prime star 说明书
takara primestar GXL 酶 操作手册
Cat. #R050AProduct ManualPrimeSTAR® GXL DNA PolymeraseFor Research Usev1108DaTable of ContentsI. Description (3)II. Components (3)III. Storage (3)IV. Protocols (4)V. Optimization of Parameters (6)VI. Electrophoresis, Cloning, and Sequencing of Amplified Products (7)VII. Troubleshooting (7)VIII. Features (8)IX. Related Products (11)I. DescriptionPrimeSTAR GXL DNA Polymerase is a revolutionary PCR enzyme that augments the high-fidelity PrimeSTAR HS DNA Polymerase by modification with a novel elongation factor to dramatically increase PCR performance.The superior performance of PrimeSTAR GXL DNA Polymerase is unsurpassed by other commercially available high-fidelity PCR enzymes. PrimeSTAR GXL DNA Polymerase allows amplification of products ≧30 kb in length while maintaining exceptionally high fidelity. Suitable for GC-rich templates that are otherwise difficult to amplify, this enzyme enables successful amplification from challenging templates without need for extensive optimization of buffers or reaction conditions. In addition, PrimeSTAR GXL DNA Polymerase is compatible with a wide template quantity range, and is capable of robust amplification even in the presence of large excesses of non-target DNA. Excess non-target nucleic acid typically inhibits performance of conventional high-fidelity PCR enzymes. With PrimeSTAR GXL DNA Polymerase, detection of cDNA corresponding to rare transcripts is readily achieved.Furthermore, an antibody-mediated hot start formulation prevents false initiation events during reaction assembly due to mispriming and primer digestion.The PCR extension time recommended in the Standard Protocol is 1 min./kb. However, by doubling the quantity of enzyme used, it is also possible to perform rapid PCR on a wide range of targets using an extension time of 10 sec./kb.II. Components (200 reactions)＊1PrimeSTAR GXL DNA Polymerase (1.25 U/µl) 200 µl5X PrimeSTAR GXL Buffer (Mg2+ plus)＊2 1 ml x 2dNTP Mixture（2.5 mM each）800 µl＊1 : Assuming a 50 µl reaction volume＊2 : Mg2+ concentration: 5 mM (5X).III. Storage–20℃IV. ProtocolsTwo protocols are described: a Standard Protocol, in which an extension time of 1 min./kb isused, and a Rapid PCR Protocol, in which extension can be conducted at 10 sec./kb by using twice the quantity of enzyme.PCR reaction mixtures can be prepared at room temperature. However, keep each of thereaction components on ice while preparing the reaction mixture.A. Standard protocol・Composition of PCR Reaction MixtureFinal conc.5X PrimeSTAR GXL Buffer10 µl1XdNTP Mixture (2.5 mM each) 4 µl200 μM eachprimer 110 - 15 pmol0.2 - 0.3 µM＊primer 210 - 15 pmol0.2 - 0.3 µM＊Template Refer to V.3. Optimization of ParametersPrimeSTAR GXL DNA Polymerase 1 µl 1.25 U/50 µlSterilized distilled water to final reaction volume of 50 µl＊: When amplifying products ≧ 10 kb in length, use primers at a finalconcentration of 0.2 µM each.・PCR Conditions[For ≦ 10 kb products]98℃10 sec.55 or 60℃＊115 sec. 30 cycles [3-step PCR]68℃＊2 1 min./kb- or -98℃10 sec.30 cycles [2-step PCR]68℃＊2 1 min./kb＊1: When the T m value (calculated by the following formula＊) is greaterthan 55 ℃, set the annealing temperature to 60℃. When the T m valueis 55℃ or less, set the annealing temperature to 55℃.＊ : T m value calculation method:T m (℃) = 2(NA + NT) + 4(NC + NG) - 5where N represents the number of primer nucleotides having thespecified identity (A, T, C, or G)＊2: For both 2-step and 3-step PCR, set the extension temperature to 68℃.[For 10 to 30 kb products]98℃10 sec.30 cycles68℃10 min.[For ≧30 kb products]98℃10 sec.30 cycles68℃15 min.◆Selecting PCR conditions• For amplification of products ≦10 kb in length, try 3-step PCR first.• When using GC-rich templates or amplifying products ≧10 kb in length,2-step PCR is recommended.• If amplified products are not obtained or if smeared or non-specific band(s)are observed during electrophoresis analysis, refer to VII. Troubleshooting.B. Rapid PCR Protocol・ Composition of PCR Reaction MixtureFinal conc.5X PrimeSTAR GXL Buffer10 µl 1X dNTP Mixture（2.5 mM each） 4 µl 200 µM eachprimer 110 - 15 pmol 0.2 - 0.3 µM *primer 210 - 15 pmol 0.2 - 0.3 µM *TemplateRefer to V.3. Optimization of ParametersPrimeSTAR GXL DNA Polymerase 2 µl 2.5 U/50 µlSterilized distilled waterto final reaction volume of 50 µl＊: When amplifying products ≧ 10 kb in length, use primers at a finalconcentration of 0.2 µM each.・ PCR Conditions[For ≦ 10 kb products]98℃10 sec.55 or 60℃＊115 sec. 30 cycles [3-step PCR]68℃＊210 sec./kb＊1: When the T m value (calculated by the following formula ＊) is morethan 55 ℃, set the annealing temperature to 60℃. When the T m value is 55℃ or less, set the annealing temperature to 55℃.＊ : T m value calculation method:T m (℃) = 2(NA + NT) + 4(NC + NG) - 5where N represents the number of primer nucleotides having the specified identity (A, T, C, or G)＊2: For 3-step PCR, set the extension temperature to 68℃.[For 10 to 20 kb products]98℃68℃10 sec.20 sec./kb 30 cycles [2-step PCR]- or - 98℃60℃68℃10 sec.15 sec.10 sec./kb30 cycles [3-step PCR]◆Selecting PCR conditions• For amplification of products ≦10 kb in length, perform 3-step PCR. 2-step PCR is not recommended for products of this size.• For amplification of products ≧10 kb in length, 3-step PCR is recommended when a shorter reaction time is desired, and 2-step PCR is recommended when enhanced specificity is desired.• For GC-rich templates, use the Standard Protocol.• If amplified products are not obtained or if smeared or non-specific band(s) are observed in electrophoresis analysis, refer to VII. Troubleshooting.V. Optimization of ParametersIn order to obtain the best PCR results, it is important to optimize the PrimeSTAR GXLDNA Polymerase reaction parameters to fully utilize the enzyme's properties and ad-vantages.(1) Primer designSelect primer sequences using primer design software such as OLIGO™ Primer AnalysisSoftware (Molecular Biology Insights, Inc.).[For ≦10 kb products]For general amplification, 20 to 25-mer primers are suitable. Selection of primers witha T m value of ≧55℃ (as calculated using the formula in IV. PCR Conditions) or greaterthan 25-mer in length may provide optimal results. See IV. Protocols.Do not use inosine-containing primers with PrimeSTAR GXL DNA Polymerase.[For >10 kb products]Design primers that are 25- to 35-mers and that have a T m value of ≧65℃.Avoid high GC content at the 3' end of each primer.[For GC-rich amplification products]Design primers to have T m values > 60℃.Note:Do not use inosine-containing primers with PrimeSTAR GXL DNA Polymerase.(2) dNTP and Mg2+dNTPs are capable of chelation, and therefore the concentration of un-chelated (free)Mg2+ in a reaction mix is inversely related to dNTP concentration. The PrimeSTAR GXLBuffer is formulated to result in a final (1X) concentration of 1 mM Mg2+ when final (1X)concentration of dNTPs is 200 μM each. Avoid changing the dNTP concentration asmuch as possible.Do not use dUTP with PrimeSTAR GXL DNA Polymerase. dUTP will greatly affectenzyme activity.(3) TemplateRecommended quantities of template DNA (assuming a 50 μl reaction):(for general conditions)(for long PCR products) Human genomic DNA 5 ng - 500 ng（100 ng - 500 ng）E. coli genomic DNA100 pg - 200 ng（10 ng - 200 ng）Plasmid DNA10 pg - 10 ng（1 ng - 10 ng）cDNA25 ng - 750 ng（250 ng - 750 ng）Do not use templates containing uracil, such as bisulfite-treated DNA.VI. Electrophoresis, Cloning, and Sequencing of Amplified Products(1) ElectrophoresisTAE Buffer is recommended for agarose gel electrophoresis of amplified products thatare obtained using PrimeSTAR GXL DNA Polymerase.Note : Use of TBE Buffer may result in DNA band patterns that are enlarged at the bot-tom of the gel .(2) Termini of amplified productsMost PCR products amplified with PrimeSTAR GXL DNA Polymerase have blunt-endtermini. Accordingly, they can be cloned directly into blunt-end vectors. If necessary,phosphorylate amplified products before cloning. Use Mighty Cloning Reagent Set(Blunt End) (Cat.# 6027)for cloning into a blunt-end vector.(3) Restriction enzyme digestionPrior to performing restriction enzyme digestion of amplified PCR products, re-move all traces of PrimeSTAR GXL DNA Polymerase from the reaction mixture byphenol/chloroform extraction or by using NucleoSpin® Extract II (Clontech Cat.#740609.10/.50/.250). Particularly for 3'-protruding restriction enzymes such as Pst I,the 3'-protruding termini produced by these enzymes may be deleted by 3' → 5' exo-nuclease activity of PrimeSTAR GXL DNA Polymerase, if residual polymerase remainspresent in the restriction digest reaction.(4) Direct sequencingPerform phenol/chloroform extraction of PCR products prior to direct sequencing toensure inactivation of 3' → 5' exonuclease activity. Alternatively, NucleoSpin® Extract II(Clontech Cat. #740609.10/.50/.250) may be used to purify DNA prior to sequencing.VII. TroubleshootingEvent Possible Causes ActionNo amplification orpoor amplification efficiency Primer T m Refer to V-(1) Optimization of Parameters -Primer DesignAnnealing temperature Lower by 2℃ per trialPrimer concentration Use in the range of 0.3 to 0.5 μM (final conc.) PCR conditions Try Rapid PCR ProtocolNumber of cycles Set to 35 to 40 cyclesPurity and quantity oftemplate DNAUse an appropriate amount of template DNA.Purify the template DNA.Electrophoresis analysis shows smeared band(s) or extra band(s)Primer T m Refer to V-(1) Optimization of Parameters -Primer DesignAnnealing temperatureRaise by 2℃ per trial up to to 63℃Try 2-step PCRFor a primer T m value of 50℃ or less,set in the range of 50 ℃ to 55℃Extension time When amplification product is ≦1 kb, setto 10 sec./kbPrimer concentration Use at a final concentration of 0.2 µM each Number of cycles Set to 25 to 30 cyclesTemplate DNA purity Purify the template DNAVIII. FeaturesA. AccuracyMutation frequency of PrimeSTAR GXL DNA Polymerase was examined by analysis of sequencing data.[ Method ]Ten arbitrarily selected GC-rich regions were amplified with PrimeSTAR GXL DNA Polymerase or other DNA polymerases using Thermus thermophilus HB8 genomic DNA as template.Each PCR product (approx. 500 bp each) was cloned into a suitable plasmid. Multi-ple clones were selected per respective amplification product and were subjected to sequence analysis.[ Result ]Sequence analysis of DNA fragments amplified using PrimeSTAR GXL DNA Poly-merase demonstrated 30 mismatched bases per 486,923 total bases. This is higher fidelity than Pfu DNA polymerase.B. Length of amplification productsPrimeSTAR GXL DNA Polymerase enables amplification of long DNA fragments that cannot be obtained using other commercially available high fidelity PCR enzymes. As shown below, amplification was confirmed for fragments up to 30 kb in length using human genomic DNA as template, fragments up to 40 kb in length using λDNA as template, and fragments up to 13.5 kb in length using cDNA as template.（％）PrimeSTAR ® Max PeimeSTAR ® HS PrimeSTAR ® GXL Pfu DNA Polymeraser Taq DNA Polymerase1. 0.5 kb2. 1 kb3. 2 kb4. 4 kb5. 8 kb6. 15 kb7. 20 kb8. 30 kb9. 40 kbM. λ-Hind III digestλDNA(Template: 1 ng/50 µl reaction)M 123456789M1. p53 0.5 kb2. DCLRE 1A 1 kb3. DCLRE 1A 2 kb4. DCLRE 1A 4 kb5. β-globin 8.5 kb6. β-globin 15 kb7. β-globin 20 kb8. β-globin 24 kb9. β-globin 27 kb 10. β-globin 30 kb M. λ-Hind III digestHuman genomic DNA(Template: 100 ng/50 µl reaction)M 12345678910M 1. Dystrophin 1 kb 2. Dystrophin 2 kb 3. CCND2 2.8 kb 4. TFR 4 kb 5. Dystrophin 6 kb 6. Dystrophin 8 kb 7. Dystrophin 12 kb 8. Dystrophin 13.5 kb M. λ-Hind III digest(equivalent of 250 ng total RNA/ 50 µl reaction)M 12345678McDNAC. Amplification of GC-rich targetsPrimeSTAR GXL DNA Polymerase allows highly specific amplification of GC-rich templates that are otherwise challenging. Excellent results are achieved without requiring special buffers or reaction conditions. The performance of PrimeSTAR GXL DNA Polymerase in comparison to other commercially available high-fidelity DNA polymerases and polymerases optimized for GC-rich templates is shown. Reactionswere performed according to the protocols specified by each manufacturer.M 1234M 1234M M 1234M 1234M 1234MM 1234M PrimeSTARGXLCompany A's enzymeCompany B's enzymeCompany B's enzyme 2Company C's enzymeCompany D's enzymeTemplate: Human genomic DNA (100 ng / 50 µl reaction)1. APOE gene 746 bp (GC content 74%)2. TGFβ1 gene 2,005 bp (GC content 69%)Template: T. thermophilus HB8 genomic DNA (10 ng / 50 µl reaction)3. 2029 bp (GC content 74%)4. 4988 bp (GC content 74%)M : pHY MarkerCompany B's enzyme 2:Optimized for GC-rich templates Company D's enzyme:includes buffers optimized for GC-rich templatesD. Sensitivity and wide template quantity rangeConventional high-fidelity PCR enzymes are relatively easily affected by excess nucleic acid in a reaction solution, and frequently do not readily amplify cDNA templates. In contrast, PrimeSTAR GXL DNA Polymerase shows excellent activity over a wide range of template quantities, and therefore is well-suited for efficient amplification of cDNA templates.(1) Using cDNA templates obtained by reverse transcription of various quantities oftotal RNA prepared from HL 60 cells, transferrin receptor (TFR) gene (4 kb) was amplified using each enzyme in the PrimeSTAR series. Sensitivity and template quantity range were compared.cDNA template quantity (equivalent to total RNA amounts indicated / 50 µl reaction) is as follows: 1. 25 pg 7. 750 ng 2. 250 pg 8. 1μg 3. 2.5 ng 9. 1.5μg 4. 25 ng 10. 2μg 5. 250 ng M. λ-Hin d III digest 6． 500 ngPrimeSTAR GXL DNA Polymerase demonstrated good amplification over a widerange of template cDNA quantity, as well as excellent sensitivity.MM 12345678910M M 12345678910M 12345678910PrimeSTAR GXLPrimeSTAR HSPrimeSTAR Max(2) Using various quantities of human genomic DNA as template, the amplification efficiency of PrimeSTAR GXL DNA Polymerase was compared to the efficiencies of other commercially available high fidelity PCR enzymes and Taq DNA Poly-merase. Reactions were performed according to the protocols specified by each manufacturer.Template quantity (/50 μl reaction)1. No template 2． 100 pg 3． 1 ng 4． 10 ng 5． 100 ng 6． 200 ng 7． 500 ng M．λ-Hind III digest Company A's enzyme PrimeSTAR GXL M 1234567M 1234567M Company C's enzymeCompany B's enzyme M 1234567M 1234567MCompany E's enzyme TaqM 1234567M 1234567M Template: Human genomic DNA Target: DCLRE 1A gene (2 kb)PrimeSTAR GXL DNA Polymerase demonstrated superior sensitivity and amplifi-cation efficiency in comparison to other commercially available high-fidelity PCR enzymes and Taq . High activity was observed for PrimeSTAR GXL DNA Poly-merase even in the presence of excess template DNA that suppressed the activity of high-fidelity PCR enzymes from other companies.IX. Related ProductsPrimeSTAR® HS DNA Polymerase (Cat. #R010A/B)PrimeSTAR® HS (Premix) (Cat. #R040A)PrimeSTAR® Max DNA Polymerase (Cat. #R045A)TaKaRa PCR Thermal Cycler Dice™ Gradient/Standard (Cat. #TP600/TP650)＊Mighty Cloning Reagent Set (Blunt end) (Cat. #6027)NucleoSpin® Extract II (Clontech Cat. #740609.10/.50/.250)＊ : not available in the U.S.NOTICE TO PURCHASER : LIMITED LICENSE[P1] PCR NoticeUse of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit underthe foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activitiesfor a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Fostcr City, California 94404, USA.[L15] Hot Start PCRLicensed under U.S. Patent No. 5.338,671 and 5,587,287, and corresponding patents in other countries.[M54] PrimeSTAR HS DNA PolymeraseThis product is covered by the claims of U.S. Patent No. 7,704,713 and its foreign counterparts.NOTE :This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, or household item, etc.Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC.Your use of this product is also subject to compliance with any applicable licensing require-ments described on the product web page at . It is your respon-sibility to review, understand and adhere to any restrictions imposed by such statements. If you require licenses for other use, please contact us by phone at +81 77 543 7247.。
prime操作手册 091203
Prima膝关节被动运动仪中文操作手册(2009版)目录Prima膝关节被动运动仪1. 适用症 (3)2. 临床优势 (3)3. 设备描述 (3)4. 电源连接：安全第一 (4)5. 安全 (4)6. 启动仪器 (5)7. 使用Kinetec患者衬垫套装 (5)8. 患者安放 (6)9. 脚板架位置 (6)10. 保修 (6)11. 清洁 (6)12. 销毁和回收 (7)13. 故障处理 (7)14. 技术参数 (7)15．符号使用 (8)16. 可选配件 (8)17. 保修 (10)Prima膝关节被动运动仪中文操作说明书1. 适用症●膝关节置换术●骨折（膝盖骨，胫骨，股骨，…）●关节松懈术●髋关节术，包括髋关节置换，髋关节固定，髋关节截骨术，…●韧带修复●关节镜外科（半月板切除术，髌骨切除术，…）●灼伤，化脓性关节炎，…2. 临床优势缩短外伤，炎症及关节活动度修复周期预防关节僵硬加速术后关节活动度的恢复保持关节连接面减少疼痛和水肿推动关节软骨康复缩短住院治疗时间减少止疼药物的摄入及时提供术后连续被动运动禁忌症骨癌，扭曲关节面，麻痹性偏瘫，粉碎性骨折，未受控制性传染。
该设备不适用于患者身高高于2米（6尺7寸），或者低于1.47米（4尺7寸）。
3. 设备描述KINETEC Prima Advance是款膝盖被动运动型设备，伸缩活动范围在-5°到115°之间。
A-下缘支柱B-大腿支柱C-脚板架D-控制面板E-手柄4. 电源连接：安全第一KINETEC Prima Advance是Ⅰ类B型设备。
把KINETEC Prima Advance的电源线（1）接到插座上（电压100伏-240伏，频率50/60赫兹）。
注意：在使用前：-检查外接电源插座是好的，并且接口适合关节运动器的电源线接口。
该运动器的电源线符合现行的电源线标准并含有地线。
-插头必须接到符合标准的插座上。
-插座必须配有地线。
primerstar 说明书
PrimeSTAR ® HSDNA Polymerase with GC Buffer使 用 说 明 书TaKaRa Code：DR044A●包装量： 250 U●制品说明PrimeSTAR ® HS DNA Polymerase with GC Buffer适用于高保真扩增具有复杂二级结构的DNA模板（GC rich、重复序列等）。
PrimeSTAR ® HS DNA Polymerase具有极强的3’→5’Exonuclease活性，显示出超群的校正功能，同时还具有优于Taq DNA Polymerase的高扩增效率。
本制品对cDNA克隆等要求高保真的PCR反应能够发挥强大威力,并能对高GC含量模板进行高效扩增。
使用本制品扩增得到的PCR产物几乎都为平滑末端，经磷酸化后可直接克隆于具有平滑末端的载体中。
● 制品内容* Mg 2+浓度为2 mM。
●保 存 -20℃。
●活性定义用活性化的大马哈鱼精子DNA 作为模板/引物，在74℃，30分钟内，摄入10 nmol 的全核苷酸为酸性不溶物的活性定义为1个活性单位（U）。
●纯 度1）10 U 的本酶和0.6 μg 的λDNA-Hin d III 在74℃下反应1小时，DNA 的电泳谱带不发生变化。
2）10 U 的本酶和0.6 μg 的SupercoiledpBR322 DNA 在74℃下反应1小时，DNA 的电泳谱带不发生变化。
●用 途PCR 法高保真扩增DNA 片段。
●PCR 反应性能1） 以λDNA 为模板，可以很好地扩增8、10、12、15 kbp 的DNA 片段。
2） 以人基因组DNA 为模板，可以很好地扩增APO E 基因520 bp（GC 含量74.8%）的DNA 片段。
●反应条件 1. 按下列组份配制PCR 反应液。
2×PrimeSTAR ® GC BufferdNTP Mixture（2.5 mM each）Primer 1 Primer 2Template*PrimeSTAR ® HS DNA Polymerase（2.5 U/μl） dH 2O*【50 μl PCR 反应体系中模板DNA 推荐使用量】人基因组DNA 大肠杆菌基因组DNA λDNA 质粒DNA cDNA Library25 μl4 μl 0.2-0.3 μM （final conc.） 0.2-0.3 μM（final conc.）<200 ng0.5 μlPrimeSTAR ® HS DNA Polymerase（2.5 U / μl） 2×PrimeSTAR ® GC Buffer (Mg 2+plus)* dNTP Mixture（2.5 mM each） 100 μl 1.7 ml×3 800 μl 2. PCR 反应条件*。
Super Star 使用说明书(中)
Super Star CFM系列数字微波产品使用手册北京地杰通信设备股份有限公司2002年11月第一章绪论 (3)1.1 Super Star CFM系统的组成 (3)1.2 Super Star CFM的主要特点 (3)1.3 Super Star CFM无线系统的典型应用 (3)1.4 单端和站点配置 (3)1.5 Super Star CFM微波产品在现代市场的地位 (4)1.6 技术术语缩写 (4)第二章室内单元 (6)2.1 室内单元(IDU)概述 (6)2.2 IDU的类型 (6)2.3 IDU功能设计 (8)第三章室外单元 (10)3.1 概述 (10)3.2 Super Star CFM的ODU的特点和子段分配 (10)3.3 国内数字微波接力通信系统部分频段的射频配置方案 (12)3.4 ODU（射频单元）功能的设计 (13)第四章安装与开通 (16)4.1 IDU的安装 (16)4.2 IDU的前后面板 (16)4.3 IDU使用的电缆 (18)4.4 ODU接口及标识 (18)4.5 天线 (19)4.6 天线与ODU的连接 (20)4.7 无线链路计算 (21)4.8 天线的调节 (22)4.9 接地与防雷 (23)第五章管理系统 (24)5.1 概述及主要目标 (24)5.2 用液晶显示屏/按键对Super Star CFM设备管理配置的方法 (25)5.2.1液晶显示屏/按键 (25)5.2.2 LCD操作流程 (26)5.2.3 IDU LCD的“状态显示”（“Status Display”）模式 (27)5.2.4“设置”（“Setup”）模式时IDU的 LCD (28)5.3 用RS-232串行管理端口对Super Star CFM设备管理配置的方法 (29)5.3.1 RS-232串行管理端口 (29)5.3.2 用于Telnet或超级终端方式的命令行 (30)5.3.3 使用Telnet或超级终端方式的概述 (32)5.3.4 使用超级终端或Telnet方式的界面 (33)5.4 用Web的方式对Super Star CFM设备管理配置的方法 (37)5.5 ODU参数设定 (40)5.5.1 缺省射频设置 (40)5.5.2 设定Tx频率 (40)5.5.3 Tx功率设定 (40)5.6 缺省设置 (41)5.7 更新IDU的管理软件的方法 (42)第六章环回测试与故障判断 (45)6.1 利用环回测试的功能判断故障 (45)6.2 从IDU LCD/按键设置环回 (46)6.3 从Telnet/ASCII终端设置环回 (47)6.4 更换IDU (47)6.5 重启功能 (47)第七章技术数据 (48)7.1 主要参数 (48)7.2 机械数据 (48)7.3 供电 (49)7.4 管脚定义 (50)7.5 环境要求 (51)第八章 2E1全室外型ODU (52)8.1 2E1 FULL ODU 概述 (52)8.1.1 技术参数 (52)8.1.2 综合电缆的要求 (53)8.1.3 ODU的标识 (54)8.2 管理接口 (54)8.2.1 RS-232串行端口 (54)8.2.2 ASCⅡ控制台的命令行接口（CLI） (54)8.3﹑配置设备参数 (55)8.3.1﹑ODU的缺省设置 (55)8.3.2﹑配置发信频率 (55)8.3.3 频率波道的分配 (56)8.3.4 配置发信功率 (58)8.4 AGC端口 (58)8.5 环回测试 (58)8.5.1 射频环回 (58)8.5.2 基带环回 (58)8.5.3 E1接口环回 (58)8.5.4 通过ASCⅡ控制台的命令行接口（CLI）设置环回 (59)8.6 针脚 (60)8.7 电源 (60)第1章第一章绪论Super Star CFM系列数字微波系统在视距的条件下通过微波传输数字信号。
ZEISS Primostar HD配置指南说明书
May 2014All AirPorts and Apple TVs should be configured AHEAD of the demo – need AirPort Utility (for Windows or for Mac - /kb/dl1547 or check iTunes) and iTunes (https:///itunes/ ). You WILL need some sort of internet connection for both (more so for Apple TV). Most institutions have some sort of wifi network available. Furthermore, some businesses may have Apple IDs you can use in order to download and install the app on the iPad(s). You might not want to configure customer’s iPads using your own email address as an Apple ID, but for demos, it should be fine.To set up ZEISS Primostar HD and ZEISS Labscope1) Set up wireless router (AirPort – white) – connect white power cable to the back and to anoutlet.2) Set up ZEISS Primostar HD microscope – use the attached power supply to power themicroscope body. For the camera binocular head, attach the supplied “Y” USB cable to the back of the head and to the included wall charger adapter (only one end of the “Y” needed for this,when used in stand-alone mode, ie. not connected to a PC). The GREEN light on the side of the camera head should turn light up, assuming power is available. Attach the Ethernet cable from back of camera head to the back of the AirPort (not to the WAN port).You can add up to three microscopes directly to an AirPort. If you need to connect more microscopes, consider adding an unmanaged switch and connecting that the microscopes to that (and the switch to the router).NOTE: Labscope supports, in general, an unlimited number of microscopes. When there are more microscopes in the network than fit on one page in the Live tab, another page (andMay 2014another, etc.) will be added. They can be accessed by swiping left or right.The AirPort router (or any SOHO router for that matter) will have a hard time serving more than 20 live streams wirelessly though, because it will run out of bandwidth on the WLAN channel. Therefore for larger installations, multiple WLAN access points (could be AirPorts, but other 802.11n/ac access points will also work) will be needed, interconnected via LAN.3) Open Airport Utility on your computer (the iPad App does not detect unconfigured routers). Scanfor the AirPort (probably called “Base Station xxx”). If you have multiple routers to connect, click on the first one, configure it, and then repeat the process for the other routers later. (Thoserouters might need to be configured as Extenders – not covered here.) Click on the correctrouter and hit Continue.4) Name the wireless router. In this example, I called it “Carl Zeiss – Primostar HD router” and ifyou need to, add a number at the end, so you can tell all the routers apart. The password forALL router configurations should be “zeiss” (no quotations – case sensitive). Hit Continue.May 20145) Click I want to create a wireless network and Continue.6) Call the wireless network “Carl Zeiss”. Choose “no security” and specifically click Ignore in thewindow that pops up after hitting Continue.May 20147) Disable the guest network.8) Select I am not ready to connect to the Internet right now (unless you are, but thisconfiguration is for a stand-alone system).9) Click Update and the device will restart.10) When looking for DNS Servers, simply click Continue. You will get a warning. You MUST hitIgnore in order for the status light on the AirPort to turn GREEN and for the connections to ultimately work.11) Click Update again.May 201412) Setup is now complete. You should be returned to the home screen which lists all availableAirPorts in the area. You can close the AirPort Utility at this point.13) Set up the iPad – If you DO have a wireless network available to you – Start the iPad (notconnected to a computer). Choose a language and a country. When prompted to connect to an existing WiFi network – if you have an existing WiFi network, you may connect to it for theconfiguration to proceed. Disable Location Services. Select “Set up as new iPad.” Whenprompted for an Apple ID – you can skip this step. Agree to the Terms of Service. You maycreate a passcode for the iPad at this time, or can do so later. Don’t use Siri. Don’t senddiagnostics. Click Get Started to reach the Home screen. Open the App Store and search for“Labscope” to download it. You will be prompted for an Apple ID. You may use your own Apple ID, or you may create a fake one (although you’ll need a real email address to receive theverification email). You can associate a maximum of 10 iPads with this Apple ID, so it is likely that one fake account should cover your needs (you could use both your old and new ZEISSemail addresses, for instance, to add the app to 20 iPads). If you set up the iPad in this manner, simply install Labscope directly from the iPad.14) Set up the iPad - If you DO NOT have a wireless network available to you – Start the iPad.On the iPad, select a language and country. Select “Connect via iTunes” when prompted for a network connection. The iPad will prompt you to connect the included USB charger cable to the iPad and a computer that has iTunes installed. iTunes and the iPad will load automatically. InMay 2014iTunes, select “Set up as new iPad” and click Continue. The iPad will now progress to Location Services. Disable Location Services. Select “Set up as new iPad.” When prompted for an Apple ID – you can skip this step. Agree to the Terms of Service. You may create a passcode for the iPad at this time, or can do so later. Don’t use Siri. Don’t send diagnostics. Click Get Started to reach the Home screen. From the iTunes Store, search for and download Labscope. You will be prompted for an Apple ID. You may use your own Apple ID, or you may create a fake one(although you’ll need a real email address to receive the verification email). You can associate a maximum of 10 iPads with this Apple ID, so it is likely that one fake account should cover your needs (you could use both your old and new ZEISS email addresses, for instance, to add theapp to 20 iPads). If you set up the iPad in this manner, switch to the Apps tab in iTunes andclick Install next to Labscope, then click Apply at the bottom of the window. iTunes will then sync with the iPad and install the application.15) On the iPad, open the Settings. Click WiFi and locate the “Carl Zeiss” network that you created.Click on that network to connect to it, and type in a password as necessary. Return to the Home screen and start Labscope.16) On the overview tab (top tab, left side), you will see two Virtual Microscopes and eventually, youshould see Primostar HDs. Initially, each one may have a strange number associated with it.17) Click on the gear, located in the lower-right corner of each microscope, to configure thatmicroscope. On the left side of the window, you will be able to choose if this setup is forPrimostar HD or AxioCam ERc 5s Rev. 2 (compound or stereo). For “Microscope name,” youmay call the microscope whatever you want, or along any naming convention. For directidentification, you might want to name the microscope by the Serial Number on the back of the microscope body, so there can be no confusion which microscope you are receiving imagesfrom. Configure other information about the setup as appropriate. You may lock thisconfiguration, if desired.May 2014To connect ZEISS Primostar HD directly to a computer1) Connect the provided “Y” USB cable to the back of the camera head. Connect BOTH ends atthe opposite end of the cable to the computer. If DVD 52 is installed already, the driver should automatically install, as with other USB devices. In Device Manager, the camera will show up as such:2) Open ZEN (lite, Pro). The camera will show under the Active Camera dropdown as an“AxioCam ERc5s”.Operating Notes•NOTE: The HD head CANNOT be connected to an iPad and to a computer simultaneously.•NOTE: SD Card must be formatted to FAT32 prior to recording images.•NOTE: SD Card can be read by computer if HD head is connected to a PC. (It might prompt you to format the SD Card – DO NOT DO THIS. The images are available in the removable drivethat now shows up in Computer.) Images can no longer be captured to the SD Card; they must now be captured using ZEN.•NOTE: SD Card and AirPort connection are possible simultaneously. Labscope will simply showa “Snap Delay” screen. If you remove the SD Card in the middle of shooting, you must closeand re-open Labscope app. If you re-insert the SD Card, you must close Labscope via double-tapping the Home button (from the Home screen on the iPad). Re-insert the SD Card and re-open the app. Images snapped from Labscope will be saved to the iPad; images snapped via the “Snap” button the camera head will be saved to the SD Card.。
PrimeSTAR HS DNA Polymerase
PrimeSTAR ® HS DNA Polymerase使 用 说 明 书TaKaRa Code：DR010A●制品说明PrimeSTAR ® HS DNA Polymerase是兼具高保真性和高扩增效率的PCR用DNA聚合酶。
因其具有极强的3’→5’Exonuclease活性而显示出超群的校正功能，同时还具有优于Taq DNA Polymerase的高扩增效率。
制品中添加了在常温状态下能够抑制DNA Polymerase活性及3’→5’Exonuclease活性的抗体，有效防止PCR反应前的引物错配和引物消化。
与最适反应Buffer配合使用，可以实现对广泛靶序列的高保真性、高灵敏度、高特异性、高成功率的扩增，对于cDNA克隆等要求高保真的PCR反应发挥最强大的威力。
扩增得到的PCR产物几乎都为平滑末端，可直接克隆于平滑末端的载体中。
●制品内容（250 U） 30分钟内，摄入10 nmol 的全核苷酸为酸性不溶物的活性定义为1个活性单位（U）。
●纯 度1）10 U 的本酶和0.6 μg 的λ-Hin d III 在74℃下反应1小时，DNA 的电泳谱带不发生变化。
2）10 U 的本酶和0.6 μg 的Supercoiled pBR322DNA 在74℃下反应1小时，DNA 的电泳谱带不发生变化。
●用 途PCR 法扩增DNA 片段。
●PCR 反应性能1） 以λDNA 为模板，可以很好地扩增8、10、12、15 kbp 的DNA 片段。
2） 以人基因组DNA 为模板，可以很好地扩增0.5、1、2、4、6、8 kbp 的DNA 片段。
2. PCR 反应条件设置如下： ① 3 Step 法98℃ 10 sec 68℃ 4 min3. PCR 扩增结果。
【以人基因组DNA 为模板扩增0.5、1、2、4、6、8 kbp 的DNA 片段】●注意事项1. PCR 的反应液的配制可以在室温下进行，但DNA 聚合酶等各种试剂在配制前请于冰上放置。
Super_Star_-_IP_产品说明书-24E1
DG TELECOM
Super Star–ⅠP 系列
PDH 数字微波通信设备 用户手册
北京地杰通信设备股份有限公司
版本：2.8.1
Super Star–ⅠP 系列数字微波通信设备用户手册
目录
第一章 系统描述...........................................................................................................................3 1.1 关于本手册.........................................................................................................................3 1.2 绪论....................................................................................................................................3 1.3 系统特性............................................................................................................................3 1.4 系统组成............................................................................................................................4 1.5 缩略语................................................................................................................................6
Premix-PrimeSTAR-HS-使用说明书
Premix溶液组成
PrimeSTAR® HS DNA聚合酶 dNTP Mixture PrimeSTAR® Buffer
1.25 U/25 μl 2×conc.；各 0.4 mM 2×conc.；2 mM Mg2+
保存温度: -20℃。反复冻融活性可能降低，请在第一次融解后轻轻混匀，然后 分装至 PCR 反应管中（50 μl 反应时，每管分装 25 μl）后-20℃保 存，以避免多次反复冻融。
30 Cycles
注）本酶具有高退火效率，所以在设定 PCR 条件时可以缩短退 火时间。
应用例 1. 以人基因组DNA为模板进行PCR扩增。
模 板： 人基因组DNA 50 ng（50 μl反应体系）。
PCR仪： TaKaRa PCR Thermal Cycler Dice®。
PCR条件：
【0.5～6 kb时进行3 Step PCR】
25 μl <200 ng 0.2-0.3 μM（final conc.） 0.2-0.3 μM （final conc.） up to 50 μl
*【50 μl PCR 反应体系中模板 DNA 推荐使用量】
人基因组 DNA 大肠杆菌基因组 DNA λ DNA 质粒 DNA cDNA Library
PCR结果： M12345M
1 : 2 kb 的 DNA 扩增片段 2 : 4 kb 的 DNA 扩增片段 3 : 6 kb 的 DNA 扩增片段 4 : 8 kb 的 DNA 扩增片段 5 : 10 kb 的 DNA 扩增片段 M : λ-Hind III digest
注意事项 1. 为保持制品活性，请在第一次融解后轻轻混匀，分装至 PCR 反
2. 以大肠杆菌基因组DNA为模板进行PCR扩增。
PrimeSTAR Max DNA Polymerase For Research Use 说明书
Cat. #R045AProduct ManualPrimeSTAR® Max DNAPolymeraseFor Research Usev201510DaTable of ContentsI. Description (3)II. Components (3)III. Storage (3)IV. General Composition of PCR Reaction Mixture (3)V. PCR Conditions (4)VI. Optimization of Parameters (6)VII. Features (7)VIII. Electrophoresis, Cloning, and Sequencing of Amplified Products (12)IX. Troubleshooting (12)X. Related Products (13)I. DescriptionPrimeSTAR Max DNA Polymerase is a unique high-performance DNA polymerase that possesses the fastest extension speed available, along with the extremely high accuracy, high sensitivity, high specificity, and high fidelity of PrimeSTAR HS DNA Polymerase. High priming efficiency and extension efficiency greatly reduces the time required for annealing and extension steps, facilitating exceptionally fast high-speed PCR reactions. In addition, standardization of extension step time makes PrimeSTAR Max DNA Polymerase suitable for reactions with large amounts of template DNA that would ordinarily be difficult to amplify. Furthermore, an antibody-mediated hot start formulation prevents false initiation events during the reaction assembly due to mispriming and primer digestion. Since PrimeSTAR Max DNA Polymerase is configured as a 2-fold premix containing reaction buffer and dNTP mixture, it allows quick preparation of reactions and is useful for high-throughput applications.II. Components (for 100 reactions, 50 μl volume)PrimeSTAR Max Premix (2X) 625 μl x 4＊ Containing 2 mM Mg2+ and 0.4 mM each dNTPIII. Storage–20℃Note: Repeated freeze-thaw of the Premix may reduce its activity.IV. General Composition of PCR Reaction MixtureFinal conc.PrimeSTAR Max Premix (2X)25 μl1XPrimer 110 - 15 pmol0.2 - 0.3 μMPrimer 210 - 15 pmol0.2 - 0.3 μMTemplate< 200 ng＊Sterile distilled water to reaction volume of 50 μl＊: Refer to VI. Optimization of ParametersCaution:The PCR reaction mixture can be prepared at room temperature. However,keep each of the reaction components on ice during the preparation process.V. PCR ConditionsWhen performing rapid amplification protocols using PrimeSTAR Max DNAPolymerase, 3-step reactions are recommended for best results and longestamplification products.(A) For reactions in which the quantity of template is 200 ng / 50 μl or less:＊98℃10 sec.55℃ 5 sec. or 15 sec.30 - 35 cycles72℃ 5 sec./kb(B) For reactions in which the quantity of template exceeds 200 ng / 50 μl:＊98℃10 sec.55℃ 5 sec. or 15 sec.30 - 35 cycles [3-step PCR]72℃30 - 60 sec./kbor98℃10 sec.30 - 35 cycles [2-step PCR]68℃30 - 60 sec./kb＊: For rapid amplification protocols (extension step of 5 to 10 sec./kb) withcDNA as template, use a quantity of template that is equal to or less thanthe equivalent of 125 ng of total RNA / 50 μl reaction.If larger quantites of cDNA template are desired, by setting a longerextension time (up to 1 min./kb), it is possible to use up to the equivalentof 750 ng total RNA / 50 μl reaction.See VII.C. Template Quantity and Reaction Speed Using cDNA as Template.・ Denaturing conditions: An initial denaturation step is not necessary for some PCRenzymes, including the PrimeSTAR polymerase series;98℃ for 10 seconds is sufficient for complete denaturation.During cycling, denaturation at 98℃ for 5 to 10 sec. isrecommended.Denaturation at 94℃ is also possible, but the time shouldbe extended to 10 to 15 sec.・ Annealing temperature: Use 55℃ as the default annealing temperature.・ Annealing time: For primers that are 25-mer or shorter:For primer T m values (calculated by the formula below) of55℃ or greater, anneal for 5 sec.For primer T m values (calculated by the formula below)less than 55℃, anneal for 15 sec.For primers longer than 25-mers:Use an annealing time of 5 sec.＊Tm value calculation:Tm (℃) = 2(NA + NT) + 4(NC + NG) - 5where N represents the number of primer nucleotides having thespecified identity (A, T, C, or G)・ Final elongation: This step is typically recommended for Taq polymerase, but isnot always necessary with PrimeSTAR Max polymerase.Important note:Because the priming efficiency of PrimeSTAR Max DNA Polymerase is extremely high, use an annealing time of 5 sec. or 15 sec. Longer annealing times may cause smearing of PCR products visible during electrophoresis analysis.If smearing occurs when performing a 3-step PCR protocol, try a 2-step PCR protocol. See VI. Optimization of Parameters and IX. Troubleshooting.VI. Optimization of ParametersIn order to obtain the best PCR results, it is important to optimize the PrimeSTAR Max DNAPolymerase reaction parameters to fully utilize the enzyme's properties and advantages.(1) Template DNARecommended quantities of template DNA (50 μl reaction) for rapid amplificationprotocols (extension step of 5 sec./kb):Human genomic DNA 5 ng - 200 ngE. coli genomic DNA 100 pg - 200 ngλDNA 10 pg - 10 ngPlasmid DNA 10 pg - 1 ngWhen using more than 200 ng of DNA as template in a 50 μl reaction, use anextension time of 30 to 60 sec./kb for best results.For rapid amplification protocols (extension time of 5 to 10 sec./kb) with cDNA astemplate, set the template cDNA quantity to ≦the equivalent of 25 to 125 ng total RNAper 50 μl reaction.See VI. C. Template Quantity and Reaction Speed Using cDNA as Template.Do not use templates containing uracil, such as bisulfite-treated DNA.(2) Amplified Product SizesAmplification product sizes using an extension time of 5 sec./kb (for genomic DNAtemplates) or 5 to 10 sec./kb (for cDNA templates):Human genomic DNA up to 6 kbE. coli genomic DNA up to 10 kbcDNA up to 6 kbλDNA up to 15 kbWhen amplifying targets in excess of these lengths, try using an extension time of 15to 30 sec./kb. In such instances, amplification is affected by the quantity, quality, andsequence composition of the template.(3) Primer and PCR ConditionsSelect primer sequences using primer design software such as OLIGO Primer AnalysisSoftware (Molecular Biology Insights, Inc.).For general amplification, 20 to 25-mer primers are suitable. When amplifying longerproducts, the use of 25 to 30-mer primers may improve results. See section V. PCRConditions.Do not use inosine-containing primers with PrimeSTAR Max DNA Polymerase.(4) Annealing conditionsSelect annealing conditions as described in V. PCR Conditions. If low product yieldoccurs, try the following:<If smearing and/or extra bands appear on agarose electrophoresis gels>(1) Shorten the annealing time. If performing at 15 sec., set to 5 sec.(2) If the annealing step has already been set to 5 sec., raise the annealingtemperature to 58℃ - 63℃.(3) Perform 2-step PCR.<If the target product is not amplified>(1) Lengthen the annealing time. If performing at 5 sec., set to 15 sec.(2) Lower the annealing temperature to 50℃ - 53℃.Good amplification is observed for products up to 6 kb in length using an extension time of 10 sec. and for products up to 8 kb in length using an extension time of 30 sec.When λDNA is used as template, extension time of 5 sec./kb may be suitable.(2) With human genomic DNA as template, amplification of products ranging in sizefrom 0.5 kb to 7.5 kb was performed using an annealing time of 5 sec. and an extension time of either 10 or 30 sec.Template Human genomic DNA [100 ng / 50 μl reaction]Thermal cyclerTaKaRa PCR Thermal Cycler Dice(Not available in all geographic locations. Check for availability in your region.)PCR conditions98℃10 sec.55℃ 5 sec.30 cycles72℃10 or 30 sec.M: λ-Hin d III digest[Extension time: 30 sec.]M1246810M (kb)8M1246(kb)10M [Extension time: 10 sec.]VII. FeaturesA. Rapid Amplification(1) With λDNA as template, amplification of products ranging in size from 1 to 10 kbwas performed using an annealing time of 5 sec. and an extension time of either 10 or 30 sec.Template λDNA [1 ng/50 μl reaction]Thermal cyclerTaKaRa PCR Thermal Cycler Dice™(Not available in all geographic locations. Check for availability in your region.)PCR conditions98℃10 sec.55℃ 5 sec.30 cycles72℃10 or 30 sec.M: λ-Hin d III digest[Extension time: 10 sec.]M 0.5123467.5M(kb)[Extension time: 30 sec.]M 0.5123467.5M (kb)Good amplification is observed for products up to 4 kb in length using an extension time of 10 sec, and for products up to 6 kb in length using an extension time of 30 sec. With human genomic DNA as template, an extension time setting of 5 sec./kb may be suitable.(3) With cDNA template, amplification of products ranging in size from 1 kb to 6 kb wasperformed using an annealing time of 15 sec. and an extension time of either 10 or 30 sec.Template cDNA [equivalent to 100 ng total RNA) / 50 μl reaction]Thermal cyclerTaKaRa PCR Thermal Cycler Dice(Not available in all geographic locations. Check for availability in your region.)PCR conditions98℃10 sec.55℃15 sec.30 cycles72℃10 or 30 sec.M: λ-Hind III digest[Extension time]M 1246M 1246M (kb)10 sec.30 sec.Good amplification was observed for products up to 2 kb in length using anextension time of 10 sec. and for products up to 4 kb using an extension time of 30 sec. With cDNA template, an extension time of 5 to 10 sec./kb is required.B. Length of amplification productsWith λDNA, E. coli genomic DNA, human genomic DNA, or cDNA as the template, amplification sizes of various DNA fragments were examined using an annealing time of 5 sec. or 15 sec. and an extension time of 5 sec./kb (genomic DNA) or 10 sec./kb (cDNA).Template:λDNA 1 ng E. coli genomic DNA 50 ng Human genomic DNA 100 ng cDNA equivalent to 100 ng total RNAThermal cycler:TaKaRa PCR Thermal Cycler Dice PCR conditions: 98℃10 sec.55℃ 5 or 15 sec.30 cycles 72℃ 5 (or 10) sec./kbGood amplification of products up to 6 kb in length was observed using an extension time of 5 sec./kb.[ Human genomic DNA ]M 246M1(kb)0.57.53M: λ-Hin d III digestGood amplification of products up to 6 kb in length was observed using an extension time of 10 sec./kb.[ cDNA ][λDNA ]Good amplification of products up to 15 kb inlength was observed using an extension time of 5 sec./kb.M: λ-Hin d III digestM2468101215M 1(kb)Good amplification of products up to 10 kb in length was observed using an extension time of 5 sec./kb.[ E. coli genomic DNA ]M246810M: λ-Hind III digestM (kb)M2468M 1（kb）M: λ-Hind III digestC. Template quantity and reaction rate using cDNA as templateAmplification of transferrin receptor (TFR) 4 kb in length was performed with cDNA as template. cDNA was obtained by reverse transcription of various amounts of total RNA, as indicated. The extension times were set to 20 sec (5 sec./kb), 2 min (30 sec./kb) or 4 min (1 min./kb), and the amplification efficiencies were compared.Template quantity (50 μl reaction)1 : cDNA equivalent to 25 ng total RNA 2 : cDNA equivalent to 50 ng total RNA 3 : cDNA equivalent to 125 ng total RNA 4 : cDNA equivalent to 250 ng total RNAM 1234567M 1234567M 1234567M5 : cDNA equivalent to 500 ng total RNA6 : cDNA equivalent to 750 ng total RNA7 : cDNA equivalent to 1 μg total RNA M : λ-Hind III digest1 min./kb5 sec./kb30 sec./kbM : λ-Hind III digestM 1234M 1234M 1234M 1234MHumangenomic DNA E. coli genomic DNAλDNAPlasmidTemplate quantity*:Lane 1 Lane 2 Lane 3 Lane 4Human genomic DNA 100 pg 1 ng 10 ng 100 ng E. coli genomic DNA 1 pg 10 pg 100 pg 1 ng λDNA100 fg 1 pg 10 pg 100 pg Plasmid DNA100 fg1 pg10 pg100 pgFor rapid amplification protocols using an extension time of 5 sec./kb, it is necessaryto use cDNA template that is ≦ the equivalent of 125 ng total RNA / 50 μl reaction. When using longer extension times (up to 1 min./kb), the quantity of cDNA template can be increased up to the equivalent of 750 ng total RNA / 50 μl reaction.D. SensitivityWith various amounts of human genomic DNA, E. coli genomic DNA, λDNA, orplasmid DNA as template, sensitivity was examined when amplification of a 4 kb DNA fragment was performed using an extension time of 20 sec.Thermal cycler TaKaRa PCR Thermal Cycler Dice PCR conditions98℃55℃72℃10 sec.5 sec.20 sec.30 cycles *: Observed limit of detection indicated by underline.E. AccuracyThe fidelity of PrimeSTAR Max DNA Polymerase was examined by analysis of sequenc-ing data.[ Method ] Eight arbitrarily selected GC-rich regions were amplified with PrimeSTARMax DNA Polymerase or other DNA polymerases, using Thermus ther-mophilus HB8 genomic DNA as template.PCR products (approx. 500 bp each) were each cloned into a suitable plas-mid. Multiple clones were selected per respective amplification productand were subjected to sequence analysis.[ Result ] Sequence analysis of DNA fragments amplified using PrimeSTAR Max DNAPolymerase demonstrated only 9 mismatched bases per 230,129 total bases. This is higher fidelity than an alternative high-fidelity enzyme from Company A, and 10-fold higher fidelity than Taq DNA polymerase.＊: Out of 230,129 analyzed bases that were amplified using PrimeSTARMax DNA Polymerase, only 9 base errors occurred.Fidelity comparison of each enzymem u t a t i o n f r e q u e n c y （%）0.060%0.050%0.040%0.030%0.020%0.010%0.000%PrimeSTARHSPrimeSTAR Max ＊Company A High FidelityEnzymePfuTaqVIII．Electrophoresis, Cloning, and Sequencing of Amplified Products1) ElectrophoresisTAE Buffer is recommended for agarose gel electrophoresis of amplified products that areobtained using PrimeSTAR Max DNA Polymerase.Note : Use of TBE Buffer may result in DNA band patterns that are enlarged at the bottom of the gel.2) Termini of amplified productsMost PCR products amplified with PrimeSTAR Max DNA Polymerase have blunt-endtermini. Accordingly, they can be cloned directly into blunt-end vectors. If necessary,phosphorylate the amplified products before cloning. Use of Mighty Cloning Reagent Set(Blunt End) (Cat. #6027)is recommended for cloning into a blunt-end vector.3) Restriction enzyme reactionPrior to performing restriction enzyme digestion of amplified PCR products, remove alltraces of PrimeSTAR Max DNA Polymerase from the reaction mixture by phenol/chloro-form extraction or by using NucleoSpin Gel and PCR Clean-up (Cat. #740609.10/.50/.250).Particularly for 3'-protruding restriction enzymes such as Pst I, the 3'-protruding terminiproduced by these enzymes may be deleted by 3' → 5' exonuclease activity of PrimeSTARMax DNA Polymerase, if residual polymerase remains present in the restriction digest reaction.4) Direct sequencingPerform phenol/chloroform extraction of PCR products prior to direct sequencing toensure inactivation of 3' → 5' exonuclease activity. Alternatively, NucleoSpin Gel and PCRClean-up (Cat. #740609.10/.50/.250) may be used to purify DNA prior to sequencing.IX. TroubleshootingEvent Possible causes ActionNo amplification orpoor amplification efficiency Extension time Set to 10 to 60 sec./kb *Number of cycles Set to 35 to 40 cycles.Annealing time Set to 15 sec.Annealing temperature Lower by 2℃ per trialReaction volume Use 25 μl.Purity and quantity oftemplate DNAUse an appropriate amount of template DNA.Purify the template DNA＊.Primer concentration Use 0.2 - 0.5 μM (final conc.).Electrophoresis analysis shows smeared band(s) or extra band(s)Annealing time Set to 5 sec.AnnealingtemperatureRaise by 2℃ per trial up to 63℃.Try 2-step PCR.Template DNAquantityUse an appropriate amount of template DNA.Do not use more than necessary.Number of cycles Set to 25 to 30 cycles.Primer concentration Use at a final concentartion of 0.2 - 0.3 μM.＊: When using crude samples containing large quantities of RNA, such as samples prepared by thermal lysis, improved results may be achieved by setting the extension time to60 sec./kb.X. Related ProductsPrimeSTAR® HS DNA Polymerase（Cat. #R010A/B）PrimeSTAR® HS (Premix) (Cat. #R040A)PrimeSTAR® GXL DNA Polymerase (Cat. #R050A/B）PrimeSTAR® Mutagenesis Basal Kit (Cat. #R046A)＊NucleoSpin Gel and PCR Clean-up (Cat. #740609.10/.50/.250）Mighty Cloning Reagent Set (Blunt End) (Cat. #6027)TaKaRa PCR Thermal Cycler Dice™ Gradient/Standard (Cat. #TP600/TP650)＊TaKaRa PCR Thermal Cycler Dice™ Touch (Cat. #TP350)＊＊ : Not available in all geographic locations. Check for availability in your region.PrimeSTAR is a registered trademark of TAKARA BIO INC.Thermal Cycler Dice is a trademark of TAKARA BIO INC.NOTE :This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, orhousehold item, etc.Takara products may not be resold or transferred, modified for resale or transfer, or usedto manufacture commercial products without written approval from TAKARA BIO INC.If you require licenses for other use, please contact us by phone at +81 77 565 6973 orfrom our website at .Your use of this product is also subject to compliance with any applicable licensingrequirements described on the product web page. It is your responsibility to review,understand and adhere to any restrictions imposed by such statements.All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.。
PrimeSTAR HS DNA 聚合酶使用说明书
Cat. #R044AProduct ManualPrimeSTAR® HS DNA Polymerasewith GC BufferFor Research Usev201908DaTable of ContentsI. Description (3)II. Components (3)III. Storage (3)IV. General PCR Reaction Mixture (3)V. PCR Conditions (4)VI. Optimization of Parameters (5)VII. Fidelity (6)VIII. Amplification Examples (7)IX. Electrophoresis, Cloning, and Sequencing of Amplified Products .10 X. Troubleshooting (11)XI. Related Products (12)I．DescriptionPrimeSTAR HS DNA Polymerase is a unique high fidelity DNA polymerase that additionally offers high amplification efficiency for PCR amplification. PrimeSTAR HS possesses a robust 3’ →5’ exonuclease activity, resulting in superior proofreading activity and a low error rate. It also has high amplification efficiency (superior to that of Taq DNA polymerase). Furthermore, presence of a monoclonal antibody in the reaction mixture suppresses both the DNA polymerase and 3’ →5’ exonuclease activities prior to the first denaturing step, preventing false initiation events during reaction assembly due to mispriming and primer digestion. Finally, PrimeSTAR’s high priming efficiency makes it possible to shorten reaction times by reducing the length of the annealing step.PrimeSTAR HS DNA Polymerase with GC Buffer was developed for accurate amplification of GCrich targets. With PrimeSTAR GC Buffer, amplifications exhibit both the high fidelity and high amplification efficiency expected from PrimeSTAR HS DNA Polymerase, and additionally yield excellent results in high-specificity applications such as amplification of GC rich DNA templates.II．Components (for 200 reactions)PrimeSTAR HS DNA Polymerase (2.5 U/μl) 100 μl2X PrimeSTAR GC Buffer (Mg2+ plus)＊ 1.7 ml x 3dNTP Mixture (2.5 mM each) 800 μl＊Mg2+ concentration is 2 mM (2X)III．Storage- 20℃IV．General PCR Reaction Mixture (50 μl volume)Reagent Volume/Amount Final Conc.2X PrimeSTAR GC Buffer (Mg2+ plus) 25 μl1XdNTP Mixture (2.5 mM each) 4 μl200 μM eachPrimer 110 - 15 pmol0.2 - 0.3 μMPrimer 210 - 15 pmol0.2 - 0.3 μMTemplate DNA＜200 ngPrimeSTAR HS DNA Polymerase (2.5 U/μl)0.5 μl 1.25 U/50 μlSterile purified water up to 50 μl＊ T he PCR reaction mixture can be prepared at room temperature. However, theenzyme and other reagents should be kept on ice during operation.V．PCR ConditionsThis kit is designed to perform amplification of GC rich target DNA.Typically, best results are obtained using a 2-step PCR protocol. However, if thisprotocol does not yield sufficient product in quality and quantity, the 3-step PCRprotocol is recommended. Also, refer to the following sections : VI. Optimization ofParameters and X. Troubleshooting.(A) 2-Step PCR Protocol98℃68℃10 sec1 min/kb30 cycles(B) 3-Step PCR Protocol98℃60℃72℃10 sec5 sec1 min/kb30 cyclesNote:The PrimeSTAR HS has extremely high priming efficiency.Therefore, when using the 3-step PCR Protocol, the annealing time should be set at only 5 sec. Longer annealing times can result in increased background.VI．Optimization of ParametersPrimeSTAR HS DNA Polymerase with GC Buffer is designed to perform amplification ofGC rich targets, while exhibiting both the high accuracy and efficiency characteristicof PrimeSTAR HS DNA Polymerase. Optimization of parameters in PCR condition maybe required to generate maximum performance.(1) For best results, use of 1.25 U of enzyme per 50 μl reaction mixture is recommended.However, depending upon the size of the amplified fragment and the purity andamount of template used, the amount of enzyme may need to be modified. Forexample, if smearing or non-specific banding is observed, results may be improvedby reducing the amount of enzyme to ～0.625 U/50 μl.(2) Template DNARecommended template DNA amounts (assuming a 50 μl reaction)Human genomic DNA : 5 - 200 ngE. coli genomic DNA :100 pg- 100 ngcDNA : 1 - 200 ngλDNA :10 pg- 10 ngPlasmid DNA :10 pg- 1 ngNote :Avoid using excess amounts of template DNA. The efficiency of reactioncan be decrease, particularly when more than 200 ng of template is used.DNA, which contain uracil, cannot be used as a template.(3) dNTP and Mg2+ concentration:Because dNTPs have a chelative effect, higher dNTP concentrations lower theeffective Mg2+concentration of the reaction mixture. The supplied 2X PrimeSTARGC Buffer provides final concentration of 1 mM Mg2+ in the reaction mix, that hasbeen optimized for use with a 200 μM each dNTP concentration. Avoid modifyingthe dNTP concentration in the reaction mix.Furthermore, substitution of dTTP with dUTP in a PrimeSTAR HS DNA Polymerasereaction mix is also not recommended as amplification efficiency will be loweredsignificantly.(4) Primer and PCR conditionsCommercially available primer design software, such as OLIGO Primer AnalysisSoftware (Molecular Biology Insights), is recommended for determining appropriateprimer sequences. For general amplification reactions, 20 - 25 mer primers shouldyield satisfactory results.Avoid the use of primers that contain inosine with PrimeSTAR HS DNA Polymerase.However, it is possible to use degenerate primers with this enzyme.＊ Amplification with PrimeSTAR HS DNA Polymerase with GCBuffer results in only 25 errors out of a total 304,110 bases.Comparison of Enzyme FidelityPrimeSTAR HS PrimeSTAR HSwith GC BufferPfu Taqm u t a t i o n f r e q u e n c y (%)VII．FidelityAfter PCR amplification of eight randomly selected regions (each about 500 bp in length) of GC rich Thermus thermophilus HB8 genomic DNA, PCR products were cloned into a vector. Multiple clones were selected for each product and sequenced, and the mutation frequency was determined.In this assay, the fidelity of PrimeSTAR HS DNA Polymerase with GC Buffer was higher than that of Pfu DNA Polymerase, and was similar to those with PrimeSTAR HS DNA Polymerase (PrimeSTAR Buffer used).Sequence analysis is the most accurate method for obtaining enzyme fidelity comparisons relevant to the most commonly-used applications. These resultsdemonstrate that PrimeSTAR HS with GC Buffer is a reliable polymerase especially for high fidelity reaction.PrimeSTAR HS DNA Polymerase with GC Buffer provides excellent amplification of GC rich targets in comparison to the other enzymes.Template:1 : Tth HB8 genomic DNA 100 pg2 : Tth HB8 genomic DNA 1 ng3 : Tth HB8 genomic DNA 10 ng M : λ-Hin d III digest← 3005 bpTaq DNApol.PrimeSTAR HSPrimeSTAR HS with GC buffer 3 step 3 step 2 step 2 step M 123M 123M M123M 123M1233 step [ Tth HB8 3005 bp ]Template:1 : Human genomic DNA 1 ng2 : Human genomic DNA 10 ng3 : Human genomic DNA 100 ng M : 100 bp DNA Ladder← 520 bpTaq DNA pol.PrimeSTAR HS PrimeSTAR HS with GC buffer3 step 3 step 2 step2 step M 123M123M 123M 123M [ Human APOE gene 520 bp ]VIII．Amplification ExamplesResult 1 :The reactivity of Taq DNA Polymerase, PrimeSTAR HS DNA Polymerase, and PrimeSTAR HS DNA Polymerase with GC Buffer were compared for amplification of HumanAPOE gene (520 bp; 74.8% GC) and a region of Tth HB8 (3005 bp; 73.2% GC) as targets. The reaction mixture and PCR conditions were according to the recommended protocol of each product.[ Human APOE gene 746 bp ][ Human TGF β1 gene 2005 bp ]Result 2 :The reactivity of the GC rich high fidelity enzymes of Company A, Company B, and Company C were compared with PrimeSTAR HS DNA Polymerase with GC Buffer for amplification of Human APOE (746 bp; 73.9% GC), TGF β1 (2005 bp; 68.8% GC), and regions of Tth HB8 (3005 bp, 73.2% GC, and 5030 bp, 71.2% GC) as targets. Each enzyme was reacted at the recommended reaction mixture and PCR conditions.Template:1 : Human genomic DNA 1 ng2 : Human genomic DNA 10 ng3 : Human genomic DNA 100 ngM : 100 bp DNA LadderTemplate:1 : Human genomic DNA 1 ng2 : Human genomic DNA 10 ng3 : Human genomic DNA 100 ngM：λ-Hin d III digest746 bp →M M1Company A PrimeSTAR HS with GC buffer 23M M M 123123123Company B Company C2005 bp →M 123M1PrimeSTAR HS with GC buffer 23M 123M 123M Company A Company B Company C[ Tth HB8 3005 bp and 5030 bp ]The results show that PrimeSTAR HS DNA Polymerase with GC Buffer providesexcellent amplification efficiency with higher specificity than other supplier's GC-rich high fidelity enzymes.Template:1：Tth HB8 genomic DNA 100 pg 2：Tth HB8 genomic DNA 1 ng 3：Tth HB8 genomic DNA 10 ng M：λ-Hin d III digestTemplate:1：Tth HB8 genomic DNA 100 pg 2：Tth HB8 genomic DNA 1 ng 3：Tth HB8 genomic DNA 10 ng M：λ-Hin d III digestCompany C 5030 bp →M 123M1PrimeSTAR HS with GC buffer 23M 123M 123M Company ACompany B3005 bp →PrimeSTAR HS with GC buffer3 step 2 step M 123M 123M M 123M 123M123Company C Company A Company BIX．Electrophoresis, Cloning, and Sequencing of Amplified Products(1) Electrophoresis of amplified productsTAE Buffer is recommended for agarose gel electrophoresis of amplified productsthat are obtained using PrimeSTAR HS DNA Polymerase with GC Buffer. Use of TBEBuffer may result in DNA banding patterns which become broad at the gel bottom.(2) CloningMost products amplified with PrimeSTAR HS DNA Polymerase with GC Buffer haveblunt-end termini. They (if necessary, phosphorylate before cloning) can be cloneddirectly into blunt-ended vectors. The Mighty Cloning Reagent Set (Blunt End) (Cat.#6027) is recommended for cloning into blunt-ended vectors.(3) Restriction Enzyme DigestionPrior to performing restriction enzyme digestion of the PCR products with PrimeSTARHS DNA Polymerasewith GC Buffer all traces of the polymerase should be removed by phenol/chloroformextraction. In particular, the removal of the polymerase is important to digest withenzymes arising 3’ -protruding cleavage sites, such as Pst I as residual PrimeSTARHS DNA Polymerase 3’ →5’ exonuclease result in deletion of 3’-protruding region.(4) Direct sequencingPhenol/chloroform extraction of PCR products prior to direct sequencing isrecommended to ensure inactivation of PrimeSTAR’s 3’ →5’ exonuclease activity.[ Tth HB8 5030 bp ]1.25 U/50 μl0.625 U/50 μl M 123M M123X．Troubleshooting Problem : No or poor amplification.(1) Purity and amount of template DNA ⇒Use proper amount of template DNA. ⇒Increase purity of DNA. (2) Annealing/extension temperature ⇒Lower temperature in decrements of 2℃.⇒Perform using 3-step PCR method.(3) Concentration of Primer ⇒Test the final primer concentration in a range of 0.2 - 0.5 μM.(4) Annealing time ⇒Set the annealing time for 3-step PCR for 15 sec.Problem: Extra or smearing bands.(1) Enzyme amount ⇒Decrease enzyme concentration to ～0.625 U/50 μl reaction.(2) Amount of template DNA ⇒Use an appropriate amount of template DNA. Avoid excessive amounts of template DNA. (3) Annealing/extension temperature ⇒Raise the temperature in increments of 2℃(4) Primer Concentration ⇒Test the final concentration in the range of 0.2 - 0.3 μM.(5) Cycle Number ⇒Set at 25 - 30 cyclesNOTE : This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, or household item, etc.Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from Takara Bio Inc.If you require licenses for other use, please contact us by phone at +81 77 565 6972 or from our website at .Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements.All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.XI．Related Products PrimeSTAR® HS DNA Polymerase (Cat. #R010A/B)PrimeSTAR® HS Premix (Cat. #R040A) PrimeSTAR® Max DNA Polymerase (Cat. #R045A/B）PrimeSTAR® GXL DNA Polymerase (Cat. #R050A/B）Mighty Cloning Reagent Set (Blunt End) (Cat. #6027) PrimeSTAR is a registered trademark of Takara Bio Inc.。
PrimeTest 100 电源供电设备用户指南说明书
1Operating InstructionsBracken HillSouth West Industrial Estate Peterlee Co Durham SR8 2SW ENGLANDTel: +44(0)191 5863511 ****************.uk ******************.ukPart Number 344A550 Revision 1© 2006 Seaward Electronic LtdLimited Warranty & Limitation of Liability SEAWARD Electronic Limited guarantees this product to be free from defects in material and workmanship under normal use and service for a period of 1 year. The period of warranty will be effective at the day of delivery.(c) Copyright 2006All rights reserved. Nothing from this edition may be multiplied, or made public in any form or manner, either electronically, mechanically, by photocopying, recording, or in any manner, without prior written consent from SEAWARD Electronic Limited. This also applies to accompanying drawings and diagrams.Due to a policy of continuous development SEAWARD Electronic Limited reserves the right to alter the equipment specification and description outlined in this publication without prior notice and no part of this publication shall be deemed to be part of any contract for the equipment unless specifically referred to as an inclusion within such contract.2DECLARATION OF CONFORMITYAs the manufacturer of the apparatus listed, declare under our sole responsibility that the product:PRIMETEST 100To which this declaration relates are in conformity with the relevant clauses of the following standard: BS EN 61010-1:2001Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 1: General requirements.BS EN 61326:1998Electrical equipment for measurement, control and laboratory user-EMC Requirements Performance: The instrument operates withinspecification when used under theconditions in the above standardsEMC and Safety Standards.The product identified above conforms to the requirements of Council Directive 89/336/EEC and 73/23 EEC.Seaward Electronic Ltd is registered under BS EN ISO9001:2000 Certificate No: Q05356.3Contents1Important Information 5 2Introduction7 3Performing Tests9 3.1Checking a mains power outlet9 3.2Testing a Class I Appliance10 3.3Testing a Class II Appliance13 3.4Testing a mains cord14 3.5Testing an extension lead15 4Specification17 5Maintenance18 6Battery Check18 6.1Battery Replacement19 7Service and Calibration20451 Important InformationThese operating instructions are intended for the use of adequately trained personnel.The following symbols are used in these operating instructions and on the PrimeTest 100.Caution, risk of electric shock.Indicates instructions must be followed to avoid danger to persons.Caution, risk of danger. The operating instructions must be adhered to in order to avoid danger.Before use, ensure unit is clean and dry; visually inspect all leads, connectors, and case. Any damage or wear must be rectified prior to use.Standard AccessoriesPart Number PrimeTest 100 unit 344A910 Carry Case71G082 Black Test Lead 1m347A002 UK IEC mains cord 0.5m 300A002 Operating Instructions 344A550Optional AccessoriesPart NumberPATGuard Lite352A930 PATGuard Workabout336A910 NiMH Batteries and charger 339A950Full details and specifications can be found at or by calling Seaward Sales on 0191 5878724 / 0191 5878748.6Figure 1. PrimeTest 100 Front ViewFigure 2. PrimeTest 100 End View2 IntroductionThe PrimeTest 100 is a hand held battery powered unit suitable for carrying out electrical safety checks on:• Class I appliances• Class II appliances• IEC mains leads• Extension leads• Mains outlet wiringNumbers shown in circles e.g.for connecting the appliance under test.• 4mm socket on end panelfor mains cord testing.User InterfaceThe LCD display+Class II appliance test =Note: The PrimeTest 100 will automatically switch OFF after approximately 3 minutes if no keys are pressed. The auto switch-off is disabled during a power socket test.7Note: When a key is pressed to initiate a test sequence, the PrimeTest 100 will compare the type of appliance connected with the test sequence that has been selected, and where possible, will prompt the user if an inappropriate test has been selected.For example, if an IEC lead is connectedbetween the front panel mains socketand a Class Iappliance test is selected, the PrimeTest 100 will flash the Cord test enunciatorto indicate that the Cordtest893 Performing TestsPresskeys to switch on the PrimeTest 100. When the unit is ready the display will be as shownbelow.3.1 Checking a mains power outletConnect the IEC power cord to the PrimeTest 100socket10If there is a fault with the Neutral connection this isindicated by the display below.In the event of a test failure, disconnect the PrimeTest 100 from the supply and rectify the fault.Do not leave the PrimeTest 100 permanently connected to a mains supply.The auto switch-off function is disabled when the PrimeTest 100 is connected to a live mains socket. The unit will beep continually after 3 minutes to remind the user to disconnect from the mains socket.3.2 Testing a Class I Appliance• Visually inspect the appliance and mains cord forsigns of damage.• If the appliance passes a visual inspectionproceed with the electrical tests.• Plug the earth test leadinto the 4mm socket• Connect the earth test probe to an exposed metal part on the appliance.• If the Appliance under test has an ON/OFF switch, make sure it is in the ON position. •Press the Class I test key11• The test sequence is halted.•If the measured value is less than 20 ohms but greater than the factory set pass/fail threshold, the measured value is displayed and the unit indicates a fail result as shown below•If the measured value is less than the factory set pass/fail threshold, the measured value is displayed and the unit indicates a pass result, as shown below.• The unit will proceed with the Insulation and Leakage tests.Note: The power switch on the appliance under must be in the ON position to perform aninsulation test. If no appliance is detectedthe PrimeTest 100 will display thefollowing.• Check that the appliance power switch is in the ON position. The test will automatically proceed if the appliance power switch is placed in the ON position.• If the LO LOAd enunciator remains on the display, the load presented by the appliance may be too small for the PrimeTest 100 to detect. Inthis case, press the test key123.3 Testing a Class II Appliance• Visually inspect the appliance and mains cord for signs of damage.• If the appliance passes a visual inspection proceed with the electrical tests.• Plug the earth test leadinto the 4mm socket• Connect the earth test probe to an exposed metal part on the appliance.• If the Appliance under test has an ON/OFF switch, make sure it is in the ON position.• Press the Class II test keyto continue.• If the Insulation Resistance is greater than the factory set limit a tick is placed next to the Riso enunciator. Similarly, if the Leakage13current is less than the factory set limit a tick is placed next to the I LEAK enunciator.• The PASS enunciator is illuminated.3.4 Testing a mains cord• Visually inspect the mains cord and plug for signs of damage.• Check that the correct fuse is fitted.• If the cord passes a visual inspection proceed with the electrical tests.• Plug the mains cord under test into the IEC socket on the PrimeTest 100.• Press the cords test key1415to the Riso enunciator and the test sequence is halted.• If the Insulation Resistance is greater than the factory set limit a tick is placed next to the Riso enunciator.• The unit will proceed with the wiring test, checking the live and neutral conductors for short or open circuits or reversed connections. •If the wiring is correct a tick is placed next to the cord enunciator, the GOOd enunciator is illuminated and a PASS is indicated for the sequence.Note: If the tested cord has a wiring fault, one of the following enunciators will be illuminated in place of GOOd• OPE n indicates that either the live orneutral conductor is broken (open circuit) or the plug top fuse has blown• Short indicates that the live and neutral conductors are shorted together•C r OSS indicates that the live and neutral connections are crossed (live and neutral conductors reversed)3.5 Testing an extension lead• Visually inspect the mains cord and plug forsigns of damage.• Check that the correct fuse is fitted.• If the cord passes a visual inspection proceedwith the electrical tests.• Plug the supplied 0.5m red IEC lead into the IEC socketon the PrimeTest 100.• The extension lead can now be tested in the same manner as an IEC as described above. Note: The protective earth resistance will depend upon the length of the extension lead and the size (cross sectional area) of the cable. The measured value maybe be acceptable even if a cross is shown next to the R PE enunciator.Table 1: Approximate resistance of protective earth conductors.Cord size / current rating0.5mm2/(3A) 1.0 mm2/(10A) 1.25 mm2/(13A) length5m 0.20 0.10 0.1010m 0.40 0.20 0.2025m 1.00 0.50 0.40For further information on protective conductor resistance and testing of portable appliances can be found in the Code of Practise for In-service Inspection and Testing of Electrical Equipment published by the IEE.164 SpecificationEarth ContinuityAccuracy* ± (5% + 2 digits)Test current 200mA minimumTest voltage 9V nominalInsulation resistanceAccuracy ± (5% + 2 digits)Test voltage 500VTest current >1mA into 500kΩTest current <2mA into 2kΩLeakage CurrentAccuracy ± (5% + 2 digits)Test voltage 40V rms, 50Hz ACTest current <5mA into 2kΩCord TestEarth continuity, insulation resistance as above.Check for Live and Neutral open circuit, short circuit or reversed polarity.*When used with Seaward test lead, Part Number 347A002 Factory Set Pass/Fail limitsClass I Class II CordEarth0.2 ohms N/A 0.2 ohm ContinuityInsulation1.0Mohm2.0Mohm 2.0Mohm ResistanceLeakage 0.75mA 0.25mA N/A17Environmental ratingIP Rating IP40Operating temperature range 0°C to 40°C, without moisture condensation.Storage temperature range –25° to 65°.Note: Batteries should be removed prior to storage.Overvoltage category 300V CAT II5 MaintenanceClean only with a dry cloth; do not use solvents. Before use, ensure unit is clean and dry; visually inspect all leads, connectors, and case. Any damage or wear must be rectified to preserve user safety. Check the battery contacts and compartment are free of electrolytic contamination.Any contamination of the battery contacts or compartment should be cleaned with a dry cloth. Note: The PrimeTest 100 contains no user serviceable parts. If an Error warning should appear on the display please contact the manufacturer or an authorised Seaward Service Agent for advise.6 Battery CheckThe PrimeTest 100 is powered from a 6 AA cells which are checked before a test is performed. Whenthe battery voltage is low theenunciator isilluminated. The unit will continue to perform within specification for a limited number of tests, dependent upon the type of the batteries fitted.When the battery voltage reaches a level where the performance is affected the enunciator will flash and all test keys are disabled. The batteries must be replaced.186.1 Battery ReplacementBefore opening the PrimeTest 100 ensurethat all test leads are disconnected.• Switch off the unit by pressing and holdingkeys .• Disconnect the black test lead from 4mm test socket.• Disconnect the EUT mains cable from the EUT socket197 Service and CalibrationTo maintain the specified accuracy of the measurement results, the instrument must be recalibrated at regular intervals by either the manufacturer or an authorised Seaward Service Agent. We recommend a recalibration period of one year.For help or advise on Service and Calibration contact:Service DepartmentSeaward ElectronicBracken HillSouth West Industrial EstatePeterleeCo Durham SR8 2SWEnglandTel: 0191 5878739 / 0191 5878737Email: ******************.ukWeb: 20。
PrimeSTAR GXL DNA Polymerase安全技术说明书
修订日期 2021-02-09修订编号2安全技术说明书本安全技术说明书符合下列要求：JIS Z 7253:2019第1部分：化学品及企业标识产品名称 PrimeSTAR® GXL DNA Polymerase 产品代码 ST2309 注册登记号 无资料供应商信息应急咨询电话In case of emergency, call PERS (Professional Emergency Resource Services) 1-800-633-8253 (US) or 801-629-0667 (international). 化学品的推荐用途和限制用途 确定用途仅用于研究。
不用于诊断过程限制用途 无资料第2部分：危险性概述GHS 分类急性毒性 - 经口 不能分类 急性毒性 - 经皮不能分类 急性毒性 - 吸入(气体) 分类不适用 急性毒性 - 吸入(蒸气)不能分类 急性毒性 - 吸入(粉尘/烟雾) 类别4 皮肤腐蚀/刺激 不能分类 严重眼损伤/眼刺激 不能分类 呼吸致敏 不能分类 皮肤致敏不能分类 生殖细胞突变性 不能分类 致癌性 不能分类 生殖毒性不能分类影响哺乳或通过哺乳产生影响对哺乳没有影响或不会通过哺乳产生影响供应商日本：Takara Bio Inc.Nojihigashi 7-4-38,Kusatsu, Shiga 525-0058, Japan 电话：+81.77.565.6972 网址： 中国：宝生物医学技术(北京)有限公司 昌平区科学园路22号生命科学园 北京，102206电话：+86 10 8072 0980网址：特异性靶器官系统毒性(一次接触) 不能分类 特异性靶器官系统毒性(反复接触) 不能分类 吸入危害 不能分类 急性水生毒性 不能分类 慢性水生毒性 不能分类 臭氧 不能分类GHS标签元素信号词 警告危险说明 吸入有害预防措施• 避免吸入粉尘/烟/气体/烟雾/蒸气/喷雾• 只能在室外或通风良好之处使用事故响应• 不适用• 如误吸入：将受害人转移到空气新鲜处，保持呼吸舒适的休息姿势• 如感觉不适，呼叫解毒中心或医生 安全储存• 不适用 处置• 不适用 其他危害 无资料。
GREAT PulseSTAR iButton 信息钮识读器 说明书
PulseStar操作手册进原装口 产国地：美Videx, Inc. is an industry leader in the design and manufacturing of data collection and accecontrolproducts.注意：产品规格或其他如有变动，恕不另行通知PulseStar is a trademark of Videx, Inc. PulseStar是Videx公司的注册商标Copyright © 2000 by Videx, Inc.产品介绍感谢您购买Videx公司的PulseStar产品产品。
PulseStar是Videx公司的一款坚固持久、防水的iButton TM信息钮识读器。
当成功地读取信息钮后会无声地振动提示，从而可以在潮湿、多雨的环境及嘈杂或需要安静操作的场所应用自如。
PulseStar配有的金属铝制外壳及镍读头，可以防止撞击，跌落及水造成的损伤。
PulseStar产品特性：♦金属铝制外壳及镍材料读头♦防水、抗撞击及跌落♦兼容读取全系列iButton信息钮的ID♦可存贮5400条记录♦48KB内存♦红外通讯接口♦内置实时时钟♦由可更换锂电池供电♦LED指示灯♦带有出厂唯一编码图1：PulseStar数据采集器、下载器产品介绍系统包括1)PulseStar数据采集器PS-0482)PulseStar数据下载器（含电源）PSD-F003)iButton信息钮4)系统管理软件5)串口通信线电脑配置要求视窗98、Me、2000或XP128MB内存最小2G硬盘空间奔腾III处理器空余的串口CD-ROM产品介绍内存PulseStar数据采集器内存为48KB，可以存储5400条记录（包括iButton ID 时间和日期）而无需下载到电脑。
一旦PulseStar内存已满，在读取iButton时LED灯会快速地闪烁，而不是正常地振动。
内存内的记录不会因为掉电而丢失。
primerstar HS DNA polymerase
[Result]
Sequence analysis of DNA fragments amplified using PrimeSTAR HS
polymerase had only 12 erroneous nucleotides in 249,941 total nucleotides. This fidelity is higher than that obtained with a high fidelity enzyme from Company A, and it is 10 times higher than Taq DNA polymerase.
< Human DCLRE1A gene [2 kb] >
N : No Template 1 : human genomic DNA 2 : human genomic DNA 3 : human genomic DNA 4 : human genomic DNA M : λ-Hin d III digest
II. Components (for 200 reactions)
PrimeSTAR HS DNA Polymerase (2.5 units/μl).................................... 100 μl 5X PrimeSTAR Buffer (Mg2+ plus) ＊.........................................................2 x 1 ml dNTP Mixture (2.5 mM each)........................................................................ 800 μl
primestar说明书
primestar说明书注意事项：1. 本文为Primestar产品的说明书，旨在帮助用户正确使用该产品。
请仔细阅读本说明书并按照指引操作，以确保最佳使用效果。
2. 本说明书不包含任何与购买或售后服务相关的信息。
如需了解更多信息，请咨询售卖商或生产商。
1. 产品概述Primestar是一款高性能的智能家居系统，通过联网技术，提供全方位的家居自动化控制解决方案。
无论您身在何地，只需一台智能设备，便可轻松实现对房屋内的设备、灯光、安防系统等的控制。
2. 系统要求Primestar智能家居系统适用于以下操作系统：- iOS 9及以上版本- Android 6及以上版本请确保您的智能设备满足以上要求，并已下载并安装Primestar的官方应用程序。
3. 硬件配置Primestar智能家居系统包括以下硬件组件：- 中央控制器：Primestar中央控制器为系统的核心，负责连接和管理所有设备。
请将中央控制器接入您家庭的无线网络。
- 智能设备：Primestar兼容多种智能设备，包括插座、灯具、摄像头、传感器等。
请确保您购买的设备与Primestar系统兼容。
4. 系统安装a. 将中央控制器连接至电源并开机。
b. 在您的智能设备上下载并安装Primestar应用程序。
c. 打开Primestar应用程序，按照提示创建您的Primestar账户，并登录。
d. 在应用程序的设置界面中，按照指引完成系统连接设置。
请确保中央控制器已正确连接至家庭无线网络。
5. 设备添加通过Primestar应用程序，您可以轻松添加和管理智能设备。
具体步骤如下：a. 在应用程序界面选择“添加设备”。
b. 按照设备添加指引，将智能设备连接至Primestar系统。
具体操作方式请参考设备说明书。
c. 添加完成后，您可以在应用程序中查看和控制已添加的设备。
6. 定时任务设置Primestar允许用户根据需要设置定时任务，自动控制设备状态。
您可以按照以下步骤进行定时任务设置：a. 在应用程序界面选择“定时任务”。
PrimeSTAR
Code No. R045A 研究用PrimeSTAR® MaxDNA Polymerase说明书目录内容页码●制品说明 1 ●制品内容 1 ●保存 1 ● PCR反应液的配制 1 ● PCR反应条件 1 ●参数优化 2 ● PrimeSTAR Max DNA Polymerase的特点 3 ●扩增产物的Agarose Gel电泳、克隆和测序7 ● Troubleshooting 8 ●关联产品8●制品说明PrimeSTAR Max DNA Polymerase不仅兼备了PrimeSTAR HS DNA Polymerase所具有的高扩增效率、高灵敏度和高特异性功能，还是延伸速度很快、保真性能很高的PCR用DNA聚合酶。
制品本身具有的高退火效率和特别开发的延伸因子使引物的退火时间和延伸时间大幅缩短，实现了PCR反应的高速化。
同时，针对由于核酸含量较高而难以扩增的反应体系，也只需正常设定延伸时间即可进行扩增，大大简化了操作过程。
本制品是添加了在常温下能够抑制DNA Polymerase活性和3’→5’exonuclease活性的抗体的Hot Start型DNA聚合酶。
同时反应各组分已经配制成预混型的2X Premix，在常温下可以快速配制反应液。
●制品内容（100次量）PrimeSTAR Max Premix（2X） 625 μl × 4* Mg2＋浓度为2 mM（2X），dNTP浓度是各0.4 mM（2X）。
●保存：-20℃。
注意：反复冻融活性可能降低。
● PCR反应液的配制（Total 50 μl）请参照参数优化。
注意：PCR反应液的配制可在室温下进行，但DNA聚合酶等试剂在配制前请于冰上放置。
● PCR反应条件使用PrimeSTAR Max DNA Polymerase进行快速扩增反应时，为了发挥延伸因子的最大效率，建议使用3 Step PCR反应条件。
（A）DNA量为200 ng/50 μl以下的PCR反应体系*98℃10 sec55℃ 5 sec or 15 sec 30～35 Cycles72℃ 5 sec/kb（B）DNA量为200 ng/50 μl以上的PCR反应体系*98℃10 sec55℃ 5 sec or 15 sec 30～35 Cycles [3-step PCR]72℃30～60 sec/kbor98℃10 sec30～35 Cycles[2-step PCR]68℃30～60 sec/kb*以反转录反应产物为模板进行高速PCR反应时（延伸速度为5～10 sec/kb），在50 μl反应体系中，cDNA的使用量为相当于125 ng Total RNA以下。
斯特拉克器 Prime X X光床摆说明书
Prime X X-ray Stretcher
Standard features • Clearview Technology Platform • Thermoformed ABS base with extra
capacity storage tray • 318 Kg weight capacity • Oxygen bottle holder • Integrated transfer board • Power-washable design (1105X and 1115X only) • Four-wheel, center-locking brake system
The information presented is intended to demonstrate the breadth of Stryker product offerings. A healthcare professional must always refer to the package insert, product label and/or instructions for use before using any Stryker product.
Simple cassette positioning The dual-deck design allows 360° positioning of cassettes.
Electric lift makes raising the stretcher easy by simply stepping on the pedal.
Overall width Siderails up Siderails down (1105X and 1115X) Siderails down (1125X)
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100 μl PrimeSTAR ® HS DNA Polymerase
使 用 说 明 书
Takara Code : DR010A 包 装 量：250 Units 制品说明 PrimeSTAR ® HS DNA Polymerase 是兼具高保真性和高扩增效率的PCR 用DNA 聚合酶。
因其具有极强的3’→5’Exonuclease 活性而显示出超群的校正功能，同时还具有优于Taq DNA Polymerase 的高扩增效率。
制品中添加了在常温状态下能够抑制DNA Polymerase 活性及3’→5’Exonuclease 活性的抗体，有效防止PCR 反应前的引物错配和引物消化。
与最适反应Buffer 配合使用，可以实现对广泛靶序列的高保真性、高灵敏度、高特异性、高成功率的扩增，对于cDNA 克隆等要求高保真的PCR 反应发挥最强大的威力。
扩增得到的PCR 产物几乎都为平滑末端，可直接克隆于平滑末端的载体中。
制品内容（250 U ）
PrimeSTAR ® HS
DNA Polymerase （2.5 U/μl ） 5×PrimeSTAR ® Buffer （Mg 2+ plus ）* 1 ml×2 dNTP Mixture （各2.5 mM ） 800 μl
* Mg 2+浓度（5×）为5 mM 。
保 存 : -20℃
dNTP Mixture
浓度 各2.5 mM 状态 水溶液（Na 盐，pH7.0～9.0） 纯度 各98%以上
* dATP 、dGTP 、dCTP 、dTTP 的等摩尔混合物， 不用稀释可直接用于PCR 反应。
活性定义 用活性化的大马哈鱼精子DNA 作为模板/引物，在74℃，30分钟内，
摄入10 nmol 的全核苷酸为酸性不溶物的活性定义为1个活性单位（U ）。
纯 度
1） 10 U 的本酶和0.6 μg 的λ-Hin d III 在74℃下反应1小时，DNA
的电泳谱带不发生变化。
2） 10 U 的本酶和0.6 μg 的Supercoiled pBR322 DNA 在74℃下
反应1小时，DNA 的电泳谱带不发生变化。
用 途
PCR 法扩增DNA 片段。
PCR 反应性能 1） 以λ DNA 为模板，可以很好地扩增8、10、12、15 kb 的DNA 片段。
2） 以人基因组DNA 为模板，可以很好地扩增0.5、1、2、4、6、8 kb 的DNA 片段。
实验操作程序 1. 按下列组份配制PCR 反应液。
5×PrimeSTAR ® Buffer （Mg 2+ plus ） 10 μl dNTP Mixture （各2.5 mM ） 4 μl Primer 1（10 μM ） 1 μl Primer 2（10 μM ） 1 μl 模板DNA* ＜200 ng PrimeSTAR ® HS DNA Polymerase （2.5 U/μl ） 0.5 μl 灭菌蒸馏水 up to 50 μl *【
50 μl PCR 反应体系中模板DNA 推荐使用量】
人基因组DNA 5 ng~200 ng （<200 ng ） 大肠杆菌基因组DNA 100 pg~100 ng λ DNA 10 pg~10 ng 质粒DNA 10 pg~1 ng 2. PCR 反应条件设置如下： ① 3 Step 法 98℃ 10 sec 55℃ 5 sec 或15 sec 30 Cycles 72℃ 1 min/kb
② 2 Step 法 98℃ 10 sec 68℃ 1 min/kb
* 本酶具有高退火效率，所以在设定PCR 条件时可以缩短退火时间。
应用例 【以λ DNA 为模板扩增8、10、12、15 kb 的DNA 片段】
1. 按下列组份配制PCR 反应液。
5×PrimeSTAR ® Buffer （Mg 2+ plus ） 10 μl dNTP Mixture （各2.5 mM ） 4 μl Primer 1（10 μM ） 1 μl Primer 2（10 μM ） 1 μl λDNA （10 pg/μl ） 1 μl PrimeSTAR ® HS DNA Polymerase （2.5 U/μl ） 0.5 μl 灭菌蒸馏水 up to 50 μl 2. PCR 反应条件。
98℃ 10 sec.
68℃ 4 min.
30 Cycles 30 Cycles
3. PCR 扩增结果。
【以人基因组DNA 为模板扩增0.5、1、2、4、6、8 kb 的DNA 片段】 1. 按下列组份配制PCR 反应液。
5×PrimeSTAR ® Buffer （Mg 2+ plus ） 10 μl dNTP Mixture （各2.5 mM
）
4 μl Primer 1（10 μM ） 1 μl Primer 2（10 μM ） 1 μl 人基因组DNA （100 ng/μl ） 1 μl PrimeSTAR ® HS
DNA Polymerase （2.5 U/μl ） 0.5 μl 灭菌蒸馏水 up to 50 μl
2. PCR 反应条件。
98℃ 10 sec.
68℃ 8 min.
3. PCR 扩增结果。
1. PCR 的反应液的配制可以在室温下进行，但DNA 聚合酶等各种
试剂在配制前请于冰上放置。
2. 因本酶具有高退火效率，使用本酶进行三步法PCR 反应时，退
火时间不宜过长，只需设定5 sec 或15 sec （常规为30 sec ）。
NOTICE TO PURCHASER: LIMITED LICENSE
[M54] PrimeSTAR ® HS DNA Polymerase
This product is covered by the claims of U.S. Patent No. 7,704,713 and its foreign counterparts
[L15 ] Hot Start PCR
Licensed under U.S. Patent No. 5.338,671 and 5,587,287, and corresponding patents in other countries.
V2012.02
30 Cycles。