拟南芥atcwinv1基因T-DNA插入纯合突变体PCR鉴定及表型观察

拟南芥atcwinv1基因T-DNA插入纯合突变体PCR鉴定及

表型观察

阮燕晔;张莹;王波

【摘要】以拟南芥atcwinvl 基因T-DNA插入纯合突变体和野生型植株为材料,比较研究了2种基因型植株在营养期和生殖期的形态差异.结果表明:拟南芥atcwinvl 基因T-DNA插入纯合突变体(简称突变体)较野生型萌发率平均下降5.88个百分点;突变体在44 d抽薹,较野生型延后4d;分支数平均4支,较野生型下降20.84%;果荚开裂时间6d左右,较野生型延长2d;单株果荚数平均62.27个,较野生型降低

11.00%;单株果荚种粒数平均45.87粒,较野生型降低21.46%;突变体的单果荚长度平均14.52 cm,较野生型降低10.24%;单株果质量平均50.83mg,较野生型降低23.70%.拟南芥突变体在营养生长时期的株高平均10.44 cm,较野生型下降

21.03%;主根长平均7.62 cm,较野生型下降14.96%;单株莲座叶面积平均3.16 cm2,较野生型下降13.90%;单株地上部分鲜质量平均81.81mg,较野生型下降11.11%;单株根鲜质量平均6.21mg,较野生型下降17.64%;单株地上部分干质量平均6.17 mg,较野生型下降15.60%;单株根干质量平均0.55mg,较野生型下降

6.78%.拟南芥突变体在生殖生长时期的株高平均18.78 cm,较野生型增加4.22%;主根长平均16.48 cm,较野生型下降5.88%;单株莲座叶面积平均6.80 cm2,较野生型下降6.21%;单株地上部分鲜质量平均129.85 mg,较野生型下降9.69%;单株根鲜质量平均9.97 mg,较野生型下降13.23%;单株地上部分干质量平均9.22 mg,较野生型下降4.16%;单株根干质量平均0.70mg,较野生型下降6.67%.以上研究结果表明,atcwinvl 基因T-DNA插入突变影响了拟南芥植株正常的生长发育.%In the research, homozygous mutant (atcwinvl) and wild type of Arabidopsis were used to compare plant morphological differences in the period of

vegetative growth and reproductive growth. The results showed that compared to the wild type the germination rate of atcwinvl averagely decreased by 5.88 percentage points; compared to the wild type, bolting time of atcwinvl was 44 d, delayed 4 days;branch number of atcwinvl was 4, decreased by 20. 84 ％ ;cracking time of the pod of atcwinvl was 6,delayed 2 days;fruit pod number per plant of atcwinvl was 62.27,decreased by

11.00％ ,fruit number of a pod of atcwinvl was 45.87,decreased by

21.46％;length of a fruit pod of atcwinvl was 14.52cm,decreased by 10. 24％fruit weight of a plant of atcwinvl was 50. 83mg,decreased by 23.70％. In the period of vegetative growth,compared to the wild type, plant height of atcwinvl was 10.44cm, decreased by 21. 03％; root length of atcwinvl was 7.62cm, average decreased by 14. 96％; area of rosette leaf of atcwinvl was 3. 16 cm2 ,decreased by 13.90％ ;aboveground fresh weight of per plant was 81.81 mg, decreased by 11.11％ ;fresh weight of root per plant was

6.21mg, decreased by 1

7.64％ ;aboveground dry weight of per plant was 6.

17 mg, decreased by 15.60 ％ ;dry weight of root per plant was 0. 55

mg,decreased by 6. 78 ％ ;In the period of reproductive growth, compared to the wild type, plant height of atcwinvl was 18. 78cm,increased by

4.22％ ;root length of atcwinvl was 16.48cm,averagely decreased by

5.88％; area of rosette leaf of atcwinvl was

6.80 cm2, decreased by 6.21％; fresh weight of a plant of atcwinvl was 129.85 mg,decreased by 9.69％ ;fresh weight of root per plant was 9. 97 mg,decreased by 13.23％ ;aboveground dry weight of per plant was 9.22 mg, decreased by 4. 16％ ;dry weight of root per plant was 0. 70mg,decreased by 6.67％. The results obtained