孟德尔遗传学
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copyright cmassengale 17
Reproduction in Flowering Plants
Pollen contains sperm Produced by the stamen Ovary contains eggs Found inside the flower Pollen carries sperm to the eggs for fertilization Self-fertilization can occur in the same flower Cross-fertilization can occur between flowers
The theoretical or expected ratio of plants producing round or wrinkled seeds is 3 round :1 wrinkled Mendel’s observed ratio was 2.96:1 The discrepancy is due to statistical error The larger the sample the more nearly the results approximate to the theoretical ratio
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Genes and Environment Determine Characteristics
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15
Mendel’s Pea Plant Experiments
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16
Why peas, Pisum sativum?
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12
Genotype & Phenotype in Flowers
Genotype of alleles:
R = red flower r = yellow flower
All genes occur in pairs, so 2 alleles affect a characteristic Possible combinations are: Genotypes Phenotypes RR RED Rr RED
copyright cmassengale 25
Generation “Gap”
Parental P1 Generation = the parental generation in a breeding experiment. F1 generation = the first-generation offspring in a breeding experiment. (1st filial generation) From breeding individuals from the P1 generation F2 generation = the second-generation offspring in a breeding experiment. (2nd filial generation) From breeding individuals from the F1 generation
Can be grown in a small area Produce lots of offspring Produce pure plants when allowed to self-pollinate several generations Can be artificially cross-pollinated
copyright cmassengale
Monohybrid Crosses
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28
Trait: Seed Shape Alleles: R – Round r – Wrinkled Cross: Round seeds x Wrinkled seeds RR x rr
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Following the Generations
Cross 2 Pure Plants TT x tt
Results in all Hybrids Tt
Cross 2 Hybrids get 3 Tall & 1 Short TT, Tt, tt
27
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22
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23
Mendel’s Experimental Results
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24
Did the observed ratio match the theoretical ratio?
copyright cmassengale 11
More Terminology
Genotype - gene combination for a trait (e.g. RR, Rr, rr) Phenotype - the physical feature resulting from a genotype (e.g. red, white)
P1 Monohybrid Cross
r
r
Genotype: Rr
Phenotype: Round Genotypic Ratio: All alike Phenotypic Ratio: All alike
copyright cmassengale 29
R
R
百度文库
Rr
Rr
Rr
Rr
P1 Monohybrid Cross Review
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7
Types of Genetic Crosses
Monohybrid cross - cross involving a single trait e.g. flower color Dihybrid cross - cross involving two traits e.g. flower color & plant height
Eight Pea Plant Traits
Seed shape --- Round (R) or Wrinkled (r) Seed Color ---- Yellow (Y) or Green (y) Pod Shape --- Smooth (S) or wrinkled (s) Pod Color --- Green (G) or Yellow (g) Seed Coat Color ---Gray (G) or White (g) Flower position---Axial (A) or Terminal (a) Plant Height --- Tall (T) or Short (t) Flower color --- Purple (P) or white (p)
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How Mendel Began
Mendel produced pure strains by allowing the plants to selfpollinate for several generations
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Homozygous dominant x Homozygous recessive Offspring all Heterozygous (hybrids) Offspring called F1 generation Genotypic & Phenotypic ratio is ALL ALIKE
copyright cmassengale
6
Genetic Terminology
Trait - any characteristic that can be passed from parent to offspring Heredity - passing of traits from parent to offspring Genetics - study of heredity
copyright cmassengale 8
Punnett Square
Used to help solve genetics problems
copyright cmassengale
9
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10
Designer “Genes”
Alleles - two forms of a gene (dominant & recessive) Dominant - stronger of two genes expressed in the hybrid; represented by a capital letter (R) Recessive - gene that shows up less often in a cross; represented by a lowercase letter (r)
copyright cmassengale 3
Gregor Johann Mendel
Between 1856 and 1863, Mendel cultivated and tested some 28,000 pea plants He found that the plants' offspring retained traits of the parents Called the “Father of Genetics"
copyright cmassengale 4
Site of Gregor Mendel’s experimental garden in the Czech Republic
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5
Particulate Inheritance
Mendel stated that physical traits are inherited as “particles” Mendel did not know that the “particles” were actually Chromosomes & DNA
Austrian monk Studied the inheritance of traits in pea plants Developed the laws of inheritance Mendel's work was not recognized until the turn of the 20th century
Mendelelian Genetics
copyright cmassengale
1
Gregor Mendel
(1822-1884)
Responsible for the Laws governing Inheritance of Traits
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Gregor Johann Mendel
copyright cmassengale
rr YELLOW
13
Genotypes
Homozygous genotype - gene combination involving 2 dominant or 2 recessive genes (e.g. RR or rr); also called pure Heterozygous genotype - gene combination of one dominant & one recessive allele (e.g. Rr); also called hybrid
copyright cmassengale 18
Mendel’s Experimental Methods
Mendel hand-pollinated flowers using a paintbrush He could snip the stamens to prevent self-pollination Covered each flower with a cloth bag He traced traits through the several generations
Reproduction in Flowering Plants
Pollen contains sperm Produced by the stamen Ovary contains eggs Found inside the flower Pollen carries sperm to the eggs for fertilization Self-fertilization can occur in the same flower Cross-fertilization can occur between flowers
The theoretical or expected ratio of plants producing round or wrinkled seeds is 3 round :1 wrinkled Mendel’s observed ratio was 2.96:1 The discrepancy is due to statistical error The larger the sample the more nearly the results approximate to the theoretical ratio
copyright cmassengale 14
Genes and Environment Determine Characteristics
copyright cmassengale
15
Mendel’s Pea Plant Experiments
copyright cmassengale
16
Why peas, Pisum sativum?
copyright cmassengale
12
Genotype & Phenotype in Flowers
Genotype of alleles:
R = red flower r = yellow flower
All genes occur in pairs, so 2 alleles affect a characteristic Possible combinations are: Genotypes Phenotypes RR RED Rr RED
copyright cmassengale 25
Generation “Gap”
Parental P1 Generation = the parental generation in a breeding experiment. F1 generation = the first-generation offspring in a breeding experiment. (1st filial generation) From breeding individuals from the P1 generation F2 generation = the second-generation offspring in a breeding experiment. (2nd filial generation) From breeding individuals from the F1 generation
Can be grown in a small area Produce lots of offspring Produce pure plants when allowed to self-pollinate several generations Can be artificially cross-pollinated
copyright cmassengale
Monohybrid Crosses
copyright cmassengale
28
Trait: Seed Shape Alleles: R – Round r – Wrinkled Cross: Round seeds x Wrinkled seeds RR x rr
copyright cmassengale 26
Following the Generations
Cross 2 Pure Plants TT x tt
Results in all Hybrids Tt
Cross 2 Hybrids get 3 Tall & 1 Short TT, Tt, tt
27
copyright cmassengale 21
copyright cmassengale
22
copyright cmassengale
23
Mendel’s Experimental Results
copyright cmassengale
24
Did the observed ratio match the theoretical ratio?
copyright cmassengale 11
More Terminology
Genotype - gene combination for a trait (e.g. RR, Rr, rr) Phenotype - the physical feature resulting from a genotype (e.g. red, white)
P1 Monohybrid Cross
r
r
Genotype: Rr
Phenotype: Round Genotypic Ratio: All alike Phenotypic Ratio: All alike
copyright cmassengale 29
R
R
百度文库
Rr
Rr
Rr
Rr
P1 Monohybrid Cross Review
copyright cmassengale
7
Types of Genetic Crosses
Monohybrid cross - cross involving a single trait e.g. flower color Dihybrid cross - cross involving two traits e.g. flower color & plant height
Eight Pea Plant Traits
Seed shape --- Round (R) or Wrinkled (r) Seed Color ---- Yellow (Y) or Green (y) Pod Shape --- Smooth (S) or wrinkled (s) Pod Color --- Green (G) or Yellow (g) Seed Coat Color ---Gray (G) or White (g) Flower position---Axial (A) or Terminal (a) Plant Height --- Tall (T) or Short (t) Flower color --- Purple (P) or white (p)
copyright cmassengale 19
How Mendel Began
Mendel produced pure strains by allowing the plants to selfpollinate for several generations
copyright cmassengale 20
Homozygous dominant x Homozygous recessive Offspring all Heterozygous (hybrids) Offspring called F1 generation Genotypic & Phenotypic ratio is ALL ALIKE
copyright cmassengale
6
Genetic Terminology
Trait - any characteristic that can be passed from parent to offspring Heredity - passing of traits from parent to offspring Genetics - study of heredity
copyright cmassengale 8
Punnett Square
Used to help solve genetics problems
copyright cmassengale
9
copyright cmassengale
10
Designer “Genes”
Alleles - two forms of a gene (dominant & recessive) Dominant - stronger of two genes expressed in the hybrid; represented by a capital letter (R) Recessive - gene that shows up less often in a cross; represented by a lowercase letter (r)
copyright cmassengale 3
Gregor Johann Mendel
Between 1856 and 1863, Mendel cultivated and tested some 28,000 pea plants He found that the plants' offspring retained traits of the parents Called the “Father of Genetics"
copyright cmassengale 4
Site of Gregor Mendel’s experimental garden in the Czech Republic
copyright cmassengale
5
Particulate Inheritance
Mendel stated that physical traits are inherited as “particles” Mendel did not know that the “particles” were actually Chromosomes & DNA
Austrian monk Studied the inheritance of traits in pea plants Developed the laws of inheritance Mendel's work was not recognized until the turn of the 20th century
Mendelelian Genetics
copyright cmassengale
1
Gregor Mendel
(1822-1884)
Responsible for the Laws governing Inheritance of Traits
copyright cmassengale 2
Gregor Johann Mendel
copyright cmassengale
rr YELLOW
13
Genotypes
Homozygous genotype - gene combination involving 2 dominant or 2 recessive genes (e.g. RR or rr); also called pure Heterozygous genotype - gene combination of one dominant & one recessive allele (e.g. Rr); also called hybrid
copyright cmassengale 18
Mendel’s Experimental Methods
Mendel hand-pollinated flowers using a paintbrush He could snip the stamens to prevent self-pollination Covered each flower with a cloth bag He traced traits through the several generations