SNT 1196-2003 玉米中转基因成分定性PCR检测方法

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NPTIIl neomycin-3'-phosphotransferase genet%#* - 3 ' - @ @ 8 @ & H . NOS1 terminator of nopaline synthase gene,861813U&9ElSm&&k+. IVS21intron from the maize alcohol dehydrogenase gene,3#B)rB#%#%ElW&BJfi E 2. PATt phosphinothricin acetyltranrferase gene*PTf#Z,B%##Sfl. HSP~O;O. Kb intron from the hsp70 gene (heat-shock protein) ,A Q%#j&@Q(hsp70)S 8 $1BO 0.8KbLBk4LX. 3. 3 - 2 2 CryIA(b) 1 a synthetic gene encodes the first 648 amino a~ids~insecticidal-active ftruncated product identical to that of CrylA(b) gene of Bacillus thuringisnsi, s u b s p , % Z & ~ ~ f i ~ ~ & e ~ crylA(b)Sa. 3.3.23 CDPK I calcium-dependent protein kinase(CDPK) gene, $5%$&% & #@S$1. 3.3. 24 Cry9CI insecticidal-active ftruncated product identical to that of C r g C gene of Bacillus ~ C sm. thuringiensis s u b s p , ~ Z & i $ ~ f i ~ & & ~C r lg& l 3.3.25 CaMV35SI 135sterminator of CaMV g e n o m c r t E ~ # i % R i - ~ ~ S35sl%kF. H ~ 3.3.26 P actin 1I the promoter derived from the rice (Oryza sativa) actin 1 g e n e , A % F h ) / t ~ $ j ~
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