通用引物序列

PCMV-R
5'-ATT ATA GAG GAC ACC TAG TC-3'
PDONR-F
5'-TCG CGT TAA CGC TAG CAT GGA TCT C-3'
PDONR-R
5'-GTA ACA TCA GAG ATT TTG AGA CAC-3’
PEF-F
5'-TCA AGC CTC AGA CAG TGG TTC-3'
pLEXA-R
TAA AAC CTA AGA GTC ACT TT
pLNCX-F
AGC TCG TTT AGT GAA CCG TCA GAT CG
pLNCX-R
ACC TAC AGG TGG GGT CTT TCA TTC CC
pDSRED-N-R
TGA AGC GCA TGA ACT CCT TG
PCDNA3.0-R2
5'-GGC AAC TAG AAG GCA CAG TC-3'
PCDNA3.1F
5'-CTA GAG AAC CCA CTG CTT AC-3’
PCDNA3.1R
5'-TAG AAG GCA CAG TCG AGG-3’
pCEP Frorward
5'-AGC AGA GCT CGT TTA GTG AAC CG-3'
PCEP-F
5'-AGA GCT CGT TTA GTG AAC CG-3'
PCMV-F
5'-TCT AAA AGC TGC GGA ATT GT-3'
PCMV-F
5'-TTC CAA AAT GTC GTA ATA AC-3'
PCMV-R
5'-TCC AAA CTC ATC AAT GTA TC-3'
PEGFP-C-3’
5'-TAT GGC TGA TTA TGA TCA GT-3'
PEGFP-C-5’
5'-CAT GGT CCT GCT GGA GTT CGT G-3'
PEGFP-N-3’
5'-CGT CGC CGT CCA GCT CGA CCA G-3'
PEGFP-N-5’
5'-TGG GAG GTC TAT ATA AGC AGA G-3'
S.TAG
5'-CGA ACG CCA GCA CAT GGA CA-3'
S1
5'-CAA CGT GAA AAA ATT ATT ATT CGC-3’
S6
5'-GTA AAT GAA TTT TCT GTA TGA GG-3’
SP6
5'-ATT TAG GTG ACA CTA TAG-3'
T3
5'-ATT AAC CCT CAC TAA AGG GA-3'
PIRES3-EGFP-R
5'-AAC GCA CAC CGG CCT TAT TC-3'
pMAL-C2X-F
5'-TGC GTA CTG CGG TGA TCA AC-3’
pMAL-C2X-R
5'-CTG CAA GGC GAT TAA GTT GG-3’
PQE30F
5'-TGA GCG GAT AAC AAT TTC AC-3'
TRXR
5'-GGA TCC CTT GTC ATC GTC ATC ACC A-3'
malEfor
5'-GGT CGT CAG ACT GTC GAT GAA GCC-3'
pTrcHis-fw
5'-TTA AAG AGG TAT ATA TTA ATG TAT CG-3'
Xpressfor
5'-AGC ATG ACT GGT GGA CAG-3'
PSG5-R(SV40)
5'-AAA CCA CAA CTA GAA TGC AGT-3'
M13R(-26)
5'-CAG GAA ACA GCT ATG AC-3'
pBRforBam
5'-CTT GGA GCC ACT ATC GAC-3'
pBRrevBam
5'-GGT GAT GTC GGC GAT ATA GG-3'
pBRforEco
5'-AAT AGG CGT ATC ACG AGG C-3'
pBRrevHind
5'-GCG TTA GCA ATT TAA CTG TG-3'
c-mycrev
5'-CTC TTC TGA GAT GAG TTT TTG-3'
pGAL4-ADrv
5'-GAA CTT GCG GGG TTT TTC-3'
T7
5'-TAA TAC GAC TCA CTA TAG GG-3'
T7（17BASE）
5'-ACA TCC ACT TTG CCT TTC TC-3'
T7TER
5'-TGC TAG TTA TTG CTC AGC GG-3'
TRXF
5'-CAT ATG AGC GAT AAA ATT ATT CAC-3'
M13F（-47）
5'-CGC CAG GGT TTTC CCA GTC ACG AC-3'
M13R
5'-CAG GAA ACA GCT ATG ACC-3'
M13R（-48）
5'-AGC GGA TAA CAA TTT CAC ACA GGA-3'
P24990
5'-TCATCGGAAGCGAGTAG-3'
pETup
5'-ACG ATG CGT CCG GCG TAG AGG A-3'
PGEX-3’
5'-CCG GGA GCT GCA TGT GTC AGA GG-3'
PGEX-5’
5'-GGG CTG GCA AGC CAC GTT TGG TG-3'
PIRES2-EGFP-F
5'-GTA GGC GTG TAC GGT GGG AG-3'
PTRCHISA-F
5'-CAT GGT ATG GCT AGC ATG AC-3’
PTRCHISA-R
5'-CAG GCT GAA AAT CTT CTC TC-3’
RVP3
5'-CTA GCA AAA TAG GCT GTC CC-3'
RVP4
5'-GAC GAT AGT CAT GCC CCG CG-3'
A-FACTOR
5'-TAC TAT TGC CAG CAT TGC TGC-3'
BAC1
5'-AAC CAT CTC GCA AAT AAA TA-3'
BAC2
5'-ACG CAC AGA ATC TAG CGC TT-3'
BGH rev
5'-TAG AAG GCA CAG TCG AGG C-3'
5'-GAA TAA GTG CGA CAT CAT CAT C-3’
pBiblioteka BaiduBLeu-3'
5'-CAG ATG GAA CGG TCT TTA AAT G-3’
HGHrev
5'-CTG GGG AGG GGT CAC AGG G-3'
PSG5(BAMHI)-F
5'-GCT GGT TAT TGT GCT GTC TC-3'
EBV Reverse
5'-TGT GGT TTG TCC AAA CTC AAT C-3'
EBV-R
5'-GTG GTT TGT CCA AAC TCA TC-3'
GAL4 AD-F
5'-TAC CAC TAC AAT GGA TG-3'
GAL4 BD
5'-TCA TCG GAA GAG AGT AG-3'
5'-CTC GGG AAG CGC GCC ATT GTG TTG G-3'
Primer TriplEx-LDrv-3'
5'-ATA CGA CTC ACT ATA GGG CGA ATT GGC-3'
UB-F
5'-TCA GTG TTA GAC TAG TAA ATT-3'
27F
5'-AGA GTT TGA TCC TGG CTC AG-3’
引物名称
引物序列
3’AOX
5'-GGC AAA TGG CAT TC TGA CAT-3'
3'AD
5'-AGA TGG TGC ACG ATG CAC AG-3'
3'BD
5'-TAA GAG TCA CTT TAA AAT TTG TAT C-3'
5’AOX1
5'-GAC TGG TTC CAA TTG ACA AGC-3'
pTrcHis-rv
5'-ATC TGT ATC AGG CTG AAA ATC-3'
N-CMV-30
5'-ATA ACC CCG CCC CGT TG-3'
C-CMV-24
5'-TTA GGA CAA GGC TGG TGG-3'
CMV-Profor
5'-ATG GGC GGT AGG CGT G-3'
1492R
5'-GGY TAC CTT GTT ACG ACT T-3’
PTR5
5'-CTC CGA GAT CTG GAC GAG C-3'
pLXSN-F
CTT GAA CCT CCT CGT TCG AC
pLXSN-R
GTT GCT GAC TAA TTG AGA TG
pLEXA-F
CGT CAG CAG AGC TTC ACC AT
CMV-F
5'-CGC AAA TGG GCG GTA GGC GTG-3'
CMV-R
5'-GTT CAC GGT GCC CTC C-3'
DsRed1-C-r
5'-AGC TGG ACA TCA CCT CCC ACA ACG-3'
DsRed1-N-f
5'-GTA CTG GAA CTG GGG GGA CAG-3'
PB42ADF
5'-CCA GCC TCT TGC TGA GTG GAG ATG-3'
PB42ADR
5'-AAG CCG ACA ACC TTG ATT GGA G-3'
pbd-gal4-f
5'-GCC TCT AAC ATT GAG ACA GC-3'
pbd-gal4-r
5'-AAG AGT TAC TCA AGA ACA AGA A-3'
P31430
5'-CGT TTT AAAACCTAAGAGTCAC-3'
P12584
5'-TTT TCA GTA TCT ACG AT-3’
P17110
5'-TAC CAC TAC AAT GGA TG-3’
PAS2-1F
5'-TCA TCG GAA GCG AGT AG-3'
PAS2-1R
5'-CGT TTT AAA ACC TAA GAG TCA C-3'
pCAMBIA1301-5'(ECORI)
5'-CCA GGC TTT ACA CTT TAT GC-3'
pCAMBIA1301-3'(HindIII)
5'-GCG ATT AAG TTG GGT AAC GC-3'
T7 EEV
5'-ATG TCG TAA TAA CCC CGC CCC G-3'
pDBLeu-5'
mtDNA-D-LoopF
AGG ACT Acg gct tga aaa gc
mtDNA-D-LoopR
atg tgc ctg acc gag gaa cca g
H1primer
CGCTATGTGTTCTGGGAAAT
pRECEIVER-M03F
5'-GCGGTAGGCGTGTACGGT-3'
pRECEIVER-M03R
pGAL4-BDrv
5'-AAT CAT AAG AAA TTC GCC C-3'
pMD19T490R
5'-CACAGGAAACAGCTATGACC-3'
Psos5'
5'-CCA AGA CCA GGT ACC ATG-3'
Psos3'
5'-GCC AGG GTT TTC CCA GT-3'
Primer TriplEx-LD-5´
5'-CCGGACACGCTGAACTTGT-3'
ACYCDuetUP1Primer #71178-3
GGATCTCGACGCTCTCCCT
DuetUP2 Primer
TTGTACACGGCCGCATAATC
pbi-121(35s)
5'-GAC GCA CAA TCC CAC TAT CC-3'
PBV220F
5'-AAG AAG GGC AGC ATT CAA AG-3'
PBV220R
5'-CTG CGT TCT GAT TTA ATC TG-3'
PCDNA3.0-F2
5'-CTC TGG CTA ACT AGA GAA CC-3'
GLP1
5'-TGT ATC TTA TGG TAC TGT AAC TG-3'
GLP2
5'-CTT TAT GTT TTT GGC GTC TTC CA-3'
M13(-96)
5'-CCC TCA TAG TTA GCG TAA CG-3'
M13F
5'-TGT AAA ACG ACG GCC AGT-3'
PQE30R
5'-GTT CTG AGG TCA TTA CTG G-3'
pTARGET-SEQ
5'-TTA CGC CAA GTT ATT TAG GTG ACA-3’
PTRC99C-F
5'-TTG CGC CGA CAT CAT AAC-3’
PTRC99C-R
5'-CTG CGT TCT GAT TTA ATC TG-3’