大鼠ELISA试剂盒说明书,AECAELISA试剂盒

大鼠ELISA试剂盒说明书，AECAELISA试剂盒
大鼠ELISA试剂盒说明书，AECAELISA试剂盒
产品规格：96T/48T
供应商：上海樊克生物有限公司
本产品只用于科研实验
大鼠ELISA试剂盒说明书，AECAELISA试剂盒，小鼠(LR/Ob-R)ELISA试剂盒，苗条素受
体elisakit elisa试剂盒上海樊克生物供应！
大鼠ELISA试剂盒说明书，AECAELISA试剂盒Prospective application: ELISA method for quantitative determination of human serum and plasma,
Content of related substances in cell culture supernatant or other related biological fluids.
1 Kit preservation: -20 C (a longer time to use); 2-8 C (frequent use).
2 washing liquid preserved at low temperature there will be precipitation, heating the water to help dissolve when diluted.
3 Chinese and English instructions may be inconsistent, please refer to the specification in english.
4 just open the enzyme linked plate hole may contain a little water samples, this is
a normal phenomenon, will not have any effect on the results of the experiment.
7 could not detect the NaN3 containing samples, because NaN3 inhibited the activity of horseradish peroxidase (HRP).
大鼠ELISA试剂盒说明书，AECAELISA试剂盒Experimental conditions of ELIAS kit:
1 solid carrier selection: many substances can be used as solid carrier, such as polyvinyl chloride, polystyrene, polypropylene amide and cellulose, etc.. The form can be a concave plate, test tube, beads, etc..
2 pack is the choice of antibodies or antigens: the antibody or antigen adsorbed on the surface of the solid carrier, the requirements are better, the general requirements of PH in 9 ~ 9.6 between.
3 the choice of the working concentration of enzyme labeled antibody: first of all, using the direct ELISA method to carry on the preliminary titer titration. And then fixed the other conditions or take the "square method", in the formal experimental system to accurately titration its work concentration.
4 the substrate of the enzyme and the choice of the donor: the choice of hydrogen donor is cheap, safe, and has obvious color reaction. Some of the hydrogen donor has the potential carcinogenic effect, ELIAS kit should pay
国产/进口Elisa试剂盒，96T/48TElisa试剂盒， 96T/48Telisakit价格
大鼠ELISA试剂盒说明书，AECAELISA试剂盒Elisa kit test rules:
1, to ensure the accuracy of the gun, the error can not be more than 2%. Available water and electronic balances are determined. But it's better to have professional personnel to correct it.
2, to be equipped with 20ul, 50ul, 100ul, 1000ul and a volley. Draw different liquids, to replace the gun head. Even when the standard is drawn.
1, 3 hours before the experiment to remove the kit from the refrigerator, so that a variety of reagents are restored to room temperature, in order to make the results more stable.
4, the experiment, to make the substrate to avoid light storage.
5, with the gun to draw the liquid speed can not be too fast, so as not to produce bubbles and to absorb the amount is not accurate.
6, when the liquid, to use the range and the need to close the gun to suck, reduce the error.
7, when the liquid is added to the enzyme standard hole, the liquid contact of the liquid drop and the hole wall of the gun head can be avoided by avoiding the contact of the liquid drop and the hole wall in the gun head.
8, after all the liquid added, the enzyme labeled plate on the table in parallel to gently shake 30 seconds, mixed with liquid. Can also use the shaking function of the enzyme standard instrument.
9, should try to do two experiments, so as to ensure the accuracy of the data. 10, the results have questions about the sample to be confirmed by other methods.
大鼠ELISA试剂盒说明书，AECAELISA试剂盒Kit performance
1 sensitivity: the minimum detection concentration is less than 1 standard. Linearity of dilution. Sample linear regression and the expected concentration correlation coefficient R value is 0.990.
2: no specific reaction with other cytokines.
3 repeatability: plate, plate between the coefficients of variation were less than 10%.
The result of judgment and analysis
1, the instrument value: Yu Bo 450nm ELISA od read the hole on the instrument
2, to the OD value for the longitudinal axis (y), corresponding HLA-B27 standard concentration as the abscissa (x), do the corresponding curve, HLA-B27 content in the sample can be according to its OD value by standard curve conversion out corresponding concentration.
3, the detection range: 0-8.0IU/ml
4, sensitivity: 0.01 IU/ml
上海樊克生物有限公司只用于科研实验等领域科学研究，不得用于临床诊断。