经典猪瘟病毒母源抗体的衰减规
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F1场： 随日期，产房存栏仔猪 每日成活率分析： F1: the daily survival rate in farrowing house 随日期，保育存栏仔猪 每日成活率分析：daily survival rate in nursery
二、实验目的与方法 Objectives and Methods
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS negative farm turns to positive farm
F2场： 随日期，产房存栏仔猪 每日成活率分析： F2: daily survival rate s in farrowing 随日期，保育存栏仔猪 每日成活率分析： daily survival in nursery
F2场： 母猪随日期流产情况： F2 field: Sow abortion by date
母猪随月份均窝产活 仔情况average live born by month
二、实验目的与方法 Objectives and Methods
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS negative farm turns to positive farm
F1场2014年 9月13日出现母猪流产，随后产房保育仔猪成活率大幅下降，经PCR检测 并测序诊断为PRRSV野毒感染，繁殖群于11月20免疫 xx苗，至2015年 1月母猪繁殖恢 复至正常；(下附生产曲线图）F1: sow abortion on Sep. 13th, 2014, the survival rate in farrowing
4、母源抗体下降速度对断奶仔猪后期感染的影响：
The effect the decline rate of maternal antibodies on late infection in weaning pigs
文献显示，断奶后仔猪母源抗体消失 末期，保育期感染后的抗蓝耳病中和 抗体开始出现并上升。可为临床免疫 蓝耳弱毒苗提供依据。Literatures show that, in the late stage of maternal antibodies diminishing, anti-PRRS neutralizing antibodies began to appear and rise in infected nursery pigs, which provides support for clinical use attenuated PRRS vaccine. 图：(台湾) 6个不同 母猪场断奶后仔猪不 同周龄的蓝耳病抗体 阳性率及同期中和抗 体滴度。母源抗体下 降较慢的场，感染较 晚。HT LIU, 2008 Figure: (Taiwan) Percentages of weaning pigs with Ab positive and SN titers in pigs of different age group in six farms. Late infection was observed when maternal antibodies decline slowly in a farm.
二、实验目的与方法 Objectives and Methods
（一）目的 Objectives 1、找出母源PRRSV抗体在断奶后仔猪体内的衰减规律： To find out the trend of PRRS maternal antibody decay in weaning
pigs
2、确定仔猪断奶后感染PRRSV的时间：To determine the
（二）、材料与方法 Materials and Methods
1、猪群背景： Herd Background: F1场为1800繁殖母猪带保育-肥育一体的猪场。 F1 farm is a 1800 sow multiplication farm with nursery-finishing F2场为3600头母猪场，其断奶后的保育肥育猪分置于直线约10km外集中饲养。 三个场原均为 PRRSV抗原抗体双阴性群。 F2 is a 3600 sow multiplication farm, weaned pigs are put nursery/finisher 10 km away. All three farms are PRRSV double negative.
二、实验目的与方法 Objectives and Methods
abortion
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS
negative farm turns positive farm
Live born
F1场： 母猪随日期流产情况： F1 : Sow abortion by date
一、母源抗体对断奶后仔猪的意义
The significance of maternal antibody on weaning pigs 1、母源抗体在断奶仔猪循环系统中作用：Effect of
maternal antibody on the circulatory system of weaning pigs.
PRRS infection after weaning
3、制订仔猪PRRSV免疫方案。 To develop PRRSV
immunization protocol for piglets
4、找出抗生素干预时机。to Identify the timing of antibiotic
intervention
5、确定仔猪转群时间 。To determine the time for transferring piglets 6、是否修订繁殖猪群免疫方案 whether need to revise vaccination protocol for multiplication herd.
二、实验目的与方法 Objectives and Methods
母猪随月份均窝产活 仔情况 average live born by month
二、实验目的与方法 Objectives and Methods
abortion
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS negative farm turns to positive farm
Live born
2、仔猪断奶后母源蓝耳病抗体的消失规律的意义：
The significance of PRRS maternal antibody decay after weaning
3、提前免疫弱毒活疫苗对生产的帮助：Effect of early
vaccination using attenuated live vaccine on production
dropped significantly, diagnosed with PRRSV wild virus infection by PCR and sequencing, breeding herd was inoculated with xx vaccine on Nov. 20th., sows reproduction returned to normal by January 2015; (Production curve is provided)
实验目的与方法
Objectives and Methods
实验结果与生产性能分析 Results and analysis of
production performance
启示Implications
第一部分 Part 1
断奶后仔猪繁殖与呼吸综合征病毒(PRRSV) 母源抗体的衰减试验
Experiment on Maternal Antibody Decay of Porcine Reproductive and Respiratory Syndrome Virus in Weaning Nursery Pigs
F2场 2014年 9 月26日出现母猪流产，随后产房保育仔猪成活率下降， 经PCR检测并测 序同为PRRSV野毒感染，随后繁殖群于10月1日免疫 xx苗， 至2014年 12月母猪繁殖 恢复至正常；(下附生产曲线图）F2:sow abortion on Sep. 26th, 2014, diagnosed with PRRSV wild virus infection PCR and sequencing, the breeding herd was inoculated with xx vaccine on Oct. 1st., sows reproduction returned to normal by December 2014; (Production curve is provided)
猪繁殖呼吸综合征与经典猪瘟母源抗体衰减检测 及其对断奶仔猪免疫的应用 Trend of maternal antibody decay in nursery pigs for porcine reproductive and respiratory syndrome and classic swine fever viruses using serial paired field trial
李延明 Yanming Li 北京二商大红门科技牧业有限公司 2015年10月 南京 Beijing Two Business Da Hong Men Technology Husbandry Ltd., Co. China
主要内容 Content
3 一 二 3 三 四
母源抗体对断奶后仔猪的意义 The importance of maternal antibody to weaning pigs
二、实验目的与方法 Objectives and Methods
2、采血检测与分析方法：Blood test and analysis
（1）F1和F2场，选取同一单元分娩舍的断奶后仔猪16头和 30 头，记录来源母猪、出生日期， 平均约23日龄断奶。分别于3、4、5、6、7、8、9、12、13周龄及3、4、5、6、7、8、9、 13、14周龄连续采血，集中3批送检PRRSV抗体。(见下采血计划表）Select 16 and 30 weaned pigs from the same farrowing unit, record sows history, birth date, average weaning days-23 d, Collected blood samples respectively at 3, 4,5,6,7,8,9,12,13-week and 3, 4,5,6,7,8,9,13,14-week, sent out in 3 batches to test PRRSV antibody. （2）以上仔猪在9周龄以前不注射任何疫苗。 All piglets selected above were not vaccinated before 9-week old （3）以上仔猪如在试验过程中出现死亡、操作失误或溶血等现象，将退出试验。 In case of pig death, operational errors or hemolysis during experimental period, pigs will not be counted. （4）抗体检测用海博莱 PRRS 试剂盒，EIasa酶标酶联仪器 Antibody test kits: HIPRA PRRS & ELISA testing kit, （5）分析方法统一为 Analytic methods are a-不同日龄组仔猪的母源抗体阳性率+变化趋势；maternal antibody positive rate in pigs of different age group + trend b-不同日龄组仔猪的母源抗体水平+变动范围(四分位范围及中位数位置)；level of maternal antibody in pigs of different age groups + range (Interquartile range and median) c-仔猪断奶当时，按照抗体水平由高至低排列以后，后续周龄的抗体衰减趋势的差异 When weaning, ranking base on the level of maternal antibody from high to low, the difference in antibody decay trend in the following weeks
二、实验目的与方法 Objectives and Methods
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS negative farm turns to positive farm
F2场： 随日期，产房存栏仔猪 每日成活率分析： F2: daily survival rate s in farrowing 随日期，保育存栏仔猪 每日成活率分析： daily survival in nursery
F2场： 母猪随日期流产情况： F2 field: Sow abortion by date
母猪随月份均窝产活 仔情况average live born by month
二、实验目的与方法 Objectives and Methods
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS negative farm turns to positive farm
F1场2014年 9月13日出现母猪流产，随后产房保育仔猪成活率大幅下降，经PCR检测 并测序诊断为PRRSV野毒感染，繁殖群于11月20免疫 xx苗，至2015年 1月母猪繁殖恢 复至正常；(下附生产曲线图）F1: sow abortion on Sep. 13th, 2014, the survival rate in farrowing
4、母源抗体下降速度对断奶仔猪后期感染的影响：
The effect the decline rate of maternal antibodies on late infection in weaning pigs
文献显示，断奶后仔猪母源抗体消失 末期，保育期感染后的抗蓝耳病中和 抗体开始出现并上升。可为临床免疫 蓝耳弱毒苗提供依据。Literatures show that, in the late stage of maternal antibodies diminishing, anti-PRRS neutralizing antibodies began to appear and rise in infected nursery pigs, which provides support for clinical use attenuated PRRS vaccine. 图：(台湾) 6个不同 母猪场断奶后仔猪不 同周龄的蓝耳病抗体 阳性率及同期中和抗 体滴度。母源抗体下 降较慢的场，感染较 晚。HT LIU, 2008 Figure: (Taiwan) Percentages of weaning pigs with Ab positive and SN titers in pigs of different age group in six farms. Late infection was observed when maternal antibodies decline slowly in a farm.
二、实验目的与方法 Objectives and Methods
（一）目的 Objectives 1、找出母源PRRSV抗体在断奶后仔猪体内的衰减规律： To find out the trend of PRRS maternal antibody decay in weaning
pigs
2、确定仔猪断奶后感染PRRSV的时间：To determine the
（二）、材料与方法 Materials and Methods
1、猪群背景： Herd Background: F1场为1800繁殖母猪带保育-肥育一体的猪场。 F1 farm is a 1800 sow multiplication farm with nursery-finishing F2场为3600头母猪场，其断奶后的保育肥育猪分置于直线约10km外集中饲养。 三个场原均为 PRRSV抗原抗体双阴性群。 F2 is a 3600 sow multiplication farm, weaned pigs are put nursery/finisher 10 km away. All three farms are PRRSV double negative.
二、实验目的与方法 Objectives and Methods
abortion
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS
negative farm turns positive farm
Live born
F1场： 母猪随日期流产情况： F1 : Sow abortion by date
一、母源抗体对断奶后仔猪的意义
The significance of maternal antibody on weaning pigs 1、母源抗体在断奶仔猪循环系统中作用：Effect of
maternal antibody on the circulatory system of weaning pigs.
PRRS infection after weaning
3、制订仔猪PRRSV免疫方案。 To develop PRRSV
immunization protocol for piglets
4、找出抗生素干预时机。to Identify the timing of antibiotic
intervention
5、确定仔猪转群时间 。To determine the time for transferring piglets 6、是否修订繁殖猪群免疫方案 whether need to revise vaccination protocol for multiplication herd.
二、实验目的与方法 Objectives and Methods
母猪随月份均窝产活 仔情况 average live born by month
二、实验目的与方法 Objectives and Methods
abortion
猪繁殖与呼吸综合征 由阴性场向阳性场转 化过程中：PRRS negative farm turns to positive farm
Live born
2、仔猪断奶后母源蓝耳病抗体的消失规律的意义：
The significance of PRRS maternal antibody decay after weaning
3、提前免疫弱毒活疫苗对生产的帮助：Effect of early
vaccination using attenuated live vaccine on production
dropped significantly, diagnosed with PRRSV wild virus infection by PCR and sequencing, breeding herd was inoculated with xx vaccine on Nov. 20th., sows reproduction returned to normal by January 2015; (Production curve is provided)
实验目的与方法
Objectives and Methods
实验结果与生产性能分析 Results and analysis of
production performance
启示Implications
第一部分 Part 1
断奶后仔猪繁殖与呼吸综合征病毒(PRRSV) 母源抗体的衰减试验
Experiment on Maternal Antibody Decay of Porcine Reproductive and Respiratory Syndrome Virus in Weaning Nursery Pigs
F2场 2014年 9 月26日出现母猪流产，随后产房保育仔猪成活率下降， 经PCR检测并测 序同为PRRSV野毒感染，随后繁殖群于10月1日免疫 xx苗， 至2014年 12月母猪繁殖 恢复至正常；(下附生产曲线图）F2:sow abortion on Sep. 26th, 2014, diagnosed with PRRSV wild virus infection PCR and sequencing, the breeding herd was inoculated with xx vaccine on Oct. 1st., sows reproduction returned to normal by December 2014; (Production curve is provided)
猪繁殖呼吸综合征与经典猪瘟母源抗体衰减检测 及其对断奶仔猪免疫的应用 Trend of maternal antibody decay in nursery pigs for porcine reproductive and respiratory syndrome and classic swine fever viruses using serial paired field trial
李延明 Yanming Li 北京二商大红门科技牧业有限公司 2015年10月 南京 Beijing Two Business Da Hong Men Technology Husbandry Ltd., Co. China
主要内容 Content
3 一 二 3 三 四
母源抗体对断奶后仔猪的意义 The importance of maternal antibody to weaning pigs
二、实验目的与方法 Objectives and Methods
2、采血检测与分析方法：Blood test and analysis
（1）F1和F2场，选取同一单元分娩舍的断奶后仔猪16头和 30 头，记录来源母猪、出生日期， 平均约23日龄断奶。分别于3、4、5、6、7、8、9、12、13周龄及3、4、5、6、7、8、9、 13、14周龄连续采血，集中3批送检PRRSV抗体。(见下采血计划表）Select 16 and 30 weaned pigs from the same farrowing unit, record sows history, birth date, average weaning days-23 d, Collected blood samples respectively at 3, 4,5,6,7,8,9,12,13-week and 3, 4,5,6,7,8,9,13,14-week, sent out in 3 batches to test PRRSV antibody. （2）以上仔猪在9周龄以前不注射任何疫苗。 All piglets selected above were not vaccinated before 9-week old （3）以上仔猪如在试验过程中出现死亡、操作失误或溶血等现象，将退出试验。 In case of pig death, operational errors or hemolysis during experimental period, pigs will not be counted. （4）抗体检测用海博莱 PRRS 试剂盒，EIasa酶标酶联仪器 Antibody test kits: HIPRA PRRS & ELISA testing kit, （5）分析方法统一为 Analytic methods are a-不同日龄组仔猪的母源抗体阳性率+变化趋势；maternal antibody positive rate in pigs of different age group + trend b-不同日龄组仔猪的母源抗体水平+变动范围(四分位范围及中位数位置)；level of maternal antibody in pigs of different age groups + range (Interquartile range and median) c-仔猪断奶当时，按照抗体水平由高至低排列以后，后续周龄的抗体衰减趋势的差异 When weaning, ranking base on the level of maternal antibody from high to low, the difference in antibody decay trend in the following weeks