A Functional Approach to Generation with TAG 1
Activation Tagging Identifies a Conserved MYB Regulator of

The Plant Cell, Vol. 12, 2383–2393, December 2000, © 2000 American Society of Plant Physiologists Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid BiosynthesisJustin O. Borevitz,a,1 Yiji Xia,a,b,1 Jack Blount,b Richard A. Dixon,b and Chris Lamb a,2a Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037b Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401Plants produce a wide array of natural products, many of which are likely to be useful bioactive structures. Unfortu-nately, these complex natu ral produ cts u su ally occu r at very low abu ndance and with restricted tissu e distribu tion,thereby hindering their evaluation. Here, we report a novel approach for enhancing the accumulation of natural prod-ucts based on activation tagging by Agrobacterium-mediated transformation with a T-DNA that carries cauliflower mo-saic virus 35S enhancer sequences at its right border. Among ف5000 Arabidopsis activation-tagged lines, we found a plant that exhibited intense purple pigmentation in many vegetative organs throughout development. This upregulationof pigmentation reflected a dominant mu tation that resu lted in massive activation of phenylpropanoid biosyntheticgenes and enhanced accumulation of lignin, hydroxycinnamic acid esters, and flavonoids, including various anthocya-nins that were responsible for the purple color. These phenotypes, caused by insertion of the viral enhancer sequencesadjacent to an MYB transcription factor gene, indicate that activation tagging can overcome the stringent genetic con-trols regulating the accumulation of specific natural products during plant development. Our findings suggest a func-tional genomics approach to the biotechnological evaluation of phytochemical biodiversity through the generation ofmassively enriched tissue sources for drug screening and for isolating underlying regulatory and biosynthetic genes. INTRODUCTIONEthnobotany and limited screens of medicinal plants indi-cate that the huge repertoire of chemical diversity in plants contains many potentially useful bioactive structural princi-ples for developing novel drug s, flavors, frag rances, and other specialty chemicals. Unfortunately, these complex natural products usually occur in very low abundance and with a restricted tissue distribution. Moreover, almost all of this phytochemical biodiversity resides in exotic, unculti-vated species. Whereas drug s such as taxol, vinblastine, and vincristine illustrate the potential of plants as sources of new drugs, the development of rational approaches for the g eneration of useful amounts of complex natural products for industrial evaluation remains an unsolved problem. In particular, an intense 30-year effort using cell and tissue cul-tures from medicinal plants has failed to g enerate useful quantities of complex products for the commercial produc-tion of established drugs in vitro or for high-throughput, mul-tiplex screening of phytochemicals (Facchini and Deluca, 1995; McCaskill and Croteau, 1998). This failure probably reflects the string ent spatial and temporal transcriptional controls governing the biosynthesis of specific natural prod-ucts during plant development (Fowler, 1983; Robins, 1994; Facchini and Deluca, 1995). Transg enic manipulation to override these genetic controls thus may provide the key to enhancing natural product biosynthesis for industrial evalu-ation and exploitation.Activation tagging with the enhancer from the cauliflower mosaic virus 35S transcript promoter (35Se) is an emerging technology in plant functional genomics (Weigel et al., 2000). This approach, based on Agrobacterium-mediated transfor-mation, can create transgenic plants in which the T-DNA car-rying 35Se at its right border is spliced into the plant genome at random sites. In each independent transgenic line, 35Se strongly activates the plant gene to which, by chance, it lies adjacent. Activation of a gene in this fashion may lead to ob-servable effects on the modified plant, providing important clues about the function of the activated g ene. Screening larg e collections of independent, activation-tag g ed lines thus represents a powerful way of surveying the g enome and isolating genes that affect traits of interest.Using activation tagging, we have isolated a bright-purple mutant, pro ductio n o f antho cyanin pigment 1-Dominant (pap1-D), in which genes encoding enzymes involved in the biosynthesis of phenylpropanoid natural products exhibit massive and widespread activation throughout plant de-velopment. The pap1-D phenotype, which is caused by1These authors contributed equally to this work.2To whom correspondence should be addressed. E-mail mb @; fax 44-1603-456844.2384The Plant CellFigure 1.pap1-D Phenotypes.(A)pap1-D (left) and Col-0 (right) flowers.(B) Roots of pap1-D (left) and Col-0 (right) plants.(C) Six-week-old adult pap1-D (front) and Col-0 (back) plants.Activation Tagging of PAP12385 overexpression of a g ene encoding an MYB transcriptionfactor, indicates that activation tagging can be used to over-come the stringent genetic controls regulating the develop-mental accumulation of specific natural products. Thesefindings suggest a new approach for the systematic biotech-nological evaluation of phytochemical biodiversity through theg eneration of massively enriched tissue sources for drugscreening and for isolation of the underlying regulatory andbiosynthetic genes.RESULTSMutant CharacterizationApproximately 5000 activation-tagged primary lines of Ara-bidopsis ecotype Columbia (Col-0) were generated by usingpSKI015, which contains four copies of 35Se at the rig htborder of the T-DNA, pBluescript KSϩ for plasmid rescue,and the BAR g ene for Basta resistance as a selectablemarker (Kardailsky et al., 1999; Weigel et al., 2000). A singlebright-purple plant was observed in this collection, and itsseed was collected for prog eny analysis. T2 plants seg re-g ated for the purple coloration characteristic of anthocya-nins in a 3:1 ratio, which is consistent with this trait being determined by a single dominant allele, an allele we named pap1-D (see above). The whole plant, including the roots, stems, leaves, primary and secondary branches, and cauline leaves as well as sepals, anthers, and carpels, ex-hibited purple pigmentation (Figures 1B and 1C). The purple coloration was more pronounced when plants were grown under high-intensity light or other stress conditions, such as droug ht and pathog en infection (data not shown). Under these conditions, leaves and stems of wild-type plants also show a slig ht pig mentation, sug g esting that the pap1-D phenotype might in part reflect enhancement of an endoge-nous stress response. However, we never observed pig-mentation in the roots of wild-type plants—in marked contrast to the strong pigmentation at the base of pap1-D roots (Fig ure 1B). Except for very weak pig mentation in flower petals (Figure 1A), enhanced pigmentation in pap1-D was observed throughout development. No other morpho-logical phenotypes were observed.Because anthocyanins are a subclass of flavonoid natural products derived from the phenylpropanoid skeleton, we examined the expression of phenylpropanoid biosynthetic g enes and the accumulation of natural products. RNA g el blot analysis showed massive enhancement of the expres-sion of phenylpropanoid biosynthetic g enes in pap1-D plants (Figure 2). The amounts of transcripts encoding chal-cone synthase (CHS), the entry point enzyme into the fla-vonoid branch pathway, and dihydroflavonol reductase, an enzyme of flavonoid biosynthesis specific for anthocyanins, were greater in pap1-D plants than in wild-type Col-0 plants.Transcripts encoding g lutathione S-transferase, which has been implicated in the transport of anthocyanins into the vacuole (Alfenito et al., 1998), also were expressed in in-creased amounts. Moreover, the accumulation of tran-scripts that encode phenylalanine ammonia-lyase (PAL), the first enzyme of the overall phenylpropanoid biosynthetic pathway, also was markedly enhanced, indicating that tran-scriptional activation was not confined to the flavonoid branch.To determine the extent of changes in anthocyanins and other phenylpropanoid-derived compounds in pap1-D, we extracted and analyzed soluble and cell wall–bound phe-nolic compounds by HPLC. Analysis of the soluble fraction, which was obtained by extraction in acetone, revealed sev-eral quantitative differences between pap1-D and wild-type Col-0 plants—in particular, increased concentrations of cer-tain flavonol g lycosides, including Glc-rhamnose (Rha)-quercetin, Glc-Rha-kaempferol, and unidentified conjugates of kaempferol and quercetin (Figures 3A and 3B). After alka-line hydrolysis of the residue that was obtained after ace-tone extraction, one portion was freeze-dried for analysis of anthocyanidins (anthocyanin aglycones); the remainder was partitioned into ethyl acetate for determination of cell wall–bound hydroxycinnamic acids. Anthocyanidins were present in g reater concentrations in pap1-D than in Col-0 (Fig ures 3C and 3D), as were coumaric and sinapic acids measured in alkaline hydrolysates of the wall-bound phenolic fraction (Fig ures 3E and 3F). Thus, pap1-D is characterized by strongly increased concentrations of glycosylated anthocya-nins, flavonols, and cell wall–esterified hydroxycinnamic acidsin comparison with wild-type Col-0.Figu re 2.Enhanced Expression of Phenylpropanoid Biosynthetic Genes in pap1-D.RNA g el blot hybridization was conducted with total RNA isolated from 6-week-old pap1-D and Col-0 wild-type plants. DFR, dihydrofla-vonol reductase; GST, glutathione S-transferase; UBQ, ubiquitin.2386The Plant CellThe observation of increased wall-bound hydroxycin-namic acids in pap1-D prompted us to measure the content and composition of lignin, which is derived from hydroxycin-namic acid precursors. Lig nin was analyzed by derivati-zation followed by reductive cleavag e, which helps to determine the absolute amounts of guaiacyl (G, monomethyl-ated) and syringyl (S, dimethylated) lignin monomers (Lu and Ralph, 1997). The results in Table 1 indicate increases in both total G and total S residues in the cell wall fraction of pap1-D compared with those in Col-0, but the S/G ratio var-ied little. The change in lignin monomers could reflect an in-crease in lignin content or a change in composition that led to more efficient monomer extractability.Changes in lignin content and composition have been en-g ineered in transg enic plants by downreg ulation of PAL,caffeic acid O -methyltransferase, and caffeoyl-CoA O -meth-yltransferase, enzymes of the lig nin branch of phenylpro-panoid biosynthesis (Atanassova et al., 1995; Zhong et al.,1998). PAL activity in stems of pap1-D plants was approxi-mately twice that found in stems of wild-type plants,whereas the activities of the two O -methyltransferases dif-fered little between the two (Table 1). Thus, the changes in lig nin composition and increased concentrations of wall-bound sinapic acid in pap1-D reflect the change in PAL ac-tivity but do not appear to be associated with increases in lignin O -methyltransferase activities.The Arabidopsis transparent testa glabra1-1 ( ttg1-1 ) mu-tation blocks anthocyanin accumulation and trichome for-mation (Koornneef, 1981). TTG1 encodes a WD40 repeat protein homolog ous with an AN11-encoded protein from petunia, which also controls anthocyanin production (deVetten et al., 1997; Walker et al., 1999). To test the geneticFigure 3.Effect of pap1-D Mutation on Accumulation of Phenylpropanoid Products.(A) to (F) HPLC profiles of phenylpropanoid metabolites in extracts from wild-type Col-0 ([A], [C], and [E]) and pap1-D ([B], [D], [F]) plants.(A) and (B) Soluble phenolics: peak 1, rhamnose (Rha)-Glc-Rha-quercetin; peak 2, quercetin conjugate; peak 3, Rha-Glc-Rha-kaempferol; peak 4, Glu-Rha-quercetin; peak 5, Rha-Rha-quercetin; peak 6, Glc-Rha-kaempferol; peaks 7 and 8, kaempferol conjugates; peak 9, sinapic acid;peak 10, Rha-Rha-kaempferol.(C) and (D) Anthocyanidins; the inset in (D) shows the UV light absorption spectrum of the major anthocyanidin eluting at 21.5 min.(E) and (F) Wall-bound phenolics: peak 1, trans -4-coumaric acid; peak 2, sinapic acid; peak 3, cis -4-coumaric acid.Activation Tagging of PAP12387relationship between TTG1 and PAP1, we crossed ttg1-1 with pap1-D. The pap1-D allele was tracked by Basta resis-tance, and the ttg1-1 mutation was scored visibly. The dou-ble mutant F2 plants failed to accumulate anthocyanins, indicating that TTG1 is required for the production of antho-cyanins mediated by PAP1 overexpression and acts either downstream from or at the same step as PAP1.Cloning of PAP1In a population of Ͼ100 seg reg ating T2 plants, each plant that had the pap1-D phenotype showed resistance to Basta, and all plants with a wild-type phenotype (i.e., lacking purple pig mentation) were sensitive to Basta, indicating that the mutation was tightly linked to the T-DNA insert. Moreover, hybridization of DNA gel blots of pap1-D genomic DNA that had been digested with EcoRI or KpnI showed that the 35Se sequences were localized to single fragments of 10 and 12 kb, respectively (data not shown), indicating that the mutant contained a single, simple insertion. The 12-kb KpnI and 10-kb EcoRI fragments were cloned by plasmid rescue (Weigel et al., 2000), g enerating pPAP1-K1 and pPAP1-E1, respec-tively. Nucleotide sequencing and restriction analysis showed that the 12-kb KpnI frag ment fully overlapped the smaller EcoRI frag ment. Probing the Arabidopsis CD4-7 cDNA li-brary at high stringency with pPAP1-K1 resulted in isolation of a single 956-bp cDNA, which defined three exons in the genomic DNA of pPAP1-K1 encoding an MYB transcription factor (Figures 4A and 4B). In the pap1-D line, the 35Se se-quences had inserted 5.1 kb 3Ј to the start of this gene, des-ignated PAP1, and RNA gel blot hybridization with the PAP1 cDNA revealed a sing le 1-kb transcript, which was mas-sively overexpressed in the pap1-D mutant (Figure 4C).To confirm that overexpression of PAP1 caused the pap1-D phenotype, we cloned a 3-kb g enomic frag ment spanning the three PAP1 exons and flanking sequences into pMN20-2, which contains two copies of 35Se (Weig el et al., 2000), thereby creating pMN-PAP1. Transformation of wild-type Col-0 with this construct, which mimics the g enomic con-text of the pap1-D allele, generated multiple transgenic lines with the characteristic purple phenotype (Fig ure 5B). As would be expected from position effects, these transgenic lines represented an allelic series ranging from the wild type to an even more intense phenotype than pap1-D, in some cases having strong purple pigmentation in the petals (Fig-ure 5E). In contrast, transformation with pMN20-2 as an empty vector control gave no enhanced pigmentation phe-notype (Figures 5A and 5D).Sequence alig nments with the Arabidopsis databases showed that PAP1 is a member of the R2, R3 MYB family, which is estimated to have Ͼ100 members in Arabidopsis (Kranz et al., 1998; Romero et al., 1998) and Ͼ80 membersin maize (Rabinowicz et al., 1999). PAP1 is identical to ATMYB75 (Kranz et al., 1998) except that ATMYB75 con-tains a sequencing error (1-bp deletion at position 695), cre-ating an early stop codon. The PAP1 protein shares hig h homology with other MYB-like transcription factors that reg-ulate anthocyanin production (Figures 4B and 4D). PAP1 is closely related to the product of the petunia AN2 g ene, showing 82% identity throug h the MYB reg ion and 50% identity overall. The products of the maize anthocyanin MYB genes C1 and pl are 64% identical through the MYB region, with 38% identity overall. The MYB transcription factor GLABAROUS1 from Arabidopsis and MIXTA from snap-dragon both control trichome development (Oppenheimer et al., 1991; Glover et al., 1998) and are each 58% identical to PAP1 in the MYB domain and 33% identical overall. A phy-log enetic tree constructed with these full-leng th MYB pro-teins shows that PAP1 belong s to a branch involved in anthocyanin biosynthesis (Figure 4D).The PAP1 gene was mapped to 0.2 centimorgan (cM) up from mi303 at 83.7 cM on chromosome 1 by using an Xba1 restriction frag ment leng th polymorphism and the Col/Ler recombinant inbred lines (Nottingham University Stock Cen-tre, Nottingham, UK). The sequencing project recently came to PAP1 on bacterial artificial chromosome F25P12 just be-low mi303 at 85 cM.PAP2Also discovered in the Arabidopsis database was 193M15, an expressed sequence tag with very hig h homolog y withTable 1.Enhanced PAL Activity and Lignin Levels in pap1-D PlantsPlant PAL Activity(mkat/g FW)aStemCOMT b Activity(kat/g FW)StemCCOMT c Activity(pkat/g FW)StemLignin CompostionTotal G(mol/g)Total S(mol/g)Total G and S(mol/g)S/GWild type30.544.250.421.6 2.524.10.12 pap1-D70.344.460.630.6 5.035.60.16a FW, fresh weight; kat, katal.b COMT, caffeic acid O-methyltransferase.c CCOMT, caffeoyl-CoA O-methyltransferase.2388The Plant CellFigure 4.Molecular Characterization of PAP1.Activation Tagging of PAP12389PAP1. 193M15 encodes a protein with 93% identity to PAP1 in the MYB domain and 77% identity overall. To test whether overexpression of 193M15 also could cause the production of anthocyanin pig ments, we made a 35S pro-moter::cDNA fusion construct, pCHF3-PAP2. Transgenic plants containing pCHF3-PAP2 had purple leaves and stems (Fig-ure 5C), althoug h the pig mentation was somewhat weaker than that of the pap1-D mutant or some pMN-PAP1 lines. In view of the sequence similarity between the PAP1 protein and that encoded by 193M15 (Figures 4B and 4D) and similar overexpression phenotypes (Fig ure 5C), we named the 193M15 cDNA PAP2. PAP2 is on bacterial artificial chromo-some T27F4 near mi424 on chromosome 1, ف9 cM below PAP1. PAP2 is also a member of the Arabidopsis MYB family reported as ATMYB90 (Kranz et al., 1998).Overexpression of PAP1 and PAP2 in TobaccoTo test whether PAP1 and PAP2 could enhance pigmenta-tion in other plants, we transformed tobacco cv xanthi with pCHF3-PAP1 and pCHFS-PAP2. Both constructs g ener-ated purple tobacco plants, indicating that the Arabidopsis PAP1 and PAP2 genes could activate production of antho-cyanin pig ments in another species (Fig ures 5G and 5H). These transgenic tobacco plants also produced flowers with much more pig mentation than did the pCHF3 transg enic control plants (Figures 5I and 5J). Tobacco transformed with pCHF3 as an empty vector control did not have an in-creased pigmentation phenotype (Figures 5F and 5I). DISCUSSIONAccumulation of phenylpropanoid products during develop-ment is under tight transcriptional regulation, and the pap1-D phenotype represents a striking override of these g enetic controls. Thus, specific tranches of the overall pathway ap-pear to be controlled by separate sets of transcription fac-tors. For example, the maize myb g enes C1 and pl are involved in the reg ulation of anthocyanin synthesis from CHS onward but do not regulate PAL and other genes of the upstream central pathway (Cone et al., 1993a, 1993b; Mol et al., 1996), whereas P independently controls the 3-deoxy flavonoid branch pathway (Grotewold et al., 1994). In con-trast, the pap1-D phenotype, which results from overex-pression of the PAP1 MYB transcription factor, reflects massively enhanced expression of PAL as well as CHS, the g ene encoding dihydroflavonol reductase, and the g lu-tathione S-transferase gene. This broad transcriptional acti-vation of the overall pathway is accompanied by a corresponding ly broad pattern of enhanced product accu-mulation with increases in lig nin, wall-bound hydroxycin-namic acid esters, flavonols, and anthocyanins. Moreover, pathway activation in pap1-D was observed in all vegetative organs and maintained throughout development, in marked contrast to activation in wild-type plants of individual branch pathways at defined developmental stages and with charac-teristic cell-type, tissue-type, and organ specificities (Graham, 1991; Grotewold et al., 1994). The relatively modest in-crease in lig nin content probably reflects a major control point at the polymerization stag e (Bate et al., 1994) with consequent spillover of lig nin monomers and their precur-sors, which contributes to the marked accumulation of wall-bound hydroxycinnamic acid esters in pap1-D.MYB genes contribute to the control of flavonoid biosyn-thesis in a wide range of plant species, often in combination with other regulatory genes. The extensive sequence simi-larity with AN2, c1, and pl, together with the overexpression phenotypes, suggests that PAP1 and PAP2 may be the Ara-bidopsis orthologs of these petunia and maize myb genes, with genetically defined functions in phenylpropanoid regu-lation. In maize, c1 and pl but not P require R and B, encod-ing basic helix-loop-helix proteins, to activate transcription of maize flavonoid biosynthetic genes (Mol et al., 1996). Ba-sic helix-loop-helix proteins and MYB proteins also function together in the control of flower pigmentation in snapdragon (Goodrich et al., 1992) and petunia (Quattrocchio et al., 1998). Moreover, the WD40 proteins TTG1 and AN11 are re-quired for MYB control of flavonoid biosynthesis in bothFigure 4.(continued).(A) Genomic context of T-DNA insertion in pap1-D and structure of pMN-PAP1. Basta r, Basta resistance; Kan r, Kanamycin resistance; LB, left border; pBS, pBluescript KSϩ plasmid; RB, right border; 4 ϫ 35S denotes four copies of 35Se.(B) Sequence homology of R2R3 MYB. Proteins were aligned using the ClustalW software program. Red shading denotes 100% conserved res-idues, and yellow shading denotes matching residues with PAP1. R2 and R3 MYB domains are shown. GenBank accession numbers PAP1 (AF325123), PAP2 (AF325124), AN2 (AAF66727), C1 (AAA33482), P1 (AAB67720), P (AAC49394), GL1 (P27900), Mixta (CAA55725), and c-myb (AAA52031).(C) Gel blot hybridization of total RNA from 4-week-old pap1-D and Col-0 plants.1.0 denotes transcript size in kilobases.(D) Neighbor-joining phylogenetic tree built by using full-length proteins, showing the anthocyanin MYB family branch. The human gene c-myb is used as an outgroup.2390The Plant CellArabidopsis and petunia (de Vetten et al., 1997; Larkin et al.,1999; Walker et al., 1999), and the pap1-D phenotypes re-quire the WD40 gene TTG1. Despite the stringent and often complex g enetic control of phenylpropanoid biosynthesis,strong overexpression of PAP1 in the pap1-D line was suffi-cient to hyperactivate the pathway, which is reminiscent of the enhancement of flavonoid biosynthesis by deliberate ectopic expression of P in suspension cultures of maize cells (Grotewold et al., 1999). The pap1-D phenotypes may reflect involvement of PAP1 as the limiting factor in a novel reg ulatory circuit with atypically broad control functions in phenylpropanoid biosynthesis or may indicate functional spillover from one regulatory circuit to related circuits when PAP1 is massively overexpressed.A recent report describes the use of activation tagging in Catharanthus cell cultures to isolate ORCA3, a jasmonate-responsive transcriptional regulator of primary and second-ary metabolism, the upregulation of which promotes biosyn-thesis of indole alkaloids (Van der Fits and Memelink, 2000).These data, along with the present finding s, indicate that activation tag g ing can be used to g enerate novel g ain-of-function mutations that affect complex biosynthetic path-ways under polygenic control; as such, this presents a po-tentially powerful new approach for isolating the genes that reg ulate biosynthesis of plant natural products. Loss-of-function screens for transparent testa have been saturated,and no mutations map to the PAP1 or PAP2 loci (Shirley et al., 1995). Moreover, examination of Ͼ100 PAP1 antisense lines showed no visible phenotype (data not shown). The similar overexpression phenotypes of PAP1 and PAP2 sug-gest that these genes may be functionally redundant, such that only activation tagging or some other gain-of-function screen could have readily revealed their key attributes.Activation tag g ing as a g ene discovery tool based on g ain-of-function is intrinsically oriented toward biotechno-logical utility, and the ability to activate a biosynthetic path-way that will lead to the enhanced accumulation of several distinct subclasses of natural products has several impor-tant potential applications. Thus, hyperactivated tissues or org ans provide massively enriched sources for passag e through multiplex drug screens with the potential for discov-ery of novel activities based on what combinatorial effectsFigure 5.Overexpression of PAP1 or PAP2 Enhances Pigmentation in Arabidopsis and Tobacco.(A) to (E) Arabidopsis plants transformed with pMN20-2 ([A] and [D]), pMN-PAP1 ([B] and [E]), and pCHF3:PAP2 (C). (A) to (C) show six-week-old plants. (D) and (E) show flowers on 12-week-old plants.(F) to (J) Tobacco plants transformed with pCHF3 ([F] and [I]), pCHF3:PAP1 (G), and pCHF3:PAP2 ([H] and [J]). Plantlets in (F) to (H) were pho-tographed at age 4 weeks, and flowers in (I) and (J) at 10 weeks after transfer to soil. pCHF3-PAP1 plants had brilliant flower pigmentation, iden-tical to that of pCHF3-PAP2 (data not shown).Activation Tagging of PAP12391might arise from complex mixtures as well as allowing con-venient isolation and characterization of individual bioactive components. This approach in principle could be aug-mented by feeding studies using pathway intermediates or synthetic derivatives. Moreover, activation of phenylpro-panoid biosynthesis in pap1-D reflects massively enhanced expression of g enes encoding pathway enzymes; hence, these tissues provide a correspondingly enriched source for isolating the cDNAs that encode key biosynthetic enzymes not readily identified by biochemical approaches.Although the plant kingdom has a remarkable diversity of natural products, the underlying pathway regulatory mecha-nisms appear to be at least partially conserved between species (Mol et al., 1996; Quattrocchio et al., 1998). For ex-ample, the maize anthocyanin reg ulatory g ene R functions appropriately when expressed in Arabidopsis or tobacco (Lloyd et al., 1992). Likewise, ectopic expression of PAP1 or PAP2 in transg enic tobacco caused phenotypes similar to those observed in Arabidopsis. Therefore, convenient, readily transformed genetic model species, such as Arabi-dopsis, can be used to isolate candidate regulatory genes for direct evaluation in medicinal plants and other exotic species or as a platform for the identification of ortholog s and potentially useful, related genes in target species.The serendipitous discovery of pap1-D among a larg e collection of activation-tag g ed lines was possible because activation of PAP1 enhanced the accumulation of anthocya-nin pigments, which was easily scored. Several other plant natural products, such as the isoprenoids lycopene and car-otene and the alkaloid sang uinarine, also are colored. Hence, genetic activation of these tranches of plant metab-olism also could be scored by visual inspection, but this is not a generally applicable approach. In principle, activation-tag g ed lines with enhanced accumulation of natural prod-ucts of interest could be identified by high-throughput meta-bolic profiling. However, a more promising general strategy may be to make transg enic plants that express easily screened marker genes under the control of promoters from g enes encoding enzymes involved in the biosynthesis of natural products of interest.METHODSPlant Growth and TransformationArabidopsis thaliana ecotype Columbia (Col-0) plants were grown in growth rooms at 22ЊC in long days (16 hr of light) or short days (9 hr of light) and received 250 E from three 35-W cool white bulbs and one 35-W Sylvania GrowLux bulb (Osram Sylvania, Danvers, MA). Nico tiana tabacum cv xanthi plants were reg enerated under 24-hr-light conditions at 25ЊC and then transferred to the greenhouse. Tobacco and Arabidopsis transformation was performed as previ-ously described (Neff et al., 1999; Weig el et al., 2000) except that 0.02% Silwet-L77 (Lehle Seeds, Round Rock, TX) was used for the latter. Basta was obtained from AgrEvo (Montvale, NJ).Gel Blot HybridizationDNA and RNA gel blot hybridizations were performed according to standard procedures (Sambrook et al., 1989). RNA samples used in the gel blot analysis shown in Figure 2 were from vegetative leaves of pap1-D and Col-0 plants g rown under short-day conditions for 4 weeks and long-day conditions for 2 weeks. Probes were full-length cDNA fragments of PAP1, the glutathione S-transferase gene, and the g ene encoding ubiquitin. The chalcone synthase (CHS) probe was a polymerase chain reaction product amplified by using the primers 5Ј-TGGTCTCCGTCCTTCCGTCAA and 5Ј-CCCTCAAATGTCCGT-CTATGGAA. The phenylalanine ammonia-lyase (PAL) probe was am-plified by using the primers 5Ј-CTATACGCTTACCTACCAACAAAC and 5Ј-TCTCCGATGAGAAGTAGCACCAA, and the dihydroflavonol reductase probe was amplified with primers 5Ј-AAAAAGATGACA-GGATGGGT-3Ј and 5Ј-CCCCTGTTTCTGTCTTGTTA-3Ј.Enzyme AssaysPAL activity was measured by using a microcuvette spectrophoto-metric assay (Blount et al., 2000). Caffeic acid O-methyltransferase and caffeoyl-CoA O-methyltransferase activities were assayed by standard methods (Inoue et al., 1998). Protein concentrations were determined by the procedure of Bradford (1976).Phenylpropanoid AnalysisSoluble and wall-bound phenolics in whole-plant extracts as well as extracts of individual tissues were analyzed by HPLC (Blount et al., 2000). The aqueous phase, which remained after ethyl acetate ex-traction of the wall-bound phenolics, was lyophilized and resus-pended in 70% methanol for analysis. The HPLC eluates were monitored by absorbance at 270, 310, and 550 nm, and the peaks were identified by comparing their retention times and UV light spec-tra with those of known standards. Lignin was assayed by derivatiza-tion followed by reductive cleavage (Lu and Ralph, 1997).pMN-PAP1, pCHFS-PAP1, and pCHFS-PAP2 ConstructsA PAP1 genomic fragment was amplified by using 5Ј-AACTACTGC-AGCTAGAGCGTAGAGG-3Ј and 5Ј-TCAAACTGCAGAAACTAAGCC-CA-3Ј to construct 5Ј and 3Ј Pst sites. This fragment was cloned into pMN20-2 (Weigel et al., 2000), which contains two copies of 35Se to create pMN-PAP1. PCHF3-PAP1 was created by amplifying the PAP1 cDNA with primers 5Ј-ACTGGTACCTTTTACAATTTGTTTA-3Јand 3Ј-AAGGGATCCTATACACAAACGCA-5Ј and cloning it into the KpnI and BamHI sites of pCHF3, a pPZP211-based plant expression vector carrying the cauliflower mosaic virus 35S promoter and a pea ribulose 1,5-bisphosphate carboxylase/oxyg enase terminator (C. Fankhauser, K. Hanson, and J. Chory, unpublished data). The PAP2 cDNA was excised from expressed sequence tag clone 193M15 with KpnI and BamHI and was cloned into pCHF3 to create pCHF3-PAP2.ACKNOWLEDGMENTSWe thank the following (all at Salk Institute unless otherwise noted): Tseg aye Dabi for help with transg enic tobacco; Mary Anderson at。
语言学导论知到章节答案智慧树2023年潍坊学院

语言学导论知到章节测试答案智慧树2023年最新潍坊学院第一章测试1.Which of the following property of language enables language users toovercome the barriers caused bytime and place, due to this feature oflanguage, speakers of a language are free to talk about anything in anysituation? ()参考答案:Transferability2.Duality is one of the characteristics of human language. It refers to the factthat Language has two levels of structures: the system of sounds and thesystem of meanings. ()参考答案:对3.The informative function is predominantly the major role of language, it isalso called _________ function in the framework of functional grammar. ()参考答案:ideational4.According to F. de Saussure, _____ refers to the abstract linguistic systemshared by all the members of a speech community. ()参考答案:Languengue is relatively stable and systematic while parole is subject to personaland situational constraints. ()参考答案:对6._____ is realized by mood and modality.()参考答案:Interpersonal function7.Which of the following statements is true of Jacobson’s framework o flanguage functions?()参考答案:The phatic function is to establish communion with others8.Onomatopoeic words can show the arbitrary nature of language. ()参考答案:错petence and performance refer respectively to a language user’sunderlying knowledge about the system of rules and the actual use oflanguage in concrete situations. ()参考答案:对10.Arbitrariness of language makes it potentially creative, and conventionalityof language makes a language be passed from generation to generation. As a foreign language learner, the latter is important for us. ()参考答案:对第二章测试1.Of the consonants /p/, /t/, /k/, /f/, /m/, /z/ and /g/, which has the featuresof voiceless and velar? ()参考答案:/k/2. A sound which is capable of distinguishing one word or one shape of wordfrom another in a given language is a _______. ()参考答案:phoneme3.Which of the following CANNOT be considered as minimal pair? ()参考答案:/s/-/θ/4._______ is one of the suprasegmental features.()参考答案:Tone5.Classification of English speech sounds in terms of manner of articulationinvolves the following EXCEPT_______.()参考答案:Bilabial6.An aspirated p, an unaspirated p and an unreleased p are _______ of the pphoneme.()参考答案:Allophones7.Conventionally a_______ is put in slashes. ()参考答案:phoneme8.Which one is different from the others according to manners of articulation?()参考答案:[w]9.Which one is different from the others according to places of articulation? ()参考答案:[n]10.Which of the following is the correct description of [v]? ()参考答案:voiced labiodental fricative第三章测试1.In terms of______ , words can be divided into closed-class words and open-class words.()参考答案:membership2.Words like pronouns, prepositions, conjunctions, articles are ______ items. ()参考答案:closed-class3.Bound morphemes do not include. ()参考答案:Words4.______ refers to the way in which a particular verb changes for tense, person,or number. ()参考答案:Inflection5.Which two terms can best describe the following pairs of words: table—tables, day+break—daybreak. ()参考答案:inflection and compound6.Which of the following words are formed by blending? ()参考答案:Smog7. A prefix is an affix which appears ______.()参考答案:before the stem8.Nouns, verbs, adjectives and many adverbs are content words. ()参考答案:对9.Pronouns, prepositions, conjunctions and articles are all open class items. ()参考答案:错10.Free morpheme may constitute words by themselves.()参考答案:对第四章测试1._______ refer to the relationship that linguistic units have with other unitsbecause they may occur together in a sentence. ()参考答案:Syntagmatic relations2.The phrase “on the shelf” belongs to _______ construction.()参考答案:exocentric3.______refers to construction where one clause is coordinated or conjoinedwith another. ()参考答案:Conjoining4.IC analysis can be used to analyze all kinds of ambiguous structures.()参考答案:错5.__________ is concerned with the internal organization of words.()参考答案:Syntax6.Transformational Generative Grammar was introduced by_______in 1957.()参考答案:N·Chomsky7.Predication analysis is a way to analyze _______ meaning. ()参考答案:sentence8. A sentence is considered_______ when it does not conform to the grammaticalknowledge in the mind of native speakers. ()参考答案:wrong9.Phrase structure rules allow us to better understand_______. ()参考答案:all of the other answers10.Phrase structure rules have properties. ()参考答案:Recursive第五章测试1.“ We shall know a word by the company it keeps.” This statementrepresents___.()参考答案:contexutalism2._______is a way in which the meaning of a word can be dissected into meaningcomponents, called semantic features.()参考答案:Componential analysis3.The grammaticality of a sentence is governed by_______.()参考答案:grammatical rules4.Words which have different meanings but are written differently and soundalike are called hyponyms.()参考答案:错5.In a sentense relation of hyponymy, a superordinate entails all hyponyms. ()参考答案:对6.In componential analysis, _______be analyzed into HUMAN, ADULT and MALE.()参考答案:manponential analysis is a way to analyze _______ meaning. ()参考答案:word8.The relationship between “flower” and“rose” is _______. ()参考答案:hyponymy9.Synonymy is the technical name for the sameness relation. ()参考答案:对10.Converse opposites are pairs of words that exhibit the reveral of arelationship between the two items. ()参考答案:对第六章测试1.Promising, undertaking, vowing are the most typical of the_______. ()参考答案:commissives2.What essentially distinguishes semantics and pragmatics is whether in thestudy of meaning _________ is considereD.()参考答案:context3. A sentence is a _________ concept, and the meaning of a sentence is oftenstudied in isolation. ()参考答案:grammatical4.Which of the following is true?()参考答案:Some utterances cannot be restored to complete sentences.5.According to Searle, the illocutionary point of the representative is ______.()参考答案:to commit the speaker to something’s being the case6.All the acts that belong to the same category share the same purpose, butthey differ _________. ()参考答案:in their strength or force7._________ is advanced by Paul Grice. ()参考答案:Cooperative Principle8.Pragmatics treats the meaning of language as something intrinsic andinherent. ()参考答案:错9.It would be impossible to give an adequate description of meaning if thecontext of language use was left unconsidered.()参考答案:对10.What essentially distinguishes semantics and pragmatics is whether in thestudy of meaning the context of use is considered.()参考答案:对第七章测试1.is defined as any regionally or socially definable human group identified byshared linguistic system. ()参考答案:A speech community2._______is a casual use of language that consists of expressive but nonstandardvocabulary, typically of aibitrary, flashy and often ephemeral coinages andfigures of speech. ()参考答案:Slang3.In a speech community people have something in common_______--a languageor a particular variety of language and rules for using it. ()参考答案:linguistically4._______refers to the use of a word which is thought to be less offensive orunpleasant than another worD.()参考答案:Euphemism5.The most distinguishable linguistic feature of a regional dialect is its_________.()参考答案:accent6._______are the major source of regional variation of language. ()参考答案:Geographical barriers7.Linguist______published his paper “The social stratifi cation of English in NewYork City”in 1966. ()参考答案:Labow8. A regional variety of a language is intrinsically inferior to the standardvariety of that language.()参考答案:错9.The standardization of a particular dialect in relation to one or morevernaculars is the result of a deliberate governmental policy. ()参考答案:错10. A pidgin is not a native language of a particular region.()参考答案:对。
美国通用电气公司的战略管理模式

Work-out Process
Transformation of culture at GE to sustain growth & productivity
Organizational Restructure – “Work-out”
SWOTVisionRestructuringGrowth EngineSustaining Competitive advantageDynamic Perspective
4 blocks
Six Sigma
Inertia
Best practices
Strategic Model
and change
Financial Services
Commercial Finance
Consumer Finance
Equipment Management
Insurance Business
A Summary of Business Finances
The Share of the Pie…
The Share of the Pie…
“Work-out” was a process initiated by Jack Welch and James Baughman to address the issue of increasing productivity by changing the in-house culture to reflect that of an small firm, characterized by Speed Simplicity Self Confidence
工业工程专业英语--翻译

工业工程专业英语--翻译工业工程的真正价值 Real IE ValueIn addition, the IE now has a greater opportunity to concentrate on any one of a broad variety of areas that many companies now recognize as individual departments-including simulation, operations research, ergonomics, material handling and logistics.值得一提的是,工业工程现在有更多的机会去集中于现在许多企业已经视为独立的学科的众多领域中的一个-----包括防真学、运筹学、人因学、物料搬运和物流学。
Work-measured Labor Standards 基于作业测量的劳动标准If you are a manufacturer, chances are you have a bill-of-materials (BOM) system to determine standard parts cost. Do you also have an equivalent bill-of-labor system to determine standard labor cost?如果你是一个制造商,你有可能会有一个物料清单系统来确定标准件的成本。
你是否也能得到类似的劳动力清单系统来确定标准的劳动成本,Time study——The most widely used tool to develop standard times is still time study. Time study reflects what is happening in your job or project. It is also easy to learn and use. Now, the PC has made summarization of time study data a matter of seconds instead of hours.时间研究----用来开发标准时间使用最广泛的工具依然是时间研究。
高顿财经ACCA-模考卷-F1

Accountant in BusinessMock ExaminationSECTION AALL 46 questions are compulsory and MUST be attempted1. Which of the following is a responsibility of a mentor? A. To show a trainee how to perform tasks B. To be a role model for an employeeC. To help others to explore their own problems(1 mark)2. ........................................ taxes are collected by the Revenue authority from a business, which attempts to pass on the tax to consumers in the price of goods. Which word correctly completes this statement? A. Progressive B. Direct C. Indirect(1 mark)3. The finance director of JLX Co is an ACCA member. He has been asked to pay a bribe to secure a contract in a country in which bribery is commonplace. Which of the following statement is correct?A. The bribe is unacceptable because a professional accountant should not behave in an unethical wayB. The bribe should be paid because it is in the interests of the companyC. The bribe is acceptable because it is normal business practice in the country(1 mark)4. Charles, an accountant, suspects Lin, a client, of money laundering. By warning Lin that he intends making a report to the appropriate authority, Charles is committing an offence. Is the statement true or false? A. FALSE B. TRUE(1 mark)5. Which TWO of the following are characteristics of a relativist approach to ethics? A. What is right in one situation may not be right in another B. Ethics are linked to cultural beliefsC. There is a set of moral rules which are always right(1 mark)6. The meaning of a message can be lost at the coding and decoding stages. What is the name given to this process? A. Distortion B. Noise C. Feedback(1 mark)高顿财经A CC A7. Non-executive directors should have no business, financial or other connection with the company, apart from fees and shareholdings.Is this statement true or false? A. TRUE B. FALSE(1 mark)8. Which of the following brings together individuals with different competencies and specialisms? A. Functional teams B. Virtual teamsC. Multi-disciplinary teams(1 mark)9. In the context of data security controls, ........................................ are records showing who has accessed a computer system and what operations he or she has performed. Which word or phrase correctly completes this definition? A. Passwords B. Audit trails C. Archives(1 mark)10. Which of the following are responsible for taking positive steps to detect and prevent fraud in a company? A. The external auditors B. The executive directors C. The shareholders(1 mark)11. Positive discrimination is the process of taking active steps to encourage people from disadvantaged groups to apply for jobs and training and to compete for vacancies. Is the above statement true or false? A. TRUE B. FALSE(1 mark)12. Do personal development plans incorporate a wide set of developmental opportunities? A. Yes B. No(1 mark)13. The executives of BBB Company have observed that their colleagues in country A are aggressive in the pursuit of their targets, highly competitive with one another and focused on the material rewards of their work. By contrast, their colleagues in country B are more team orientated, protective of one another and focused on achieving goals to the mutual benefit of all.These observations relate to which of Hofstede ’s cultural dimensions? A. Power-distanceB. Uncertainty-avoidanceC. Masculinity-femininity(1 mark)高顿财经A CC A14. What is the term given to a method of fraud in the accounts receivable area, by which cash or cheque receipts are stolen, and the theft concealed by setting subsequent receipts against the outstanding debt? A. CollusionB. MisrepresentationC. Teeming and lading(1 mark)15. Job evaluation puts a relative value on jobs primarily on the basis of which of the following factors? A. EquityB. Job contentC. Market rates of pay(1 mark)16. Which of the following is not a purpose of performance appraisal? A. Job evaluationB. Identification of training needsC. Succession planning(1 mark)17. A company has advertised for staff who must be at least 1·88 metres tall and have been in continuous full-time employment for at least five years.Which of the following is the legal term for this practice? A. Indirect discrimination B. Direct discrimination C. VictimisationD. Implied discrimination(2 mark)18. Which of the following organisations would be best able to adopt a wide span of control in its structure? A. A factory in which many workers are employed to carry out homogeneous tasks B. A university offering degree programmes in all arts and science disciplinesC. A call centre in which multi-skilled workers are employed to carry out a wide variety of tasksD. A hospital offering general care, outpatient services and accident and emergency services(2 mark)19. Janet is a member of a project group. She is highly knowledgeable and considered by her colleagues to be rather intellectual and strategic in her approach. Janet demonstrates great intuition when dealing with problems sometimes adopting unorthodox but effective methods. However some of the team regard her as detached and uncommunicative, and she can sometimes disregard practical details without explanation. According to Belbin's team roles theory Janet is an example of which of the following? A. PlantB. Monitor-evaluatorC. Resource investigatorD. Shaper(2 mark)20. Which TWO of the following are examples of connected stakeholders? A. Executive directors B. Employees C. LendersD. Shareholders(2 mark)高顿财经A CC A21. The research and development (R & D) function of a business: (i) is primarily concerned with market research(ii) can improve existing products as well as developing completely new products(iii) has been less important for firms manufacturing computers to meet an industry standard than for those firms developing the next generation of computers(iv) is always undertaken under contract by specialist external consultancies Which of the above statements are correct? A. (i) and (ii) only B. (ii) and (iii) only C. (i), (iii) and (iv) only D. (ii) and (iv) only(2 mark)22. Which of the following supports the marketing function by analysing and projecting the costs and revenues of a new product and assessing the overall implications of introducing the product for the organisation? A. Financial controller B. Financial accountant C. Management accountant D. Treasury manager(2 mark)23. Which of the following should take place first when implementing a performance appraisal system? A. Setting the criteria for assessmentB. Agreeing on how performance and rewards will be linkedC. Deciding on a common approach to appraisal interviewsD. Training managers in effective interpersonal skills to be used in appraisal interviews(2 mark)24. Frida has attended a personal development programme on which she participated in an exercise based on the Blake and Mouton managerial grid. One conclusion of the exercise is that she demonstrates a '5.5' leadership style.What does the outcome of the exercise demonstrate?A. Frida is a team player and achieves a high level of harmony in the working group sometimes at the expenseof achieving resultsB. Frida balances the need to get the job done with the need to maintain the teamC. Frida optimises the effectiveness of her team by achieving the highest level of morale and taskaccomplishmentD. Frida is entirely focused on getting the job done and pays minimal attention to team maintenance needs(2 mark)25. Which of the following statements is correct in relation to the informal organisation?A. Communications through the informal organisation will always be slower than through formalcommunication channelsB. Rumours do not circulate through the informal organisationC. The informal organisation has only a negative effect on the organisation and its objectivesD. Individuals may communicate horizontally more effectively through the informal organization(2 mark)高顿财经A CC A26. BCD Co is a large trading company. Steve is the administration manager and is also responsible for legal and compliance functions. Sheila is responsible for after sales service and has responsibility for ensuring that customers who have purchased goods from BCD Co are fully satisfied. Sunny deals with suppliers and negotiates on the price and quality of inventory. He is also responsible for identifying the most appropriate suppliers of plant and machinery for the factory. Sam is the information technology manager and is responsible for all information systems within the company.According to Porter’s value chain, which of the managers is involved in a primary activity as opposed to a support activity? A. Steve B. Sheila C. Sunny D. Sam(2 mark)27. Which of the following are ‘on the job’ training methods? (i) Day-release (ii) Job rotation (iii) Coaching(iv) Temporary promotion A. Method (iii) onlyB. Methods (i) and (iii) onlyC. Methods (ii), (iii) and (iv) onlyD. Methods (i), (ii), (iii) and (iv)(2 mark)28. Martin is an experienced and fully trained shipbuilder, based in a western European city. Due to significant economic change in supply and demand conditions for shipbuilding in Martin’s own country, the shipyard he worked for has closed and he was made redundant. There was no other local demand for his skills within his own region and he would have to move to another country to obtain a similar employment, and could only find similar work locally through undertaking at least a year’s retraining in a related engineering field. Which of the following describes the type of unemployment that Martin has been affected by? A. Structural unemployment B. Cyclical unemployment C. Frictional unemployment D. Marginal unemployment(2 mark)29. Which of the following is not a technique of scientific management or Taylorism? A. Micro-design of jobsB. Work study techniques to establish efficient methodsC. Multi-skilled team workingD. Financial incentives(2 mark)高顿财经A CC A30. The finance director of a company is concerned that the company will not have sufficient cash available at the end of the month to pay the staff's wages unless he arranges an overdraft with the bank.Which of the following will be most useful to the finance director in working out whether he needs to arrange the overdraft?A. The most recent statement of profit or lossB. The statement of financial position at the end of the previous monthC. The current month's cash flow forecastD. The variance reports from the end of the previous month(2 mark)31. A government has forecast the following future trends. Which TWO of these are social and demographic trends?A. Increased demand for organic and environmentally friendly productsB. Significant increases in single person householdsC. Increased revenues from personal taxationD. Rapid increases in household incomes(2 mark)32. Peter is a newly appointed chief executive officer of a health organization. He finds that financial resources are insufficient meet demand for health care.Which of the following courses of action is the MOST appropriate in Peter ’s position?A. Establish a system which determines health care priorities and delivers health care according to thesepriorities within budgetB. Ignore the financial restrictions and continue to treat all patients according to needC. Treat all patients until financial resources are exhausted and then cease to provide health careD. Report information to a local newspaper and ask them to petition for increased resources(2 mark)33. Which TWO of the following are performed by procurement department in a business? A. Obtaining price quotationsB. Authorising customers ’ credit limitsC. Producing sales ordersD. Producing purchase orders(2 mark)34. Arthur, Brian, Carol, and Diane all work in the finance department of TBC Co. Arthur is responsible for maintaining the cash book, Brian prepares monthly budgets, Carol monitors internal control systems and Diane manages surplus funds.Which member of staff has a financial accounting role? A. Diane B. Arthur C. Brian D. Carol(2 mark)35. In relation to Maslow ’s hierarchy of needs, in which order are the following needs fulfilled? (1) Esteem (2) Safety (3) Social A. 1,2,3 B. 2,1,3 C. 2,3,1 D. 3,1,2高顿财经A CC A(2 mark)36. Which of the following describes the agency relationship in business? A. Stakeholders are the agents of managersB. Managers must accept the objectives set by stakeholdersC. Managers must consider the view of stakeholders when setting objectivesD. Stakeholders have no influence over business objectives(2 mark)37. Which TWO of the following statements are true? A. A manager should prioritise tasks B. Job analysis aids good managementC. Delegation is a sign of weak managementD. All goals must be expressed as quantities(2 mark)38. An organization has many layers, a narrow span of control and there is a presumption of logic and rationality. The organization is very large and operates in a stable competitive and regulatory environment. People and processes within the organization operate strictly by well established rules and procedures. Which cultural type of organization is being described in the above statement? A. Person culture B. Role culture C. Task culture D. Power culture(2 mark)39. Which of the following is a disadvantage of a large panel or selection board interview compared to individual or one-to-one interviews?A. A number of people see the candidate at one sittingB. Specialists can ask a questions about technical areas of the workC. Questions tend to be more varied and more randomD. There is less opportunity for personal rapport with the candidate(2 mark)40. Which TWO of the following are recommended as best practice with regard to remuneration committees? A. They should be staffed by executive directors who know about the day running of the business B. They should have no related interests other than as shareholdersC. They should determine specific remuneration package as well as general policy(2 mark)41. The currency in country X is the Krone while country Y uses the Euro. Country Y has recently experienced an increase in its exchange rate with Country X. Which of the following effects is likely to result in Country Y? A A stimulus to exports in Country YB An increase in the costs of imports from Country XC Reducing demand for imports from Country XD A reduction in the rate of cost push inflation(2 mark)高顿财经A CC A42. Which one of the following would cause the supply curve for a good to shift to the right (outwards from the origin)?A A fall in the price of the goodB An increase in the demand for the goodC A fall in production costs of the goodD The imposition of a minimum price(2 mark)43. If the absolute value of the price elasticity of demand for dry white wine is greater than one, a decrease in the price of all wine would result in:A A more than proportional decrease in the quantity of dry white wine purchasedB A less than proportional decrease in the quantity of dry white wine purchasedC A less than proportional increase in the quantity of dry white wine purchasedD A more than proportional increase in the quantity of dry white wine purchased(2 mark)44. Which of the following terms is not used by Mintzberg in his description of organisational structure? A Strategic apex B Support base C Technostructure D Operating core(2 mark)45. Some controls are provided automatically by the system and cannot be by-passed, ignored or overridden: for example, having to input a password to enter a computer system. These are classified as........................................ controls.Which term correctly completes this statement? A Detect B MandatedC Non-discretionaryD Administrative(2 mark)46. You have been asked to work on a major investment decision that your company will be making, and discover that your brother-in-law is the managing director of a firm that may benefit from the outcome of the decision. You have no intention of allowing this to influence the advice you give your firm, and you know that your brother-in-law will not try to influence you in any way.What professional quality would make you consider handing this task to a colleague, or otherwise raising questions with your superiors? A Scepticism B AccountabilityC Independence of mindD Independence in appearance(2 mark)高顿财经A CC ASECTION BALL SIX questions are compulsory and MUST be attempted1. The following diagrams represent changes in supply and demand conditions.Diagram A Diagram BDiagram C Diagram D(i)An increase in the costs of factors of production (ii)An increase in the prices of substitute goods(iii)The introduction of a new, more efficient production process(iv)A reduction in incomes of purchasers of the good(b) Indicate which TWO of the following statements are correct.A. If the demand for a product is perfectly elastic, the demand curve is verticalB. An illegal or unofficial market for goods is usually a consequence of an artificially imposed minimum priceC. In the long run, the supply curve is horizontalD. The supply curve for a product is the upward sloping section of the average cost curveE. A change in the price of a product causes a move along the demand curve(2 marks)2. YRT Co is a firm of external auditors which has Company W, Company X, Company Y and Company Z among its audit clients.Company W is currently being sued by one of its suppliers. YRT Co has offered legal services and will defend them in this legal case.The audit partner of Company X has been a member of the external audit team for the past 15 years and has good relationships with the senior management of Company X.The financial controller of Company Y is the daughter of the audit manager from YRT Co.高财CC ACompany Z does not have an experienced financial department and so YRT Co helps to prepare the financial statements of Company Z in advance of the external audit. The following are types of conflicts of interest: A Familiarity threat B Advocacy threat C Self-review threat D Self-interest threat(a) In the case of each of the following clients, which of the above conflicts of interest exists? (i) Company W (ii) Company X (iii) Company Y (iv) Company Z(2 marks)(b) Which of the following would give rise to a threat of intimidation? A Litigation against the external auditor by an audit client B Provision of taxation services as well as audit services C An audit partner is on the board of a client(1 mark)(c) Ethics are a set of that guide behaviour. Which of the following correctly fills the blank? A Legal guidelines B Moral principlesC Professional regulations(1 mark)3. Axel, Bo, Corey and Dagmar work in the finance department of Kett Co.Axel is responsible for summarising confirmed financial information from the nominal ledger and bringing it together to identify annual totals for revenue, expenditure and capital items in order to present finalised accounting information for circulation to shareholders. All of the figures have been checked for accuracy.Bo deals with hundreds of items of income and expenditure each day. Her job is to check the accuracy of the figures if there is any doubt about this. One time consuming aspect of her work is to decide how many sundry items are to be categorised for accounting purposes. This is because those who submit the figures sometimes provide unclear explanations, or no explanations at all, in respect of the income generated or expenditure incurred.Corey is a data input clerk who processes several thousand vouchers each day in order to put them into the accounting system.Dagmar deals with overdue accounts receivable and has to deal with reports from the sales department, categorising each client according to whether they can or cannot receive credit in the future. In serious cases he must decide whether they should be handed to external debt collection agencies.For each of the following functions identify which of the individuals are performing them.(4 marks)高顿财经A CC A. A: Recording financialinformationB: Codifying and processing financial informationC:Preparing financialstatementsAxelBoCoreyDagmar4. Here are four short references to the function of management:A There are essentially five functions of management that apply to any organisationB Individual and group behaviour at work is a major factor in productivity.C Management is the development of a true science of work.D The manager of a business has one basic function – economic performance. Required:(a) Identify the description above which is associated with each of the following theorists, by selecting A, B, C, D or None.(i) Peter Drucker (ii) Elton Mayo(iii) Henry Mintzberg (iv) F W TaylorNote: The total marks will be split equally between each part. (2 marks)(b) Below are listed five functions of management. A Planning B Organising C Managing D Monitoring E Motivating Required:Write down which two of the above are those functions identified by Fayol by selecting TWO of the letters from (A, B, C, D, E).(2 marks)5. Shaun likes to understand basic principles and take a hands-off approach based on logical argument. He wants to participate in a structured training programme which is run by teachers.Louise needs to work at her own pace and tends to be fairly slow and cautious in her learning.She likes to observe things, take time to think about them and then produces a carefully thoughtout conclusion. Susan only likes to study if she can see a link to a real practical problem. She likes to learn new techniquesthrough on-the-job training, but tends to discard good ideas which require some development to work properly. Tony is flexible and optimistic, but does not have any patience with theories. He wants to receive hands-on training and enjoys practical problems.Honey and Mumford classified the four following learning styles:高顿财经A CC AA TheoristsB ReflectorsC ActivistsD Pragmatists(a) For each of the following individuals, select the correct learning style from the list above: (i) Shaun (ii) Louise (iii) Susan (iv) Tony(2 marks)(b) Which TWO of the following are off-the-job training methods? A Job rotationB Computer-based trainingC Temporary promotionD DemonstrationE College courses(2 marks)6. Paul, Mary, Alan and Kate are having a board meeting of a newly formed company and they are discussing the orientation of the company.Paul believes that the company will actively need to persuade customers to buy their products.Mary believes they should add additional features to their products, without carrying out market research, and this will increase demand from customers.Alan believes the products will sell as they are and the company should produce as many items as it can. Kate believes that they should research what customers need and value and adapt the products to meet the findings of the research.The following are types of orientation of a company A Production orientation B Product orientation C Sales orientationD Marketing orientation(a) For each board member, select the orientation they are recommending. (i) Paul (ii) Mary (iii) Alan (iv) Kate(2 marks)(b) Breaking up the market into different groups, which each have common needs, wants and preferences is known asWhich of the options below correctly fills the blank? A Undifferentiated marketing B Marketing mix C Mass marketingD Market segmentation(2 marks)高顿财经A CC A。
HANSEN-汉森减速机简介

Our main product line is the Hansen P4 range of standardized industrial gear units.Low noise and minimum weight are merely a few of its outstanding characteristics which have also been incorporated in the Hansen P4 range of single stage gear units and the Hansen M-Series range of functional drives.Our complete portfolio of products and total drive solutions is reflected in this detailed product brochure.Torsten SchultzManaging Director Business Unit Industryc o n t i n u o u sMoving from customized to serial manufactureled to a number of important benefits:• Production costs were reduced.• Improved quality control procedurescould be implemented.• The number of spare parts was reduced.• Delivery times were slashed.Gear units were never the same again asother manufacturers followed Hansen’s lead.Since then Hansen has consistently been atthe forefront of transmission technology.SizeCentre distanceSpecific housings for horizon-tal and vertical applicationsStandard higher bearing span for vertical applicationsSimple, standard systems for cooling and lubricationAccessories and instrumenta-tion are offered as catalogue optionsImproved component standardizationSpecial ratios up to 1% of the requested ratio at moderate cost Easy to select the optimal gearunit for a specific application -value for moneyStronger shaft support forsurface aerators, mixers...Proven and cost- effectivesolutionsEasy and fast customizationpossibilitiesShorter delivery timesCost efficient tailor-madesolutionRigid, standardized pinionshafts and housingsFull-scale application of:High torque geometry inlow speed gear setsLow noise geometry in highspeed gear setsProfile-controlled gascarburizingPrecision grinding withsophisticated tooth contactanalysisAssured oil circulation throughthe bearingsBetter lateral load distribu-tion, lower noise andvibration levelsBetter strength/surfacedurability rating balanceLower noise and vibrationlevelsOptimal load capacityOptimal transverse and later-al load distribution, lowernoise and vibration levelsLong, troublefree working lifeINSTALLATIONEven the larger Hansen P4 models are easy to transport, manoeuvre and install.■FEATUREProvision for crowbar oradjusting screws, oval transport slots or hoist eyes & standardfixation points for accessories ■YOUR BENEFITEasy installation procedure;easy to handle, install and tomount accessories such asprotection capsSPARE PARTS, SERVICE & MAINTENANCEThe Hansen P4 is one of the easiest to maintain gear units on the market.The maintenance operation itself has been simplified and shortened.■FEATUREHousings for horizontal andvertical applications both withhorizontal splitMaintenance-free Oil-Lock™seal as a standard on the highspeed shaft extensionsDrywell standard for vertically■YOUR BENEFITEasy to open for inspectionand service on-the-spotOil-tight for life -no maintenance requiredOil-tight for life50001,21,251,41,61,822,242,52,83,153,5544.555,6Nominal ratio■FEATURESimple, standard systems forcooling and lubricationAccessories and instrumentation are offered as catalogue options Two mounting positions andalternative mounting with both shafts in a vertical planeLeft hand execution equals right hand executionEnlarged though compact housing and increased shaft height Variable centre distancesImproved component standardization Special ratios up to 1% ofthe requested ratio at moderate cost ■YOUR BENEFITProven and cost- effectivesolutionsEasy and fast customization possibilitiesIncreased flexibility in mount-ing arrangementsEasy change of shaft handing, and only one spare unit need-ed for bothThe input shaft height matchesthe large motor shaft heighteven better Easy replacement andimprovement of existing instal-lations at a minimum effort and costShorter delivery times Cost efficient Tailor-made solution POWER & RELIABILITY■FEATURERigid, standardized pinionshafts and housingsFull-scale application of:High torque geometry in low speed gear setsLow noise geometry in high speed gear setsProfile-controlled gas carburizingPrecision grinding with sophisticated tooth contact analysisAssured oil circulation throughthe bearingsOptimal balance between mechanical, thermal andbearing rating Specific macro gear geometryTapered roller bearings in the monolithic housing Increased shaft height ■YOUR BENEFITBetter lateral load distribu-tion, lower noise and vibration levelsBetter strength/surface durability rating balance Lower noise and vibration levelsOptimal load CapacityOptimal transverse and later-al load distribution, lowernoise and vibration levels Long, troublefree working life Size is equivalent to power, re-sulting in optimal performance and efficiency for all ratios Reduced power losses Lower noise & vibration levels Reduced operating costsIdeal absorbtion of highinternal gear forcesMotor & gear unit equallyaffected by thermal expansion FITNESS FOR USE■FEATURESimple, standard systems for cooling and lubrication Accessories and instrumentation are offered as catalogue options Two mounting positions and alternative mounting with both shafts in a vertical planeLeft hand execution equals right hand execution Enlarged though compact housing and increased shaft height Variable centre distancesImproved component ■YOUR BENEFIT Proven and cost- effective solutionsEasy and fast customizationpossibilitiesIncreased flexibility in mount-ing arrangements Easy change of shaft handing,and only one spare unit need-ed for both The input shaft height matches the large motor shaft height even betterEasy replacement andimprovement of existing instal-lations at a minimum effort and costShorter delivery times POWER & RELIABILITY■FEATURERigid, standardized pinionshafts and housingsFull-scale application of:High torque geometry in low speed gear sets Low noise geometry in high speed gear sets Profile-controlled gas carburizingPrecision grinding with sophisticated tooth contact analysis Assured oil circulation through the bearingsOptimal balance betweenmechanical, thermal and bearing ratingSpecific macro gear geometry■YOUR BENEFITBetter lateral load distribu-tion, lower noise and vibration levelsBetter strength/surfacedurability rating balance Lower noise and vibration levels Optimal load CapacityOptimal transverse and later-al load distribution, lower noise and vibration levels Long, troublefree working life Size is equivalent to power, re-sulting in optimal performance and efficiency for all ratios Reduced power losses Lower noise & vibration levelsReduced operating costs P o w e r a t 150■FEATURE Simple, standard systems for cooling and lubrication Accessories and instrumentation are offered as catalogue options Two mounting positions and alternative mounting with both shafts in a vertical plane Left hand execution equals right hand execution ■YOUR BENEFIT Proven and cost- effective solutions Easy and fast customization possibilities Increased flexibility in mount-ing arrangements Easy change of shaft handing, and only one spare unit need-POWER & RELIABILITY ■FEATURE Rigid, standardized pinion shafts and housings Full-scale application of: High torque geometry in low speed gear sets Low noise geometry in high speed gear sets Profile-controlled gas carburizing Precision grinding with sophisticated tooth contact ■YOUR BENEFIT Better lateral load distribu-tion, lower noise and vibration levels Better strength/surface durability rating balance Lower noise and vibration levels Optimal load Capacity Optimal transverse and later-al load distribution, lowerAccessories and instrumentation ■offered in configured to orderenvironmentRe-use of Hansen P4 gearing■Integrated fan also on motor■lanternEasy and application oriented■State-of-the-art gears with■proven reliabilityExtra thermal capacity■Low noise geometry in high■speed gear setsProfile-controlled gas■carburisingPrecision grinding with high■tech tooth contact analysisHeavy duty bearing concept■Lower noise and vibration■levelsOptimal load capacity■Optimal transverse and lateral■load distribution, lower noiseand vibration levelsHigh external load capacity■INSTALLATIONThe Hansen M-Series models are easy to transport, handle and install.SPARE PARTS, SERVICE & MAINTENANCEHansen M-Series gear units are easy to maintain.NVP3 - drietraps tandwielkast, parallele assen, vertikale LDANVR2 - tweetraps tandwielkast, haakse assen, vertikale LDANVR3 - drietraps tandwielkast, haakse assen, vertikale LDANHP3 - drietraps tandwielkast, parallele assen, hoNHR2 - tweetraps tandwielkast, haakse assen, hNHR3 - drietraps tandwielkast, haakse assen, ha p p l i c a t i o n f o c u s e dScrew pumps …■COOLING TOWERSBrochure: Ref. 603Catalogue: Ref. 604■PULP & PAPERChippers Calenders Winders Brochure: Ref. 609Pumps …Catalogue: Ref. 233Detailed information: refer to factory■AIR COOLED CONDENSERSBrochure: Ref. 603Catalogue: Ref. 604Wet cooling tower drives■WATER TREATMENTCommon aerators Carrousel aerators Brochure: Ref. 605Catalogue: Ref. 606Brush aerators ■MATERIAL HANDLINGConveyors Bucket elevators Travel drives ...Brochure: Ref. 612Catalogue: Ref. 615■MIXERS & AGITATORSChemical industry Petro-chemical Food & drinks ...Brochure: Ref. 608Catalogue: Ref. 607■PEOPLE TRANSPORTBrochure: Ref. 619Dry cooling tower drivesa c o m p l e t e p r o d_gd r i ve p a c k a g e■HORIZONTAL CONFIGURATION• Standard gear units with increased bearingspan or a variety of optional bearing/outputshaft arrangements to absorb a wide range• Designs with hollow output shaft arrange-ments, V-belt connections between themotor and the input shaft of the gear unitand variable speed drives, either electronic y o u r a p p l i c a t i o nFor professional supportHansen Sales and Engineering Centres are located on every continent. The international standards implemented at these centres are your assurance of consistent engineering expertise - a key asset in international projects.For technical supportHansen Transmissions experienced service engi-neers are highly qualified to evaluate problems and recommend the best solutions. Round the clock production requires round the clock sup-port. Hansen Transmissions service engineers are on standby 24 hours a day at Hansen Service Centres around the world - 7 days a week, 365 days a year!For expert trainingHansen Transmissions can conduct customer service training programmes at its Service Centres or at a customer’s own site if requested.For total customer careMore and more customers are looking for total drive solutions from suppliers. HansenTransmissions is exceptionally placed to offer this capability.Close partnerships with several world-class suppliers for motors, couplings, brakes, instru-mentation, bearings, pumps, etc. enables us to offer complete drive solutions.In-house expertise allows to compose the right drive package for your application.Tel.: +32(0)3 450 12 34Fax: +32(0)3 450 15 99 Hansen ServicesHansen Transmissions International N.V.Industrielaan 38-40B-2900 Schoten (Antwerp) Belgium w ww.hansentransmissions.com/servicesservices@hansentransmissions.comWECARE。
ACCA F5考前Tips:考试重点题型解析(上)

ACCA F5考前Tips:考试重点题型解析(上)读完这个东西只需要30分钟,但是做完这个东西拟定的任务需要7天。
但是,正好来得及~拿去!一包干到发燥的,点火就着的干货!Part oneGeneral causes of failure in F5 Exam做题顺序从考后的问询调查来看,相当一批学生一开始就选择攻克后面的大题: 手忙脚乱的先做完大题,发现只有20分钟来做前面的选择题了,因此闭上眼睛勾选,这基本等于自寻死路:不管难易,分值一样。
考试是现实而残酷的游戏,只会以成败论英雄。
要和我煮酒论英雄?我们考完试先!➤Tip: 请先完成SectionA, Section B的选择题, 这是性价比最高的两个部分.时间管理这个是通病,很多同学花时间在他们会做的题目上,跳过不会做的题目...另一位同学的留言: 通宵背了Advanced variance的公式,发现在选择题中用上了,兴奋得两眼放光,一个饿虎扑食,以难分难舍之势跟这个题浪了6分钟!交卷之前不放心,又花了3~4分钟重新验算的一遍!!哥!你对我是真爱,但是我只值2分!本来3.6分钟的事情你花了8分钟!你效率真高!➤Tip: 考前必须最少做10套真题,考前两天最少再做2套历史真题(严格控制时间)生僻考点本次出现了ROLLINGBUDGET的大题. 这个知识点属于常规低频考点(即:授课必讲,但出现在大题的概率不高, 最近一次是2012年12月的Designit Co). 虽然比较生僻,但是知识点覆盖极其常规,一定是Definition(What),Suitability (how), Advantage and Disadvantage (why).按照这个吃遍天下的套路,哪怕只是有模糊印象, 如果可以将这些印象和案例内容结合论述一下, 估计会有30%~40%的有效分。
但是我们一看到这种长得比较陌生的题,第一秒想到的居然不是知识,而是放弃!于是完全不下笔的童鞋很多!你不抓分,分不会从天上掉下来。
美国文学复习资料

American Literature Lecture One 060511/2, 9th Nov. 2009Part I. IntroductionPart I: introduction questions1.Teaching schemes, examination, requirements, advice, contacts, and so on2.What is literature?3.How to define American Literature?4.How to study literature?1. What is literature?1)The definition of 14th century:It means polite learning through reading. A man of literature or a man of letters = a man of wide reading, “literacy”2)The definition of 18th century:practice and profession of writing3)The definition of 19th century:the high skills of writing in the special context of high imagination4)Robert Frost’s definition:performance in words5)Modern definition:We can define literature as language artistically used to achieve identifiable literary qualities and to convey meaningful messages. Literature is characterized by beauty of expression and form and by universality intellectual and emotional appeal.2. How to define the American literatureAmerican literature mainly refers to literature produced in American English by the people living in the United States.3. How to study literatureHistorical Perspectives: Biographical-Historical and Moral-Philosophical.(Diverse Types of Historicisms: including Feminist, Sociological or Marxian Studies of Language, Literature and Translation)Structuralist Perspectives: Looking for Systematic Deep Structures both in Form and Content.(Semiotics, TG Grammar, Systematic/Functional Grammar, Narratology, Freudian psycho-analysis, Russian Formalism, Anglo-American New Criticism, Archetypalism, Myth Criticism, Structural Marxism, Ideology)Poststructuralist or Postmodern Perspectives: Deconstructing Structuring Binaries (No Clear Distinction between Form and Content)[Postmodern Feminism, Postcolonialism, Postmodern Narratologies, New Historicism, Ideological Studies, Discourse Analysis, Reception Theories, Trauma Studies, Trans-Atlantic Studies, Transnationalism, Eco-criticism, Cultural Pathology, and other Postmodernisms]Approaches on Literature1. The Traditional Approaches:1)Analytical ApproachBe familiar with the elements of a literary work, eg: plot, character, setting, point of view, structure, style, atmosphere, theme, etc; answer some basic questions about the text itself.2)Thematic Approach“What is the story, the poem, the play or the essay about?”3)Historical - Biographical Approach4)Moral - Philosophical Approach.2.The Formalistic AppoachStructuralism, Poststructuralism, Semiotics3.The Psychological Approach: Freud4.Mythological and Archetypal Approach5.Feminist Approaches6.Sociological Approach7.Deconstruction8.Phenomenology, Hermeneutics, Reception Theory9.Cultural CriticismAmerican MulticultualismThe New Historicism, British Cultural Materialism10.Additional Approaches:①Aristotlian Criticism②Genre Criticism③Rhetoric, Linguistics, and Stylistics④The Marxist Approach⑤Ecological Criticism⑥Post ColonialismLecture Two 060511/2, 10th Nov. 2009Part II. The periods of American literature①The colonial period (约1607 - 1765)②The period of Enlightenment and the Independence War (1765 -1800)③The romantic period (1800 - 1865)④The realistic period (1865 - 1914)⑤The period of modernism (1914 - 1945)⑥The Contemporary Literature (1945 - 2000)1.The colonial period (约1607 - 1765)The main featuresPuritanism2.The period of Enlightenment and the Independence War (1765 -1800)Benjamin Franklin3.The romantic period (1800 - 1865)1)The early romanticismWashington Irving, James Fenimore Cooper2)“New England Transcendentalism” or “American Renaissance (1836 - 1855)”Emerson, Thoreau/ Whitman, Dickinson/ Hawthorne, Melville , Allan Poe3)“New England Poets” or “Schoolroom Poets”Bryant/ Longfellow/ Lowell/ Holmes/ Whittier4) The Reformers and AbolitionistsBeecher Stowe/ Frederick Douglass4.The realistic period (1865 - 1914)1)Midwestern RealismWilliam Dean Howells2)Cosmopolitan NovelistHenry James3)Local ColorismMark Twain4)NaturalismStephen Crane/ Jack London/ Theodore Dreiser5)The “Chicago School” of PoetryMasters/ Sandburg/ Lindsay/ Robinson6)The Rise of Black American LiteratureWashington/ Du Bois/ Chestnutt5.The period of modernism (1914 - 1945)1)Modern poetry: experiments in form (Imagism)Ezra Pound/ T.S.Eliot/ Robert Frost/ Wallace Stevens/ Carlos Williams2)Prose Writing: modern realism (the Lost Generation)F.Scott Fitzgerald/ Ernest Hemingway/ William Faulkner3)Novels of Social AwarenessSinclair Lewis/ Dos Passos/ John Steinbeck/ Richard Wright4)The Harlem RenaissanceLangston Hughes/ Zora Neals Hurston5)The Fugitives and New Criticism6)The 20th Century American DramaEugene O’ Neil6.The Contemporary Literature (1945 - 2000)I.American Poetry Since 1945: the Anti-traditionII.American Prose Since 1945: Realism and Experimentation.I. Poetry:1)Traditionalism2)Idiosyncratic poets3)Experimental poetry4)Surrealism and Existentialism5)Women and Multiethnic poets6)Chicano / Hispanic / Latino poetry7)Native American poetry8)African-American poetry9)Asian-American poetry10)New DirectionsExperimental Poetry:1)The Black Mountain School2)The San Francisco School3)Beat Poets4)The New York SchoolII. Prose:1.The Realist Legacy and the Late 1940s2.The Affluent but Alienated 1950s3.The Turbulent but Creative 1960s4.The 1970s and 1980s: New Directions1.The Realist Legacy and the Late 1940s1)Robert Penn Warren2)Arthur Miller3)Tennessee Williams4)Katherine Anne Porter5)Eudora Welty2.The Affluent But Alienated 1950s1)John O’Hara2) James Baldwin3) Ralph Waldo Ellison4) Flannery O’Conner5) Saul Bellow6) Bernard Malamud7) Isaac Bashevis Singer8) Vladimir Nabokov9) John Cheever10) John Updike11) J.D.Salinger12) Jack Kerouac3. The Turbulent but Creative 1960s1) Thomas Pynchon2) John Barth3) Norman Mailer4. The 1970s and 1980s: New Directions1) John Gardner2) Toni Morrison3) Alice WalkerPart II. Early American and Colonial Period to 17651. Introduction1. Instead of beginning with folk tales and songs the American literature began with abstractions and proceededfrom philosophy to fiction because there were no written literature among the more than 500 different Indian languages and tribal cultures that existed in North America before the first Europeans arrived there and set up the first colony Jamestown in about 1607.2. American writing began with the work of English adventurers and colonists in the New World chiefly for thebenefit of readers in the mother country. Some of these early works reached the level of literature, as in the robust and perhaps truthful account of his adventures by Captain John Smith and the sober, tendentious journalistic histories of John Winthrop and William Bradford in New England. From the beginning, however, the literature of New England was also directed to the edification and instruction of the colonists themselves, intended to direct them in the ways of the godly.3. Therefore the writing in this period was essentially two kinds: (1) practical matter-of-fact accounts of farming,hunting, travel, etc. designed to inform people “at home” what life was like in the new world, and, often, to induce their immigration; (2) highly theoretical, generally polemical, discussions of religious questions.4. Furthermore, the influential Protestant work ethic, reinforced by the practical necessities of a hard pioneer life,inhibited the development of any reading matter designed simply for leisure-time entertainment.It is the belief that work itself is good in addition to what it achieves; that time saved by efficiency or goodfortune should not be spent in leisure but in doing further work; that idleness is always immoral and likely to lead to even worse sin since “the devil finds work for idle hands to do”. This belief late r developed into the American philosophic idea Puritanism.5. divines who wrote furiously to set forth their views was to defend and promote visions of the religious state. They set forth their visions —in effect the first formulation of the concept of national destiny —in a series ofimpassioned histories and jeremiads from Providence (1654) to Cotton Mather ’s epic Magnalia Christi Americana6. Even Puritan poetry was offered uniformly to the service of God. Michael Wigglesworth ’s Day of Doom (1662) wasuncompromisingly theological, and Anne Bradstreet ’s poems, issued as The Tenth Muse Lately Sprung Up in America (1650), were reflective of her own piety. The best of the Puritan poets, Edward Taylor , whose work was not published until two centuries after his death, wrote metaphysical verse,Sermons and tracts poured forth until austere Calvinism found its last utterance in the words of Jonathan Edwards . In the other colonies writing was usually more mundane and on the whole less notable, though the journal of the Quaker John Woolman is highly esteemed, and some critics maintain that the best writing of the colonial period is found in the witty and urbane observations of William Byrd , a gentleman planter of Westover, Virginia.2. The Main Features of this period1) American literature grew out of humble origins. diaries, histories, journals, letters, commonplace books, travelbooks, sermons, in short, personal literature in its various forms, occupy a major position in the literature of the early colonial period.2) In content these early writings served either God or colonial expansion or both. In form, if there was any format all, English literary traditions were faithfully imitated and transplanted.3) The Puritanism formed in this period was one of the most enduring shaping influences in American thought andAmerican literature.3. Puritanism1) Simply speaking, American Puritanism just refers to the spirit and ideal of puritans who settled in the NorthAmerican continent in the early part of the seventeenth century because of religious persecutions. In content it means scrupulous moral rigor, especially hostility to social pleasures and indulgences, that is strictness,sternness and austerity in conduct and religion.2) With time passing it became a dominant factor in American life, one of the most enduring shaping influences inAmerican thought and American Literature. To some extent it is a state of mind, a part of the national cultural atmosphere that the American breathes, rather than a set of tenets.3) Actually it is a code of values, a philosophy of life and a point of view in American minds, also a two-facetedtradition of religious idealism and level-headed common sense.Part III. The period of Enlightenment and the Independence War (1765 -1800)I. Introduction1) The 18th-century American enlightenment as a movement marked by an emphasis on rationality rather thantradition, scientific inquiry instead of unquestioning religious dogma, and representative government in place of monarchy.2) Enlightenment thinkers and writers, such as Benjamin Franklin and Thomas Paine, were devoted to the idealsof justice, liberty, and equality as the natural rights of man.3) In these period with the exception of outstanding political writing, such as Common sense, Declaration ofIndependence, The Federalist Papers and so on, few works of note appeared. Even if there appeared poetry and fiction, they were full of imitativeness and vague universality. So most Americans were painfully aware of their excessive dependence on English literary models. The search for a native literature became a national obsession.4) Despite these we should pay attention to several points in this period:William Hill Brown (1765-1793) published the first American novel The Power of Sympathy in 1789.Charles Brockden Brown (1771-1810) was the first American author to attempt to live from his writing. Hedeveloped the genre of American Gothic.The Dictionary edited by Noah Webster (1758-1843) based the American lexicography. Updated Webster’sdictionaries are still standard today.Philip Freneau’s (1752-1832) was known as "the poet of the American Revolution". His major themes are death, nature, transition, and the human in nature. All of these themes become important in 19th century writing. All the while...in romanticizing the wonders of nature in his writings...he searched for an American idiom in verse. II. Benjamin Franklin1706 - 1790(An Extraordinary Life and An Electric Mind)1. His Life1)Born the tenth of fifteen children in a poor candle and soap maker’s family, he had to leave school before he waseleven.2)At twelve he was apprenticed to an older brother, James, a printer in Boston.3)As a voracious reader he managed to make up for the deficiency by his own effort and began at 16 to publishessays under the pseudonym, Silence Dogood, essays commenting on social life in Boston.4)When he was 17 he ran away to Philadelphia to make his own fortune marking the beginning of a long successstory of an archetypal kind.5)He set himself up as an independent printer and publisher, found the Junto Club and subscription library,issued the immensely popular Poor Richard’s Almanac.6)Retired around forty-two, he did what was to him a great happiness: read, make scientific experiments and dogood to his fellowmen. He helped to find the Pennsylvania Hospital, an academy which led to the University of Pennsylvania, and the American Philosophical Society.7)At the same time he did a lot of famous experiments and invented many things such as volunteer firedepartments, effective street lighting, the Franklin Stove, bifocal glasses, efficient heating devices, lightning-rod and so on.8)Beginning his public career in the early fifties, he became a member of the Pennsylvania Assembly, the DeputyPostmaster-General for the colonies, and for some eighteen years served as representative of the colonies in London.9)During the War of Independence, he was made a delegate to the Continental Congress and a member of thecommittee to write the Declaration of Independence. One of the makers of the new nation, he was instrumental in bringing France into an alliance with America against England, and played a decisive role at the Constitutional Convention.2. Major Works1)Poor Richard’s AlmanacMaxims(谚语,格言)and axioms(哲理,格言)a)Lost time is never found again.b) A penny saved is a penny earned.c)God help them that help themselves.d)Fish and visitors stink in three days.e)Early to bed, and early to rise, makes a man healthy, wealthy, and wise.f)Ale in, truth out.g)Eat not to dullness. Drink not to elevation.h)Diligence is the Mother of Good Luck.i)One Today is worth two tomorrow.j)Industry pays debts. Despair encreaseth them.2)Autobiographya.It is perhaps the first real post-revolutionary American writing as well as the first real autobiography in English.b.It gives us the simple yet immensely fascinating record of a man rising to wealth and fame from a state ofpoverty and obscu rity into which he was born, the faithful account of the colorful career of America’s first self-made man.c.First of all, it is a puritan document. The most famous section describes his scientific scheme of self-examinationand self-improvement.d.It is also an eloquent elucidation of the fact that Franklin was spokesman for the new order of eighteenthcentury enlightenment, and that he represented in America all its ideas, that man is basically good and free, by nature endowed by God with certain inalienable rights of liberty and the pursuit of happiness.e.It is the pattern of Puritan simplicity, directness, and concision. The plainness of its style, the homeliness ofimagery, the simplicity of diction, syntax and expression are some of the salient features we cannot mistake.3. Evaluation1)He was a rare genius in human history. Nature seemed particularly lavish and happy when he was shaped.Everything seems to meet in this one man, mind and will, talent and art, strength and ease, wit and grace, and he became almost everything: a printer, postmaster, citizen, almanac maker, essayist, scientist, inventor, orator, statesman, philosopher, political economist, ambassador, musician and parlor man.2)He was the first great self-made man in America, a poor democrat born in an aristocratic age that his fineexample helped to liberalize.3)Politically he brought the colonial era to a close. For quite some time he was regarded as the father of allYankees, even more than Washington was. He was the only American to sign the four documents that created the United States: the Declaration of Independence, the treaty of alliance with France, the treaty of peace with England, and the constitution.4)Scientifically, as the symbol of America in the Age of Enlightenment, he invented a lot of useful implements. Hisresearch on electricity, his famous experiment with his kite line and many others made him the preeminent scientist of his day.5)Literally, he really opened the story of American literature. D. H. Lawrance agreed that Franklin waseverything but a poet. In the Scottish philosopher David Hume’s eyes he was America’s “first great man of letters”.Assignment: Please read the material by Ralph Waldo EmersonLecture Three 060511/2, 16th Nov. 2009The American Romanticism(I)I. What is RomanticismSimply speaking, Romanticism is a literary movement flourished as a cultural force throughout the 19th C and it can be divided into the early period and the late period. Also it remains powerful in contemporary literature and art.Romanticism, a term that is associated with imagination and boundlessness, as contrasted with classicism, which is commonly associated with reason and restriction. A romantic attitude may be detected in literature of any period, but as an historical movement it arose in the 18th and 19th centuries, in reaction to more rational literary, philosophic, artistic, religious, and economic standards.... The most clearly defined romantic literary movement in the U. S. was Transcendentalism.The representatives of the early period includes Washington Irving and James Fenimore Cooper, and those of the late period contain Ralph Waldo Emerson, Henry David Thoreau, Walt Whitman, Nathaniel Hawthorne, Herman Melville, Edgar Allan Poe.II. The reasons on the rise of American RomanticismInternal causes:1)American burgeoned into a political, economic and cultural independence. Democracy and political equalitybecame the ideals of the new nation. Radical changes came about in the political life of the country. Parties began to squabble and scramble for power, and new system was in the making.2)The spread of industrialism, the sudden influx of immigration, and the pioneers pushing the frontier furtherwest, all these produced something of an economic boon and, with it, a tremendous sense of optimism and hope among the people.3)Ever-increasing magazines played an important role in facilitating literary expansion in the country.External causes:1)Foreign influences added incentive to the growth of romanticism in America.2)The influence of Sir Walter Scott was particularly powerful and enduring.III. Characteristics of American Romanticism (b)1)Sentimentalism, primitivism and the cult of the noble savage2)Political liberalism3)The celebration of natural beauty and the simple life4)Introspection5)The idealization of the common man, uncorrupted by civilization6)Interest in the picturesque past and remote places7)Antiquarianism8)Individualism9)Morbid melancholy10)Historical romanceIV. The Representatives of the early American romanticismA. Washington Irving(1783-1859 )1. About the Author1)Washington Irving was born in New York City on April 3, 1783 as the youngest of 11 children. His parents,Scottish-English immigrants, were great admirers of General George Washington, and named their son after their hero.2)Early in his life Irving developed a passion for books. He studied law privately but practiced only briefly. From1804 to 1806 he travelled widely in Europe. After returning to the United States, Irving was admitted to the New York bar in 1806.3)He was a partner with his brothers in the family hardware business and representative of the business inEngland until it collapsed in 1818. During the war of 1812 Irving was a military aide to New York Governor Tompkins in the U.S. Army.4)Irving's career as a writer started in journals and newspapers. His success in social life and literature wasshadowed by a personal tragedy because his engaged love died at the age of seventeen. So he never married or had children.5)After the death of his mother, Irving decided to stay in Europe, where he remained for seventeen years from1815 to 1832.6)In 1832 Irving returned to New York to an enthusiastic welcome as the first American author to have achievedinternational fame. Between the years 1842-45 Irving was the U.S. Ambassador to Spain.7)Irving spent the last years of his life in Tarrytown. From 1848 to 1859 he was President of Astor Library, laterNew York Public Library. Irving's later publications include Mahomet And His Successors(1850), Wolfert's Roost(1855), and his five-volume The Life of George Washington(1855-59). Irving died in Tarrytown on November 28, 1859.2. His Major Works1)His earliest work was a sparkling, satirical History of New York (1809) under the Dutch, ostensibly written byDiedrich Knickbo cker (hence the name of Irving’s friends and New York writers of the day, the “Knickbocker School”.)2)The Sketch Book (1819-20 as Geoffrey Crayon) - contains 'Rip Van Winkle' and 'The Legend of Sleepy Hollow'3)The Life of George Washington (1855-59, five volumes)3. Evaluation to him1)American author, short story writer, essayist, poet, travel book writer, biographer, and columnist. Irving hasbeen called the father of the American short story. He is best known for 'The Legend of Sleepy Hollow,' in which the schoolmaster Ichabold Crane meets with a headless horseman, and 'Rip V an Winkle,' about a man who falls asleep for 20 years.2)The first American writer of imaginative literature to gain international fame, so he was regarded as father ofAmerican literature.3)The short story as a genre in American literature probably began with Irving’s The Sketch Book, ACOLLECTION OF ESSAYS, SKETCHES, AND TALES. It also marked the beginning of American Romanticism.B. James Fenimore Cooper (1789-1851)1. His Major WorksIn his life Cooper wrote over thirty novels which can be divided into frontier novels, detective novels and reference novels. He considered The Pathfinder (1840) and The Deerslayer (1841) his best works.The unifying thread of the five novels collectively known as the Leather-Stocking Tales is the life of Natty Bumppo. Cooper’s finest achievement, they constitute4 a vast prose epic with the North American continent as setting. Indian tribes as Characters, and great wars and westward migration as social background. The novels bring to life frontier America from 1740 to 1804.1)The Pioneers(1823): Natty Bumppo first appears as a seasoned scout in advancing years, with the dyingChingachgook, the old Indian chief and his faithful comrade, as the eastern forest frontier begins to disappear and Chingachgook dies.2)The Last of the Mohicans(1826): An adventure of the French and Indian Wars in the Lake George county.3)The Prairie(1827): Set in the new frontier where the Leatherstocking dies.4)The Pathfinder(1840): Continuing the same border warfare in the St. Lawrence and Lake Ontario county.5)The Deerslayer(1841): Early adventures with the hostile Hurons on Lake Otsego, NY.2. Contributions of CooperThe creation of the famous Leatherstocking saga has cemented his position as our first great national novelist and his influence pervades American literature. In his thirty-two years (1820-1851) of authorship, Cooper produced twenty-nine other long works of fiction and fifteen books - enough to fill forty-eight volumes in the new definitive edition of his Works. Among his achievements:1)The first successful American historical romance in the vein of Sir Walter Scott (The Spy, 1821).2)The first sea novel (The Pilot, 1824).3)The first attempt at a fully researched historical novel (Lionel Lincoln, 1825).4)The first full-scale History of the Navy of the United States of America (1839).5)The first American international novel of manners (Homeward Bound and Home as Found, 1838).6)The first trilogy in American fiction (Satanstoe, 1845; The Chainbearer, 1845; and The Redskins, 1846).7)The first and only five-volume epic romance to carry its mythic hero - Natty Bumppo - from youth to old age. 3. His Skills1)He is good at making plots.2)All his novels are full of myths.3)He had never been to the frontier and among the Indians and yet could write five huge epic books about them isan eloquent proof of the richness of his imagination.4)He created the first Indians to appear in American fiction and probably the first group of noble savages.5)He hi t upon the native subject of frontier and wilderness, and helped to introduce the “Western” tradition intoAmerican literature.V. American Renaissance1. The Concept1)It also called New England Renaissance period from the 1830s roughly until the end of the American CivilWar in which American literature, in the wake of the Romantic movement, came of age as an expression of a national spirit.2)The literary scene of the period was dominated by a group of New England writers, the “Brahmins”. They werearistocrats, steeped in foreign culture, active as professors at Harvard College, and interested in creating a genteel American literature based on foreign models.3)One of the most important influences in the period was that of the Transcendentalists, including Emerson,Thoreau and so on.4)The Transcendentalists contributed to the founding of a new national culture based on native elements. Theyadvocated reforms in church, state, and society, contributing to the rise of free religion and the abolition movement and to the formation of various utopian communities, such as Brook Farm. The abolition movement was also bolstered by other New England writers, including the Quaker poet Whittier and the novelist Harriet Beecher Stowe, whose Uncle Tom's Cabin (1852) dramatized the plight of the black slave.5)Apart from the Transcendentalists, there emerged during this period great imaginative writers—NathanielHawthorne, Herman Melville, and Walt Whitman—whose novels and poetry left a permanent imprint on American literature. Contemporary with these writers but outside the New England circle was the Southern genius Edgar Allan Poe, who later in the century had a strong impact on European literature.Lecture Four The American Romanticism(II)TranscendentalismIt is a 19th-century movement of writers and philosophers in New England who were loosely bound together by adherence to an idealistic system of thought.Emerson defined it as “idealism” simply. In reality it was far more complex collection of beliefs: that the spark of divinity lies within man; that everything in the world is a microcosm of existence; that the individual soul is identical to the world soul, or Over-Soul. By meditation, by communing with nature, through work and art, man could transcend his senses and attain an understanding of beauty and goodness and truth.In application, American transcendentalism urged a reform in society, and that such a reform may be reached if individuals resist customs and social codes, and rely rather on reason to learn what is right. Ultimately, transcendentalists believed that one should transcend society's code of ethics and rely on personal intuition in order to reach absolute goodness, or Absolute Truth.It was indebted to the dual heritage of American Puritanism. That is to say, it was in actuality romanticism on the puritan soil.Transcendentalism dominated the thinking of the American Renaissance, and its resonance reverberated through American life well into the 20th century. In one way or another American most creative minds were drawn into its thrall, attracted not only to its practicable messages of confident self-identity, spiritual progress and social justice, but also by its aesthetics, which celebrated, in landscape and mindscape, the immense grandeur of the American soul.The Representativesof American RenaissanceI. The Essayists1)Ralph Waldo Emerson2)Henry David ThoreauRalph Waldo Emerson(1803 - 1882)1.His philosophy:1)Strongly he felt the need for a new national vision.2)He firmly believes in the transcendence of the Oversoul and thought that the universe was composed of Nature。
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Abstract It has been hypothesized that Tree Adjoining Grammar (TAG) is particularly well suited for sentence generation. It is unclear, however, how a sentence generation system based on TAG should choose among the syntactic possibilities made available in the grammar. In this paper we consider the question of what needs to be done to generate with TAGs and explain a generation system that provides the necessary features. This approach is compared with other TAG-based generation systems. Particular attention is given to Mumble-86 which, like our system, makes syntactic choice on sophisticated functional groification). An L-Spec captures the content of what is to be generated along with the goals and rhetorical force to be achieved. While the form of the L-Spec is dependent on the particular application, for the purposes of this discussion we can think of it as a set of logical form expressions that describe the content to be expressed. Mumble-86 uses a dictionary-like mechanism to transform a piece of the L-Spec into an elementary TAG tree which realizes that piece. The translation process itself (performed in the dictionary) may be influenced by contextual factors (including pragmatic factors which are recorded as a side-effect of g r a m m a r routines), and by the goals recorded in the L-Spec itself. It is in this way that the system can make fine-grained decisions concerning one realization over another. Once a TAG tree is chosen to realize the initial subpiece, that structure is traversed in a left to right fashion. G r a m m a r routines are run during this traversal to ensure grammaticality (e.g., subject-verb agreement) and to record contextual information to be used in the translation of the remaining pieces of the L-Spec. In addition to the g r a m m a r routines, as the initial tree is traversed at each place where new information could be added into the evolving surface structure (called attachment points), the remaining L-Spec is consulted to see if it contains an item whose realization could be adjoined or substituted at that position. In order for this methodology to work, (McDonald & Pustejovsky, 1985) point out that they have to make some strong assumptions about the logical form input to their generator. Notice that the methodology described always starts generating from an initial tree and other auxiliary or initial trees are adjoined or substituted into that initial structure. 3 As a result, in generating an embedded sentence, the generator must start with the innermost clause in order to ensure that the first tree chosen is an initial (and not an auxiliary) tree. Consider, for example, the generation of the sentence "Who did you think hit John". Mumble86 must start generating from the clause "Who hit John" which is (roughly) captured in the tree shown in Figure 4. This surface structure would then be traversed. At the point labeled fr-node (an attachment point) the auxiliary tree representing "you think" in Figure 2 would be adjoined in. Notice, however, that if Mumble-86 must work from the inner-most clause out, then the initial L-Spec must be in a particular form which is not consistent with the "logician's usual represen3An initial tree is a minimal non-recursive structure in TAG, wlfile an auxiliary tree is a minimal recursive structure. Thus, an auxiliary tree is characterized as having a leaf node (wlfich is termed the foot node) which has the s a m e label as the root node. T h e tree in Figure 2 is an auxiliary tree. The adjoining operation essentially inserts an auxiliary tree into another tree. For instance, the tree in Figure 5 is the result of adjoining the auxiliary tree shown in Figure 2 into the ilfitial tree shown in Figure 4 at the n o d e labeled It-node.
A Functional Approach to Generation
with TAG 1
K a t h l e e n F. M c C o y , K. V i j a y - S h a n k e r , & G i j o o Y a n g D e p a r t m e n t of C o m p u t e r a n d I n f o r m a t i o n Sciences U n i v e r s i t y of D e l a w a r e N e w a r k , D e l a w a r e 19716, U S A email: mccoy@, vijay@
1 Introduction
Joshi (1987) described the relevance of Tree Adjoining G r a m m a r (TAG) (Joshi, 1985; Schabes, Abeille &5Joshi, 1988) to Natural Language Generation. In particular, he pointed out how the unique factoring of recursion and dependencies provided by TAG made it particularly appropriate to derive sentence structures from an input provided by a text planning component. Of particular importance is the fact that (all) syntactic dependencies and function argument structure are localizest in TAG trees. Shieber and Schabes (1991) discuss using Synchronous TAG for generation. Synchronous TAG provides a formal foundation to make explicit the relationship between elementary syntactic structures and their corresponding semantic counterparts, both expressed as elementary TAG trees. This relationship is made explicit by pairing the elementary trees in the syntactic and logical form languages, and associating the corresponding nodes. Shieber and Schabes (1990) describe a generation algorithm which "parses" an input logical form string recording the adjoining and substitution operations necessary to build the string from its elementary components. The corresponding syntactic structure is then generated by doing 1This work is supported ill part by Grant #H133E80015 from the National hlstitute on Disability and Rehabilitation Research. Support was also provided by the Nemours Fotmdation. We would like to thank John Hughes for Iris many conunents and discussions concerning this work.