5-氨基酮戊酸光动力疗法治疗痤疮过程中炎症因子的动态检测

摘要：目的 利用兔耳痤疮模型探讨 5-氨基酮戊酸光动力疗法（ALA-PDT）治疗痤疮过程中不同时间点炎症因子表达水平的动
态变化。方法 皮内注射痤疮丙酸杆菌（C.acnes）于兔耳皮肤，构建兔耳痤疮模型。成功构建的模型兔耳给予 ALA-PDT 治疗。通
过组织 HE 染色评价痤疮炎症的变化；提取兔耳组织 RNA 并以 Real-time PCR 检测 ALA-PDT 治疗前及治疗后 4、24 h 及 7 d
Y ANG Chen 1, 2, ZHA NG Ling-lin 2, LIU Xiao-jing 2, ZOU Ting 2, WANG Xiu-li 1, 2 * (1. Yangzhou University Medical College, Yangzhou 225000; 2. Optical Medicine Institute of Tongji University in Shanghai
Skin Disease Hospital, Shanghai 200443, China)
ABSTRACT: Objective To explore the dynamic changes of inflammatory factors expression at different time points during the treatment of acne with 5 -aminolevulinic acid photodynamic therapy (ALA -PDT) based on rabbit ear acne model. Methods A rabbit ear acne model was established by intradermal injection of Cutibacterium acnes (C.acnes) into the skin of rabbit ears. The successfully established acne lesions were treated with ALA-PDT. HE staining was used to evaluate the variation trend of tissue inflammatory response. RNA was extracted from rabbit ear tissue, and real-time PCR was used to detect the relative expression levels of inflammatory factors such as IL-1琢, IL-1茁, IL-6, IL-8 and NF-资B (p65) mRNA before and at 4, 24 h and 7 days after ALA-PDT treatment. Results By evaluating the HE staining, it was found that the inflammation performance of skin lesion aggravated gradually at 4-24 hours after ALA-PDT treatment, and the infiltration of inflammatory cells decreased significantly at 7 days after ALA-PDT treatment. Real-time PCR showed that the relative expression levels of IL-1琢，IL-1茁 and IL-6 mRNA increased at 4 and 24 hours after ALA-PDT treatment compared with those before treatment, but the differences were not statistically significant (P>0.05), while the relative expression levels of IL -8 and p65 mRNA increased significantly, and the differences were statistically significant (P <0.05); the relative expression levels of all inflammatory factors decreased significantly at 7 days after ALA -PDT treatment compared with those before treatment, and the differences were statistically significant (P<0.05). Conclusion ALA -PDT may amplify inflammation by up -regulating IL -8 and p65 in the early stage, then inhibits inflammatory factors to improve tissue inflammation, and finally effectively treat acne. KEYWORDS: 5 -aminolevulinic acid; photodynamic therapy; Cutibacterium acnes; rabbit ear acne model; inflammatory factor
的炎症因子 IL-1琢、IL-1茁、IL-6、IL-8、NF-资B（p65）mRNA 的相对表达水平。结果 通过评估组织 HE 结果发现，ALA-PDT 治疗
后 4耀24 h 皮损区炎症逐渐加重，治疗后 7 d 组织炎症细胞浸润明显减轻。Real-time PCR 发现，与治疗前相比，ALA-PDT 治
最终有效治疗痤疮。
关键词：5-氨基酮戊酸；光动力疗法；痤疮丙酸杆菌；兔耳痤疮模型；炎症因子
中图分类号：R758.733
文献标志码：A
文章编号：2096-1413(2019)13-0001-04
Dynamic detection of inflammatory faLeabharlann Baidutors during 5-aminolevulinic acid photodynamic therapy for acne
2019 年 5 月
研究原著
5-氨基酮戊酸光动力疗法治疗痤疮过程中炎症因子的 动态检测
杨 晨 1，2，张 玲 琳 2，柳 小 婧 2，邹 婷 2，王 秀 丽 1，2* （1 . 扬 州 大 学 医 学 院 ，江 苏 扬 州 ，225000 ；2 . 上 海 市 皮 肤 病 医 院 同 济 大 学 医 学 院 光 医 学 研 究 所 ，上 海 ，200443）
疗后 4、24 h 的 IL-1琢、IL-1茁 和 IL-6 mRNA 的相对表达水平上升，但差异无统计学意义（P>0.05），而 IL-8、p65 mRNA 相
对表达水平明显升高，差异具有统计学意义（P约0.05）；治疗后 7 d，所有炎症因子相对表达水平均较治疗前显著下降，差异均具
有统计学意义（P约0.05）。结论 ALA-PDT 早期可能通过上调 IL-8 和 p65 放大炎症反应，随后抑制炎症因子改善组织炎症反应，