胃癌细胞原代培养和Ming氏浸润型胃癌细胞系的建立
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1．胃癌标本 ······················································································6 2．实验动物 ······················································································6 3．主要仪器设备 ················································································6 4．主要试剂 ······················································································7 5．常用溶液配制 ················································································8 二、方法 ································································································8 (一) 细胞原代培养与传代培养······························································8 1．培养皿及器械的处理与准备·····························································8 2．胃癌细胞原代培养 ·········································································9 3．纯化与传代 ·················································································10 （二）Ming 氏浸润型胃癌细胞系的建立和特性 ······································10 1．细胞系的建立··············································································10 2．细胞系的特性··············································································10
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万方数据ቤተ መጻሕፍቲ ባይዱ
结 果······························································································15 一、胃癌细胞原代培养···········································································15 二、Ming 氏浸润型胃癌细胞系的特性 ······················································19 1．形态学 ····························································································20 2．细胞免疫组化···················································································22 3．过碘酸雪夫染色 ···············································································23 4．倍增时间 ·························································································23 5．核型分析 ·························································································24 6．琼脂糖实验······················································································25 7．裸鼠体内成瘤实验 ············································································25 讨 论······························································································27 一、胃癌原代培养条件···········································································27 二、Ming 氏浸润型胃癌细胞系的特性 ······················································29 三、染色体异常与胃癌发生的关系···························································31 结 论······························································································33 参考文献······························································································34 致 谢······························································································39 综 述······························································································40
万方数据
目录
缩写词表 ································································································1 中文摘要 ································································································2 英文摘要 ································································································3 引 言 ································································································5 材料和方法·····························································································6 一、材料 ································································································6
2.1 形态学 ···················································································11 2.2 细胞免疫组化··········································································11 2.3 过碘酸雪夫染色·······································································12 2.4 细胞倍增实验··········································································12 2.5 核型分析 ················································································12 2.6 琼脂糖克隆实验·······································································12 2.7 裸鼠体内成瘤实验 ···································································13 （三）数据分析 ·················································································13
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万方数据ቤተ መጻሕፍቲ ባይዱ
结 果······························································································15 一、胃癌细胞原代培养···········································································15 二、Ming 氏浸润型胃癌细胞系的特性 ······················································19 1．形态学 ····························································································20 2．细胞免疫组化···················································································22 3．过碘酸雪夫染色 ···············································································23 4．倍增时间 ·························································································23 5．核型分析 ·························································································24 6．琼脂糖实验······················································································25 7．裸鼠体内成瘤实验 ············································································25 讨 论······························································································27 一、胃癌原代培养条件···········································································27 二、Ming 氏浸润型胃癌细胞系的特性 ······················································29 三、染色体异常与胃癌发生的关系···························································31 结 论······························································································33 参考文献······························································································34 致 谢······························································································39 综 述······························································································40
万方数据
目录
缩写词表 ································································································1 中文摘要 ································································································2 英文摘要 ································································································3 引 言 ································································································5 材料和方法·····························································································6 一、材料 ································································································6
2.1 形态学 ···················································································11 2.2 细胞免疫组化··········································································11 2.3 过碘酸雪夫染色·······································································12 2.4 细胞倍增实验··········································································12 2.5 核型分析 ················································································12 2.6 琼脂糖克隆实验·······································································12 2.7 裸鼠体内成瘤实验 ···································································13 （三）数据分析 ·················································································13