACC
M13F
TGT AAA ACG ACG GCC AGT
M13F(-47)
CGC CAG GGT TTT CCC AGT CAC GAC
M13R(-48)
AGC GGA TAA CAA TTT CAC ACA GGA
M13(-96)
pDsRED-express-C1-F
TCC CAC AAC GAG GAC TAC AC
pCMV5R
ATT ATA GAG GAC ACC TAG TC
pCMV5F
TTC CAA AAT GTC GTA ATA AC
P5'
TGC GTA CTG CGG TGA TCA AC
P3'
CTG CAA GGC GAT TAA GTT GG
pIRES2-EGFP.P3’:
AAC GCA CAC CGG CCT TAT TC
3'AD:
AGA TGG TGC ACG ATG CAC AG
CMV -F
CGC AAA TGG GCG GTA GGC GTG
S1
CAA CGT GAA AAA ATT ATT ATT CGC
S6
GTA AAT GAA TTT TCT GTA GTA GG
pEGFP-C-5’:
CAT GGT CCT GCT GGA GTT CGT G
pEGFP-C-3' :
TAT GGC TGA TTA TGA TCA GT
PBV220F:
AAG AAG GGC AGC ATT CAA AG
PBV220R:
CTG CGT TCT GAT TTA ATC TG
U6
ATG GAC TAT CAT ATG CTT ACC GTA
5`AOX1
GAC TGG TTC CAA TTG ACA AGC
3`AOX1
GCA AAT GGC ATT CTG ACA TCC
α-Factor
TAC TAT TGC CAG CAT TGC TGC
GAL4 AD
TAC CAC TAC AAT GGA TG
pACT2-R
GTGCACGATGCACAGTTGAA
RVP3
CTA GCA AAA TAG GCT GTC CC
pQE30-R
GTT CTG AGG TCA TTA CTG G
pQE30-F
TGA GCG GAT AAC AAT TTC AC
pEF-F
TCA AGC CTC AGA CAG TGG TTC
pDSRED-N1-R
TGA AGC GCA TGA ACT CCT TG
BAC2
ACG CAC AGA ATC TAG CGC TT
BAC1
AAC CAT CTC GCA AAT AAA TA
3'BD:
TAA GAG TCA CTT TAA AAT TTG TAT AC
pTarget.F
CCA GGA TTT TCC CAG TCA C
pTarget.R
GGC TTT ACA CTT TAT GCT TC
pB42ADF:
CCA GCC TCT TGC TGA GTG GAG ATG
pB42ADR:
AAG CCG ACA ACC TTG ATT GGA G
pEGFP-N-5’:
TGG GAG GTC TAT ATA AGC AGA G
pEGFP-N-3’:
CGT CGC CGT CCA GCT CGA CCA G
T7(17bwk.baidu.comse)
ACA TCC ACT TTG CCT TTC TC
pYes2.R
TCG GTT AGA GCG GAT GTG
GAL4 BD
TCA TCG GAA GAG AGT AG
pAS2-1.R
AAG AGT TAC TCA AGA ACA AGA A
pFlag-CMV-F:
GGT AGG CGT GTA CGG TGG
GGT CGT CAG ACT GTC GAT GAA GCC
GGG CTG GCA AGC CAC GTT TGG TG
pGEX-4T-3'
CCG GGA GCT GCA TGT GTC AGA GG
GLp1
TGT ATC TTA TGG TAC TGT AAC TG
GLp2
CTT TAT GTT TTT GGC GTC TTC CA
RVp3
CTA GCA AAA TAG GCT GTC CC
pLEXA-F
CGT CAG CAG AGC TTC ACC AT
pLEXA-R
TAA AAC CTA AGA GTC ACT TT
pLNCX-F
AGC TCG TTT AGT GAA CCG TCA GAT CG
pLNCX-R
ACC TAC AGG TGG GGT CTT TCA TTC CC
pLXSN-F
CCC TCA TAG TTA GCG TAA CG
SP6
ATT TAG GTG ACA CTA TAG
T7
TAA TAC GAC TCA CTA TAG GG
T7 terminator
TGC TAG TTA TTG CTC AGC GG
T3
ATT AAC CCT CAC TAA AGG GA
pGEX-4T-5'
CTT GAA CCT CCT CGT TCG AC
pLXSN-R
GTT GCT GAC TAA TTG AGA TG
pSHUTTLE-CMV-F
GGT CTA TAT AAG CAG AGG TG
pSHUTTLE-CMV-R
GTG GTA TGG CTG ATT ATG ATC AG
MalE Primer
2.0rev primer:
AGG CGA TTA AGT TGG GTA
3.0rev:
GAG TTA GCT CAC TCA TTA GGC
S.tag
GAA CGC CAG CAC ATG GAC
5'TriplEx2
CTC CGA GAT CTG GAC GAG C
RVP4
GAC GAT AGT CAT GCC CCG CG
pTRC99C-F:
TTG CGC CGA CAT CAT AAC
pTRC99C-R:
CTGCGTTCTGATTTAATCTG
pCEP-F:
AGA GCT CGT TTA GTG AAC CG
EBV-R :
GTG GTT TGT CCA AAC TCA TC
pIRES2-EGFP.P5’:
GTA GGC GTG TAC GGT GGG AG
RVp4
GAC GAT AGT CAT GCC CCG CG
pcDNA3.1R
TAG AAG GCA CAG TCG AGG
PinPoint primer
CGT GAC GCG GTG CAG GGC G
pCMV-F
TCT AAA AGC TGC GGA ATT GT
pCMV-R
TCCAAACTCATCAATGTATC