双语分子生物学复习资料

A Ori A
B Ori B
C Ori C （ 细菌的起始序来自百度文库 ） D Ori R
改下题目：在真核生物里面酵母菌的复制起始点
Ori C 和 Ori R 又叫什么
ARS
1． RF
replicative form
复制叉
2． In lactose operon, the lacZ gene expression is induced by rather than （而不是） lactose
D)
C the intervening sequence between the –35 and –10 sequences is conserved.（ 保守的 ） .
D the distance between the –35 and –10 sequences is critical for transcription efficiency. 3. E.coli release factor1 (RF1 ) recognizes which codons? ( D )
立身以立学为先，立学以读书为本
1. （核酸性质 — 减色性， 双链 DNA ，单链 DNA, 寡核苷酸 ）Which has the highest absorption
per unit mass at a wavelength of 260 nm?
(
B)
A RNA. B mononucleotides.
菌） DNA replication except ( A ).
ADnaA BDnaBCDnaG D RPA
BCD 在起始的时候发挥作用
15.The yeast（酵母 ） replication origin is called autonomously replicating sequence (自主复 制序列 ARS ), also called ( D ).
B Telomere is long stretches at the ends of the chromosome and rich in G-C.
C Telomerase is the reverse transcriptase responsible for the extension of telomeric repeat
A PCR uses thermostable（ 热稳定的 ） DNA polymerases. B ideally, PCR primers should be of similar length and G+C content. C PCR optimization usually includes varying the magnesium（镁离子 ） concentration and the
C hydrophobic interaction （疏水相互作用） D peptide bonds （肽键） 核酸是 3，5 磷酸
二酯键
21. Which one of the following statements about DNA secondary structure is not
true? ( D
2. Which one of the following statements about transcription in E.coliis true? ( A the–10 sequence is always exactly 10 bp upstream from the transcription start site. B the initiating nucleotide is always a G .
The sequence 5’-AGTCTGACT- 3’ in DNA is equivalent to which sequence in
RNA?( C )—— 给你 DNA 序列，找到对应的 RNA 序列，给你 RNA 序列，找到对应 的 DNA 序列。
A5’-AGUCUGACU- 3’B5’-UGTCTGUCT- 3’
(B
), respectively.
ADNA pol a and DNA pol B δDNApol δ and DNA pol a CDNA pol εand DNA pol δDDNA pol a and DNA pol β
δ70 因子非常重要
10.In eukaryotes, mtDNA( 线粒体 DNA) is replicated by the ( C ).
polymerization temperature（聚合温度） .镁离子浓度非常重要，不能随意调整浓度，一 般恒定。 D if PCR was 100% efficient ,one target molecule would amplify to 2 n after n cycles.
2（. RNA 加工，结合时）Which one of the following statements correctly describes the splicing process undergone by most eukaryotic pre-mRNAs? ( C ) A in the first step of splicing the G at the 3 -end of the intron is’joined to the 2 -hydroxyl （ 羟’基）
A Telomere contains up to hundreds copies of a short repeated sequence.
BTeromerase is a typical DNA polymerase. （ 端粒酶不是经典的 DNA 聚合酶 ）
C The activities of telomerase are related to the development stages and tissue types. D The telomere is synthesized by the enzyme telomerase and DNA polymerase.
ADNA pol a B DNA pol δC DNA pol γ （DNA聚合酶 γ ） D DNA pol β
14. The following replication factors（复制因子） bind DNA in the initiation of bacterial （细
立身以立学为先，立学以读书为本
)
A The basic features of DNA double helix were deduced by James D. Watson and Francis Crick in 1953l. B The structure identified by James D. Watson and Francis Crick is known as B-form DNA which is right-handed form （右手螺旋） . C Under conditions of low humidity, DNA can be induced to form A-form DNA . D Z-form DNA is right -handed like the B-form DNA . （ 是左手 ）
D Telomerase is complex of DNA, protein and RNA.
8 种必须氨基酸 14.Which is the major forces between the amino acids in the polypeptides (多
肽链 )? (
)
A H-bonds and disulfide bonds （二硫键） B base stacking interaction （碱基堆积力）
C double-stranded DNA.
D protein.
减色性跟紫外最大吸收峰 Nucleotides （ 260）〉 RNA 〉 ssDNA>double-stranded DNA
4.（ RNA 的转录时， DNA 的模板链和编码链（有义链和反义链） ） mRNA 互补（）的链是
模板链（或称反义链，与反义链互补的是有义链） 。
立身以立学为先，立学以读书为本
For example: telomere replication 端粒复制
1． bpbase pairs碱基对 2． GMO Genetically modified organism 基因修饰生物
3．The approximate purity of dsDNA preparations may be estimated by determination of the
C 5’-UCAGUCUGA- 3’D 5’-AGUCAGACU- 3’
DNA 双链维持的结构是什么？
A H-bonds and disulfide bonds （二硫键） B base stacking interaction（碱基堆积力） and peptide bonds
C hydrophobic interaction （疏水相互作用） and H-bonds
11. Which one of the following statements about telom（er端e 粒） and telomerase
（端粒酶） is true? ( C )（概念）
A The activity of telomerase is very low in cancer and germ cell.
6.The operon model consists of 启动子、操纵子、 structrure gene and regulation genes（调
节基因） .
1. Which one of the following statements about PCR is false? ( C )
ratio of absorbance at 260 and 280 nm ( A260/A280), pure dsDNA has an A260/A280 of 1.8, and
pure RNA one of around 2.0.
4. mononucleotide 单核苷酸 5. 核酸 Nucleic acid 6. 染色体 chromosome 7.Gly and pro(脯氨酸 ) often occur in the beta-turn of protein secondary structure 8. 核苷酸 Nucleotide, ，核苷 Nucleoside ，核小体 Nucleosome
D base stacking interaction and hydrophobic interaction 碱基堆积和疏水相互作用
10.Which one of following statements about telomere（端粒） and teromerase（端粒酶） is
false?( B )
RF2 识别 UGA and UAA
A UAA only. B UAG only.
C UGA and UAA. DUAG and UAA.
5. Which topoisomerase is to segregate daughter DNA after replication in prokaryote?
（D
） 原核生物 DNA 复制好后怎么把酯链的 DNA 分开？
A topoisomerase (拓扑异构酶 ) I C topoisomerase III
B topoisomerase II D topoisomerase IV
8.In eukaryotes, the leading and lagging strands are synthesized by DNA polymerase
itself, however, in our experiment, is used to add to themedium （培养基） for induction of
lacZ gene expression.异乳糖比乳糖诱导作用更强
3． primer 引物
4. replication 复制
5. tanscription 转录