小鼠尿微量白蛋白ALB说明书
小鼠尿微量白蛋白(ALB)说明书
小鼠尿微量白蛋白(ALB）酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定小鼠血清，血浆,相关液体样本尿微量白蛋白(ALB）含量。
实验原理：本试剂盒应用双抗体夹心法测定标本中小鼠尿微量蛋白(ALB)水平。
用纯化的小鼠尿微量蛋白(ALB)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入尿微量蛋白(ALB)，再与HRP标记的ALB抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的尿微量蛋白(ALB)呈正相关。
用酶标仪在450nm波长下测定吸光度（OD 值），通过标准曲线计算样品中小鼠尿微量蛋白(ALB)浓度。
试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×48 1×96 2-8℃保存标准品：540μg/L 0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液 1.5ml×1瓶 1.5ml×1瓶2-8℃保存酶标试剂 3 ml×1瓶 6 ml×1瓶2-8℃保存样品稀释液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂A液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液 3 ml×1瓶 6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如出现沉淀，应再次离心。
ALB
白蛋白（ALB）测定操作规程（SOP文件）1实验原理和检验目的本试剂按WHO推荐的溴甲酚绿(BCG)法配制。
原理如下：在酸性溶液( pH4.15)中白蛋白与BCG形成绿色络合物，其色泽与白蛋白含量成正比，对照标准可测定其含量。
2标本采集和处理2.1标本种类和采集方法标本：血清，采血后应及时分离，避免溶血，血清在2~8℃稳定3天。
受检者（体检对象或病人）的准备：对于体检对象抽血前应有2周时间保持平时的饮食习惯。
静脉采血：除非是卧床的病人，一般在采血时取坐位。
体位影响水分在血管内外的分布。
2.2标本处理方法标本要放在带盖的容器内以防污染和蒸发。
样品采集后要离心标本，吸出血清。
标本应立即测定。
处理样品时要将其当成生物污染品。
3试剂3.1试剂组成3.2试剂准备试剂为液体单试剂试剂，开瓶即可使用，用后及时置于2～8℃避光保存。
3.3试剂稳定性试剂和标准液在2-8℃避光保存可稳定12个月；试剂开口稳定。
4校准4.1校准品每日进行试剂空白校准操作。
使用朗道（RONDOX）进行全点校准操作。
4.2校准品准备（RONDOX）校准物复溶30分钟，充分溶解混匀后即可使用。
4.3校准品稳定性（RONDOX）冻干校准物2～8℃密闭保存稳定至有效期，复溶后校准物15℃～25℃稳定8小时，2℃～8℃稳定2天，-15℃～-25℃稳定2周。
4.4试剂校准周期推荐校准周期为2天。
当试剂批号更换时应重新校准。
5质控5.1质控品朗道（RONDOX）血清进行室内质控。
5.2可接受性判断质控物的检测值应在给定质控范围，或可以通过参加卫生部室间质评对实验室的运作情况进行系统评估。
5.3质控操作每日进行样本检测之前首先应进行质控操作，以考察系统的在控情况。
如果检测结果符合质控要求则进行样本操作；如果不符合质控要求，则应重复质控操作，以排除可能发生的偶然误差；如果仍不符合质控要求，则应考虑质控品的重新准备、试剂的重新校准或更新、仪器的维护等。
建议在样本的检测过程中和/或结束后再次进行质控操作，以考察样本的检测全过程的在控情况。
尿微量白蛋白(ALB)测定试剂盒(酶联免疫吸附法)产品技术要求普恩光德
尿微量白蛋白（ALB）测定试剂盒（酶联免疫吸附法）适用范围：本试剂盒用于体外定量测定人尿液样本中微量白蛋白(ALB)浓度。
1.1 包装规格48人份、96人份1.2 主要组成成分质控品质控范围批特异，详见标签。
2.1 外观2.1.1 试剂盒各组分应齐全、完整，液体无渗漏。
2.1.2 标识应清晰，易识别。
2.2 空白检出限空白检出限浓度应不高于2.0ng/ml。
2.3 线性在[0.4，51.2]mg/L范围内线性相关系数r≥0.9900。
2.4 重复性分别用高、低浓度的样品各重复检测10次（样品浓度范围20mg/L±4.0mg/L和12.8mg/L±3.0 mg/L），其变异系数（CV）应不大于10%。
2.5 准确度用中国食品药品检定研究院的人血清白蛋白标准品（270009）对试剂盒进行测试，相对偏差（B）应不超过±20%。
2.6 分析特异性按表1所示交叉反应物规定浓度进行测定，检测结果的浓度值不得超过4.0mg/L。
表1：交叉反应物及浓度列表2.7 溯源性根据GB/T21415-2008《体外诊断医疗器械生物样品中量的测量校准品和控制物质赋值的计量学溯源性》及有关规定提供校准品的来源、赋值过程及测量不确定度等内容。
校准品溯源至企业工作校准品，并与已上市产品比对赋值。
2.8 质控品赋值有效性本试剂盒质控品的测定结果应在质控范围内。
2.9 批间差用3个批号试剂盒检测同一份样品（样品浓度范围20mg/L±4.0mg/L），则三个批号试剂盒之间的批间变异系数CV(%)应不超于15%。
2.10 稳定性将试剂盒各组分置2℃～8℃放置，有效期为12个月，效期后两个月内，检定结果应符合2.1、2.2、2.3、2.4、2.5、2.6的规定。
cusabio 微量白蛋白尿(MAU ALB)检测试剂盒使用说明书
Human microalbunminuria(MAU/ALB) ELISA kit Catalog Number. CSB-E08970hFor the quantitative determination of human microalbunminuria(MAU/ALB) concentrations in serum, plasma, urine.This package insert must be read in its entirety before using this product.If You Have ProblemsTechnical Service Contact informationPhone: 86-27-87582341Fax: 86-27-87196150Email:****************Web: In order to obtain higher efficiency service, please ready to supply the lot numberof the kit to us (found on the outside of the box).1PRINCIPLE OF THE ASSAYThis assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for MAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated MAU. A competitive inhibition reaction is launched between MAU (Standards or samples) and biotin-conjugated MAU with the pre-coated MAU antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of MAU bound in the initial step. The color development is stopped and the intensity of the color is measured.DETECTION RANGE0.078 µg/ml-5 µg/ml.SENSITIVITYThe minimum detectable dose of human MAU is typically less than 0.019 µg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human MAU concentration that could be differentiated from zero.SPECIFICITYThis assay has high sensitivity and excellent specificity for detection of human MAU. No significant cross-reactivity or interference between human MAU and analogues was observed.Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human MAU and all the analogues, therefore, cross reaction may still exist.2PRECISIONIntra-assay Precision (Precision within an assay): CV%<8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays):CV%<10%Three samples of known concentration were tested in twenty assays to assess.LIMITATIONS OF THE PROCEDUREFOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.The kit should not be used beyond the expiration date on the kit label.Do not mix or substitute reagents with those from other lots or sources.If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding.This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.3MATERIALS PROVIDEDReagents QuantityAssay plate (12 x 8 coated Microwells) 1(96 wells) Standard (Freeze dried) 2Biotin-conjugate (100 x concentrate) 1 x 60 µlHRP-avidin (100 x concentrate) 1 x 120 µlBiotin-conjugate Diluent 1 x 10 mlHRP-avidin Diluent 1 x 20 ml Sample Diluent 2 x 20 mlWash Buffer (25 x concentrate) 1 x 20 mlTMB Substrate 1 x 10 mlStop Solution 1 x 10 ml Adhesive Strip (For 96 wells) 4Instruction manual 1STORAGEUnopenedkitStore at 2 - 8°C. Do not use the kit beyond the expiration date.Opened kitCoated assayplateMay be stored for up to 1 month at 2 - 8°C.Try to keep it in a sealed aluminum foil bag,and avoid the damp.Standard May be stored for up to 1 month at 2 - 8° C.If don’t make recent use, better keep it storeat -20°C.HRP-avidinBiotin-conjugateBiotin-conjugateDiluentMay be stored for up to 1 month at 2 - 8°C. HRP-avidinDiluentSample DiluentWash BufferTMB SubstrateStop Solution*Provided this is within the expiration date of the kit.4OTHER SUPPLIES REQUIREDMicroplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.An incubator which can provide stable incubation conditions up to 37°C±0.5°C.Squirt bottle, manifold dispenser, or automated microplate washer.Absorbent paper for blotting the microtiter plate.100ml and 500ml graduated cylinders.Deionized or distilled water.Pipettes and pipette tips.Test tubes for dilution.PRECAUTIONSThe Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.5SAMPLE COLLECTION AND STORAGESerum Use a serum separator tube (SST) and allow samples to clot for30 minutes before centrifugation for 15 minutes at 1000 x g, 2 - 8°C.Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Centrifuge the sample again after thawing before the assay.Plasma Collect plasma using EDTA, or heparin as an anticoagulant.Centrifuge for 15 minutes at 1000 x g, 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Centrifuge the sample again after thawing before the assay.Urine Use a sterile container to collect urine samples. Remove any particulates by centrifugation for 15 minutes at 1000xg, 2 - 8°C and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Centrifuge again before assaying to remove any additional precipitates that may appear after storage.SAMPLE PREPARATIONRecommend to dilute the serum or plasma samples 50000-fold before test.The suggested 50000-fold dilution can be achieved by adding 2µl sample to 398µl of normal saline. Complete the 50000-fold dilution by adding 2µl of this solution to 498µl of Sample Diluent. The recommended dilution factor is for reference only. The optimal dilution factor should be determined by users according to their particular experiments.Recommend to dilute the urine samples with Sample Diluent(1:40) before test. The suggested 40-fold dilution can be achieved by adding 6µl sample to 234µl of Sample Diluent. The recommended dilution factor is for reference only. The optimal dilution factor should be determined by users according to their particular experiments6Note:1. CUSABIO is only responsible for the kit itself, but not for the samplesconsumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.2. Samples to be used within 5 days may be stored at 2-8°C, otherwisesamples must be stored at -20°C (≤1month) or -80°C (≤2month) to avoid loss of bioactivity and contamination.3. Grossly hemolyzed samples are not suitable for use in this assay.4. If the samples are not indicated in the manual, a preliminary experiment todetermine the validity of the kit is necessary.5. Please predict the concentration before assaying. If values for these arenot within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.6. Tissue or cell extraction samples prepared by chemical lysis buffer maycause unexpected ELISA results due to the impacts of certain chemicals.7. Owing to the possibility of mismatching between antigen from otherresource and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.8. Influenced by the factors including cell viability, cell number and alsosampling time, samples from cell culture supernatant may not be detected by the kit.9. Fresh samples without long time storage are recommended for the test.Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.7REAGENT PREPARATIONNote:Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit. Bring all reagents to room temperature (18-25°C) before use for 30min.Prepare fresh standard for each assay. Use within 4 hours and discard after use.Making serial dilution in the wells directly is not permitted.Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved.To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µl for once pipetting.Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.1. Biotin-conjugate (1x) - Centrifuge the vial before opening.Biotin-conjugate requires a 100-fold dilution. A suggested 100-fold dilution is 10 µl of Biotin-conjugate + 990 µl of Biotin-conjugate Diluent.2. HRP-avidin (1x) - Centrifuge the vial before opening.HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 µl of HRP-avidin + 990 µl of HRP-avidin Diluent.3. Wash Buffer(1x)- If crystals have formed in the concentrate, warm up toroom temperature and mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 ml of Wash Buffer (1 x).894.StandardCentrifuge the standard vial at 6000-10000rpm for 30s.Reconstitute the Standard with 1.0 ml of Sample Diluent . Do not substitute other diluents. This reconstitution produces a stock solution of 5 µg/ml. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.Pipette 150 µl of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (5 µg/ml). Sample Diluent serves as the zero standard (0 µg/ml).Tube S7 S6 S5S4 S3 S2 S1 S0 µg/ml52.51.250.6250.3120.1560.078ASSAY PROCEDUREBring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay.It is recommended that all samples and standards be assayed in duplicate.1. Prepare all reagents, working standards, and samples as directed in theprevious sections.2. Refer to the Assay Layout Sheet to determine the number of wells to beused and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.3. Set a Blank well without any solution.4. Add 50µl of standard and sample per well.5. Add 50µl of Biotin-conjugate(1x) to each well(not to Blank well). Coverwith a new adhesive strip. Incubate for 60 minutes at 37°C.(Biotin-conjugate(1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)6. Aspirate each well and wash, repeating the process two times for a total ofthree washes. Wash by filling each well with Wash Buffer (200µl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.7. Add 100µl of HRP-avidin(1x) to each well(not to Blank well). Cover themicrotiter plate with a new adhesive strip. Incubate for 60 minutes at 37°C.8. Repeat the aspiration/wash process for five times as in step 6.9. Add 90µl of TMB Substrate to each well. Incubate for 20 minutes at 37°C.Protect from light.10. Add 50µl of Stop Solution to each well, gently tap the plate to ensurethorough mixing.1011. Determine the optical density of each well within 5 minutes, using amicroplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.*Samples may require dilution. Please refer to Sample Preparation section. Note:1. The final experimental results will be closely related to validity of theproducts, operation skills of the end users and the experimental environments.2. Samples or reagents addition: Please use the freshly prepared Standard.Please carefully add samples to wells and mix gently to avoid foaming. Do not touch the well wall as possible. For each step in the procedure, total dispensing time for addition of reagents or samples to the assay plate should not exceed 10 minutes. This will ensure equal elapsed time for each pipetting step, without interruption. Duplication of all standards and specimens, although not required, is recommended. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions.Also, use separate reservoirs for each reagent.3. Incubation: To ensure accurate results, proper adhesion of plate sealersduring incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. Once reagents have been added to the well strips, DO NOT let the strips DRY at any time during the assay. Incubation time and temperature must be observed.4. Washing: The wash procedure is critical. Complete removal of liquid ateach step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting and remove any drop of water and fingerprint on the bottom of the plate. Insufficient washing will result in poor precision and falsely elevated absorbance reading. When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.115. Controlling of reaction time: Observe the change of color after adding TMBSubstrate (e.g. observation once every 10 minutes), TMB Substrate should change from colorless or light blue to gradations of blue. If the color is too deep, add Stop Solution in advance to avoid excessively strong reaction which will result in inaccurate absorbance reading.6. TMB Substrate is easily contaminated. TMB Substrate should remaincolorless or light blue until added to the plate. Please protect it from light.7. Stop Solution should be added to the plate in the same order as the TMBSubstrate. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the TMB Substrate.1213ASSAY PROCEDURE SUMMARY*Samples may require dilution. Please refer to Sample Preparation section.CALCULATION OF RESULTSUsing the professional soft "Curve Expert" to make a standard curve is recommended, which can be downloaded from our web.Average the duplicate readings for each standard and sample and subtract the average optical density of Blank.Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the MAU concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.14人尿微量白蛋白(MAU/ALB)酶联免疫试剂盒使用说明书【产品编号】CSB-E08970h【预期应用】ELISA法定量测定人血清、血浆、尿液中MAU含量。
白蛋白(ALB)检测标准操作规程
深圳迈瑞生物医疗电子股份有限公司白蛋白测定方法
2、适用范围
适用于血清白蛋白的测定
3、试剂仪器
3.1试剂：深圳迈瑞生物医疗电子股份有限公司原装试剂盒
3.2未开启的试剂盒避光保存于2℃—8℃有效期一年。
试剂不可以冰冻。
4、操作程序
4.1方法原理
溴甲酚绿（B CG）在P H4.2环境中，有非离子型表面活性剂存在时，可与白蛋白形成蓝绿色复合物，颜色的深度与样本中白蛋白浓度成正比。
4.2样本要求
新鲜血清或肝素血浆，样本收集后应尽快测定，必须避光保存，避免使用溶血样本。
4.3上机操作
4.3.1试剂装载、校准、样品和质控血清分析操作详见“《迈瑞BS-220全自动生化分析仪标准操作、维护、保养规程》”
4.3.2校准“
4.3.2.1 标准液的准备：校准品使用深圳迈瑞生物医疗电子股份有限公司配套冻干品，按说明书要求稀释后分装，-20℃冷冻保存，用前提前15分钟从冰箱取出，复溶到室温后上机检测。
4.3.2.2校准程序：每30天需要定标一次。
当有以下情况时需重新定标：
1）换试剂批号或出现质控漂移时；
2）当仪器做完保养后；
3）仪器进行零件更换时。
每次试验前用准备好的校准品进行定标，定标通过后进行检测。
艾美得白蛋白(ALB)测定试剂盒（溴甲酚绿法）说明书
白蛋白(ALB)测定试剂盒（溴甲酚绿法）说明书【产品名称】白蛋白(ALB)测定试剂盒（溴甲酚绿法）【包装规格】a)单一试剂：4×40mLb)单一试剂：5×60mLc)单一试剂：2×100mL【预期用途】用于体外定量测定人体血清中白蛋白的含量。
白蛋白是主要的血清蛋白，主要在肝脏合成，是衡量肝合成功能的一个重要指标。
由于白蛋白独特的分子结构使它成为多种物质如胆红素、脂肪酸、尿酸以及各种药物和抗体的运输载体，此外，白蛋白还具有维持机体渗透压的功能。
白蛋白升高主要见于脱水，降低主要见于营养不良、肝脏疾病、肾功能紊乱和风湿性关节炎等[1]。
【检验原理】在рH为4.2的缓冲液中，白蛋白分子带正电荷，与带负电荷的溴甲酚绿（BCG）生成蓝绿色复合物，在630nm处有吸收峰，复合物的吸收光与白蛋白浓度成正相关。
【主要组成成分】试剂主要组分磷酸氢二钠-柠檬酸缓冲液210mmol/L溴甲酚绿（BCG）0.25mmol/L单十二烷基九乙二醇醚适量注：不同批号试剂盒中各组分未经试验不可互换。
【储存条件及有效期】贮存于2～8℃，有效期为18个月，生产日期、有效期见标签。
【适用仪器】艾威德AS-420/AS-660/AS-1200；日立HITACHI7020型/7060型/7180型/7600型/LABOSPECT008AS型；贝克曼AU400/AU480/AU640/AU680/ AU2700/AU5400/AU5800/AU5811/AU5821；佳能TBA-FX8/TBA-120FR/ TBA-2000FR；罗氏cobas8000c702/cobas8000c701/cobas8000c502；西门子SIEMENS ADVIA1800/ADVIA2400；雅培ABBOTT ARCHITECT c8000/ARCHITECT c16000/ARCHITECT ci8200；西森美康SYSMEX BM6010/C；科华KHB卓越310/卓越330/卓越400/卓越450/ZY-1200/ZY-1280；迪瑞CS-240/CS-T300/CS-300B/CS-380/CS-400A/CS-400B/CS-600A/ CS-600B/CS-800A/CS-800B/CS-1200/CS-1200ISE/CS-1300B/CS-1400；迈瑞MINDRAY BS-220/BS-330/BS-350E/BS-380/BS-390/BS-400/BS-430/BS-600/ BS-800/BS-2000M；颐兰贝ES-200/ES-380/ES-480；赛诺迈德SUNMATIK-9050型；雷杜Chemray420；英诺华D280；特康TC6010L；锦瑞GS400；普康6066。
小鼠尿微量白蛋白ALB试剂盒使用方法
小鼠尿微量白蛋白（ALB）试剂盒使用方法检测范围：96T20μg/L -500μg/L使用目的：本试剂盒用于测定小鼠血清、血浆及相关液体样本中尿微量白蛋白（ALB）含量。
实验原理本试剂盒应用双抗体夹心法测定标本中小鼠尿微量白蛋白（ALB）水平。
用纯化的小鼠尿微量白蛋白（ALB）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入尿微量白蛋白（ALB），再与HRP标记的尿微量白蛋白（ALB）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的尿微量白蛋白（ALB）呈正相关。
用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中小鼠尿微量白蛋白（ALB）浓度。
试剂盒组成1 30倍浓缩洗涤液20ml×1瓶7 终止液6ml×1瓶2 酶标试剂6ml×1瓶8 标准品（960μg/L）0.5ml×1瓶3 酶标包被板12孔×8条9 标准品稀释液 1.5ml×1瓶4 样品稀释液6ml×1瓶10 说明书1份5 显色剂A液6ml×1瓶11 封板膜2张6 显色剂B液6ml×1/瓶12 密封袋1个标本要求1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。
若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融2．不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。
操作步骤1.标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。
480μg/L 5号标准品150μl的原倍标准品加入150μl标准品稀释液240μg/L 4号标准品150μl的5号标准品加入150μl标准品稀释液120μg/L 3号标准品150μl的4号标准品加入150μl标准品稀释液60μg/L 2号标准品150μl的3号标准品加入150μl标准品稀释液30μg/L 1号标准品150μl的2号标准品加入150μl标准品稀释液2.加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、标准孔、待测样品孔。
SOP标准操作程序-ALB
SOP标准操作程序ALB 白蛋白（货号：OSR6102，OSR6202）实验原理：血清白蛋白可用于众多疾病的诊断。
血清白蛋白升高通常见于脱水的。
血清白蛋白降低多见于肾脏疾病，肝脏疾病，感染性疾病，严重的烧伤和癌症。
1965年，Rodkey介绍了用一种中性缓冲液溴甲酚绿（BCG）作为指示剂直接测定血清白蛋白的浓度。
1971年，Doumas等人，对以上方法进行改良，加入了非离子表面活性剂，消除浊度，改善线性与敏感度。
奥林巴斯AU640的白蛋白是Doumas和Rodkey方法的改良方法，应用的是不同的缓冲系统。
在PH值4.0，溴甲酚绿与白蛋白形成强烈的绿色复合物。
白蛋白—溴甲酚绿的复合物在双波长（600/800nm）下的吸光度与白蛋白的浓度成正比。
PH 4.2白蛋白+溴甲酚绿---------------绿色化合物标本：病人准备：无特殊要求。
最好用禁食的标本以减少乳糜血的干扰。
类型：血清、肝素或EDTA血浆，标本最好不要溶血。
留取标本后请尽快分离血清/血浆。
标本稳定性：在室温条件下（15～25℃）可以稳定一周，在冰箱保存的条件下（2～8℃）稳定一个月。
仪器与材料:仪器：奥林巴斯AU640生化分析仪材料：奥林巴斯AU640白蛋白（ALB）参与反应成份的最终浓度：琥珀酸盐缓冲液（PH 4.2）100 mmol/L溴甲酚绿0.2 mmol/L其中含有稳定剂与保护剂。
注意：1.此试剂为体外诊断用。
2.不要入口，吞下有害。
试验用试管的直径在12～16mm。
定标液：奥林巴斯定标液（Cat. No. DR0040）试剂准备：奥林巴斯AU640的ALB试剂是即开即用的。
无需特殊准备。
执行参数（性能参数）：以下数据是根据奥林巴斯AU640 ALB试剂已经建立的程序。
重复性：重复性的评估是根据NCCLS推荐的典型方法，AU640批内重复性小于3%，总不精密度小于3%。
用于分析的质控血清和数据处理符合以上的NCCLS的规则。
小鼠尿微量白蛋白(ALB)说明书
小鼠尿微量白蛋白(ALB）酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定小鼠血清，血浆,相关液体样本尿微量白蛋白(ALB）含量。
实验原理：本试剂盒应用双抗体夹心法测定标本中小鼠尿微量蛋白(ALB)水平。
用纯化的小鼠尿微量蛋白(ALB)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入尿微量蛋白(ALB)，再与HRP标记的ALB抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的尿微量蛋白(ALB)呈正相关。
用酶标仪在450nm波长下测定吸光度（OD 值），通过标准曲线计算样品中小鼠尿微量蛋白(ALB)浓度。
试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×48 1×96 2-8℃保存标准品：540μg/L 0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液 1.5ml×1瓶 1.5ml×1瓶2-8℃保存酶标试剂 3 ml×1瓶 6 ml×1瓶2-8℃保存样品稀释液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂A液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液 3 ml×1瓶 6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如出现沉淀，应再次离心。
MouseUrinaryAlbuminDetectionKit小鼠尿白蛋白检测试剂盒说明书
MouseUrinaryAlbuminDetectionKit小鼠尿白蛋白检测试剂盒说明书Proteinurea is a common symptom of nephritis in humans and experimental animals, and is an important marker for evaluating disease severity regardless of the type of nephritis. Moreover, proteinurea is determined as the total amount of protein （serum proteins such as globulins and albumin） excreted into a 16hour urine collection, which is distinctly more accurate than determining the urinary protein concentration, due to large variations in urine volume between individual animals. The turbidity method is a commonly used urinary protein assay method, because it is accurate, easy, and economical.However, mouse kidneys leak serum components such as bilirubin, resulting in overestimated urinary protein levels, thus proteinuria is not a suitable marker for the evaluation of nephritis severity. On the other hand, albumin, a serum protein with a relatively small molecular weight, and typically the fi rst protein observed in the urine when kidney dysfunction begins to develop, is a more suitable marker to evaluate the severity of nephritis inmouse models.Chondrexs Mouse Urinary Albumin Detection Kit （catalog 3012） is designed to specifi cally determine mouse albumin in 40 urine samples within 4 hours using a sandwich immunoassay method. In mouse nephritis models, such as immune complexinduced glomerulonephritis （ICGN）, a mouse is deemed nephritic if the total albumin content in a16hour urine collection is greater than 1 mg. It is important to note that urinary albumin levels can reach up to 50200 mg in a 16hour urine collection, depending on the severity of nephritis.。
小鼠尿微量白蛋白(ALB)说明书.
小鼠尿微量白蛋白(ALB酶联免疫分析(ELISA试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定小鼠血清,血浆,相关液体样本尿微量白蛋白(ALB含量。
实验原理:本试剂盒应用双抗体夹心法测定标本中小鼠尿微量蛋白(ALB水平。
用纯化的小鼠尿微量蛋白(ALB抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入尿微量蛋白(ALB,再与HRP标记的ALB抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的尿微量蛋白(ALB呈正相关。
用酶标仪在450nm波长下测定吸光度(OD 值,通过标准曲线计算样品中小鼠尿微量蛋白(ALB浓度。
试剂盒组成:试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片(482片(96密封袋1个1个酶标包被板1×48 1×96 2-8℃保存标准品:540μg/L 0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液 1.5ml×1瓶1.5ml×1瓶2-8℃保存酶标试剂 3 ml×1瓶 6 ml×1瓶2-8℃保存样品稀释液 3 ml×1瓶6 ml×1瓶2-8℃保存显色剂A液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液 3 ml×1瓶 6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液(20ml×20倍×1瓶(20ml×30倍×1瓶2-8℃保存样本处理及要求:1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分。
仔细收集上清,保存过程中如出现沉淀,应再次离心。
2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分。
白蛋白(Alb)测定标准操作程序SOP文件
贮存条件：置2-8℃冰箱至有效期。
准备：直接使用。
质控间隔时间及限制：应视不同地区及各自实验室情况而定。质控结果应在限定的范围之内，如果超出范围，实验室应根据情况采取措施。
5仪器
ABCD医院
生化实验室
文件编号：
ABCD-SOP-04-11
白蛋白（Alb）测定
版序：ABCD
页码：第2页，共3页
低。在严重的低蛋白情况下，血浆白蛋白的浓度可以低达2.5g/dl。由于血浆的渗透压将低，水分通过毛细血管渗透进入组织。在白蛋白低于20g/l时，临床出现水肿症状。白蛋白的浓度监测可以提供进行饮食供给的提示和监测肝脏功能。
ALb参数设置表
TEST NAME [ ALB ]
R.VOLUME(R4) [ 0 ]
9注意事项
9.1应该在1小时内分离血浆或者血清。
9.2血清标本出现溶血、脂血、黄疸或抗坏血酸的干扰情况参见抗干扰能力。
9.3换算公式：g/dl×10 = g/l
9.4仅应用于体外诊断。
9.5扔弃废物应符合当地的法规。
10抗干扰能力：
10.1标准：回收率在90％－110％之间。
10.2黄疸：黄胆指数达到21时不会有明显干扰。（直接和间接胆红素浓度约为21mg/dl）。
高白蛋白没有很重要的临床意义，常见于脱水、休克等造成血液浓缩的情况下。
低白蛋白血症可以在很多种疾病和因素下发生：如肾脏疾病，肝脏疾病以及蛋白摄入过少，组织损伤以及蛋白丢失性肠道疾病均可以造成白蛋白水平的降
ABCD医院
生化实验室
文件编号：
ABCD-SOP-04-11
白蛋白（Alb）测定
版序：ABCD
页码：第3页，共3页
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小鼠尿微量白蛋白(ALB）酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定小鼠血清，血浆,相关液体样本尿微量白蛋白(ALB）含量。
实验原理：本试剂盒应用双抗体夹心法测定标本中小鼠尿微量蛋白(ALB)水平。
用纯化的小鼠尿微量蛋白(ALB)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入尿微量蛋白(ALB)，再与HRP标记的ALB抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的尿微量蛋白(ALB)呈正相关。
用酶标仪在450nm波长下测定吸光度（OD 值），通过标准曲线计算样品中小鼠尿微量蛋白(ALB)浓度。
试剂盒组成：样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如出现沉淀，应再次离心。
2. 血浆：应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如有沉淀形成，应该再次离心。
3. 尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如有沉淀形成，应再次离心。
胸腹水、脑脊液参照实行。
4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。
离心20分钟左右（2000-3000转/分）。
仔细收集上清。
检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。
通过反复冻融，以使细胞破坏并放出细胞内成份。
离心20分钟左右（2000-3000转/分）。
仔细收集上清。
保存过程中如有沉淀形成，应再次离心。
5. 组织标本：切割标本后，称取重量。
加入一定量的PBS，PH7.4。
用液氮迅速冷冻保存备用。
标本融化后仍然保持2-8℃的温度。
加入一定量的PBS（PH7.4），用手工或匀浆器将标本匀浆充分。
离心20分钟左右（2000-3000转/分）。
仔细收集上清。
分装后一份待检测，其余冷冻备用。
6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。
若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.7. 不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。
操作步骤1.标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在第一、第二孔中分别加标准品100μl，然后在第一、第二孔中加标准品稀释液50μl，混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。
（稀释后各孔加样量都为50μl，浓度分别为360μg/L，240μg/L ，120μg/L，60μg/L，30μg/L）。
2.加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。
在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5倍）。
加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
3.温育：用封板膜封板后置37℃温育30分钟。
4.配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用。
5.洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。
6.加酶：每孔加入酶标试剂50μl，空白孔除外。
7.温育：操作同3。
8.洗涤：操作同5。
9.显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15分钟.10.终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。
11.测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD值）。
测定应在加终止液后15分钟以内进行。
注意事项：1．试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。
2．浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。
3．各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。
一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。
4．请每次测定的同时做标准曲线，最好做复孔。
如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请最后乘以总稀释倍数（×n×5）。
5．封板膜只限一次性使用，以避免交叉污染。
6．底物请避光保存。
7．严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8．所有样品，洗涤液和各种废弃物都应按传染物处理。
9．本试剂不同批号组分不得混用。
10. 如与英文说明书有异，以英文说明书为准。
计算：在坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。
（此图仅供参考）试剂盒性能：1.样品线性回归与预期浓度相关系数R值为0.92以上。
2.批内与批见应分别小于9%和15%检测范围：20μg/L -400μg/L保存条件及有效期：1.试剂盒保存：；2-8℃。
2．有效期：6个月FOR RESEARCH USE ONLYDrug NamesGeneric Name ：Mouse microalbunminuria (ALB) ELISA Kit .PurposeThis kit allows for the determination of ALB concentrations in Mouse serum, blood plasma, tissue and other biological fluids.Principle of the assayT he kit assay Mouse ALB level in the sample ，use Purified Mouse ALB antibody to coatmicrotiter plate wells, make solid-phase antibody, then add ALB to wells, Combined ALB antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the colorchange is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ALB in the samples is then determined by comparing the O.D. of the samples to the standard curve.Materials provided with the kitSpecimen requirements1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.2.plasma-use suited EDTA or citrate or as an anticoagulant,mix 10-20 mins ,centrifugation20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m.remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.4.cell culture supernatant-detect secretory components, collect sue a sterile container,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidlyfrozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）,Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m.remove supernatant.6.extract as soon as possible after Specimen collection,and according to the relevantliterature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active. Assay procedure1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 360μg/L，240μg/L ，120μg/L，60μg/L，30μg/L)2.add sample：Set blank wells separately (blank comparison wells don’t ad d sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.7.incubate：Operation with 3.8.washing：Operation with 5.9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.Important notes1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes inthe room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.2.washing buffer will Crystallization separation, it can be heated the water helps dissolvewhen dilute . Washing does not affect the result.3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids theexperimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .4.if the testing material content is excessively higher (The sample OD is bigger than the firststandard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.6.The substrate evade the light preservation.7.Please according to use instruction strictly, The test result determination must take themicrotiter plate reader as a standard.8.All samples, washing buffer and each kind of reject should according to infective materialprocess.9.Do not mix reagents with those from other lots.CalculateAssay range20μg/L -400μg/LStorage and validity1．Storage ： 2-8℃. 2．validity ： six months.Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.This chart for reference only。