测序通用引物列表

测序通用引物列表

测序通用引物列表

载体名称上游引物（5'primer）下游引物（3'primer）

P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152) pAc5.1/V5-His(_A,_B,_C) pAc5-5 BGH rev pAcG2T pGEX-5 not available

pACT T7EEV T3/EBVrev

pACT2 GAL4 ADfor pGAL4-ADrv

pACT2 p17110 p12584

pACYC184(BamHI-site) PBRforBam PBRrevBam

pACYCDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1

pACYCDuet-1(MCSII) DuetUp2 T7-Terminator pAdEasy-1 not available not available

pAdTrack-CMV not available not available pAS2-1 GAL4 Bdfor/P24990 pGAL4-BDrv/P31430

pB42AD pB42ADF PB42ADR

pBABE pBMN_5'

pBacPAK8 BAC1 BAC2

pBAD pBAD-F pBAD-R pBAD/HisABC pBAD-5'

pBBR1MCS T3/M13rev T7/M13for

pBD-GAL4 pBD-GAL4-F PBD-GAL4-R

pBI121 35S NOS

pBIND GAL4-Bdfor T3,EBVrev

pBK-CMV M13F/T7 M13R/T3 pBluescriptIIKS(+/-) T3/M13rev T7/M13for（优先用M13F）pBluescriptIISK(+/-) T3/M13rev T7/M13for

pBluescriptKS(+/-) T3/M13rev T7/M13for

pBluescriptSK(+/-) T3/M13rev T7/M13for

pBR322(BamHI-site) PBRforBam PBRrevBam

pBR322(EcoRI/HindIII-sites) pBRforEco pBRrevHind pBR325(BamHI-site) PBRforBam PBRrevBam

pBR329(BamHI-site) PBRforBam PBRrevBam pBridge(MCSI) GAL4-Bdfor 3'BD

pBridge(MCSII) not available not available pBS-T II T3/M13rev T7/M13for pBS（AMP）T7 T3 pBSR T3 T7ter pBudCE4.1(MCSI) CMV-Profor,T7 c-mycrev,EBVrev

pBudCE4.1(MCSII) EF-1α Fwd BGH

pBudCE4.1/lacZ/CAT CMV-Profor,T7 c-mycrev,EBVrev pBV220 pBV220F PBV220R

pCAL-c T7/Tac-Profor T7-Terminator

pCAL-n T7/Tac-Profor T7-Terminator pCAMBIA1301(1300) pCAMBIA1301-5'(ECORI)/M13R pCAMBIA1301-3'(HindIII) pCAMBIA1304 pCAMBIA1301-5'(ECORI) pCAMBIA1301-3'(HindIII) pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49) pCANTB5E S1 S6

pCAT3-enhancer RVP3 此载体无反向引物pCDFDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1

pCDFDuet-1(MCSII) DuetUP2 T7TER pcDNA1.1 CMV-Profor,T7 pCDM8-3

pcDNA2.1 M13for/T7 M13rev/Tac-Profor

pcDNA3 T7/CMV-Profor BGH rev/SP6 pcDNA3.1(+/-) pcDNA3.1F/T7/CMV-Profor BGH rev pcDNA3.1/His(_A,_B,_C) T7/CMV-Profor BGH rev pcDNA3.1/myc-His(_A,_B,_C) T7/CMV-Profor BGH rev/c-mycrev pcDNA3.1/V5-His(_A,_B,_C) T7/CMV-Profor BGH rev/V5rev pcDNA3.1/Hygro(+,-) T7/CMV-Profor BGH rev/V5rev

pcDNA3.1/Zeo(+.-) T7 BGHrev

pcDNA3.3TOPOTA CMV-Profor TKPAreverse pcDNA4/HisMax(_A,_B,_C) Xpressfor BGH rev

pcDNA4/HisMax-TOPO Xpressfor BGH rev pcDNA4/HisMax-TOPO/lacZ Xpressfor BGH rev pcDNA4/myc-His(_A,_B,_C) T7,CMV-Profor BGH rev,c-mycrev pcDNA4/TO TREfor/CMV-Profor BGH rev pcDNA4/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pcDNA4-TET/ON/AMP+ CMV-Profor

pcDNA5/FRT T7/CMV-Profor BGH rev pcDNA6/myc-His(_A,_B,_C) CMV-Profor,T7 BGH rev,c-mycrev pcDNA6/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pCEP4 pCEP-F/CMV-Profor EBVrev

pCI T7（17BASE）EBVrev

pCI-neo T7（17BASE）EBVrev,T3

pCMS-EGFP T7 T3

pCMS-EGFP T7 EBVrev,T3

pCMV-3Tag-4A T3 /PFLAG-CMV-F T7 pCMV5 pCMV5F PCMV5R

pCMV6 CMV-Profor pCMV6-3、HGHrev

pCMV6-XL4 CMV-Profor/T7 pCMV6-3、HGHrev

pCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrev pCMV-MYC(AMP+) PCMV-F PCMV-R, EBVrev

pCMVmyc/nuc CMV-Profor BGH rev,c-mycrev pcmv-scriptEXVector T3 T7 pCMV-Sport6 T7/M13for SP6/M13rev pCOLADuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1 pCOLADuet-1(MCSII) DuetUP2 T7TER pCR2.1 T7,M13for M13rev pCR2.1-TOPO M13for,T7 M13rev pCR3.1 T7 BGH rev pCR3.1-Uni T7 BGH rev pCR4 T3/M13rev T7/M13for pCR4-Blunt T3/M13rev T7/M13for

pCR4-TOPO M13rev,T3 M13for,T7

pCR-Blunt M13rev T7,M13for

pCR-BluntII M13rev/SP6 T7/M13for pCR-BluntII-TOPO M13rev/SP6 M13for/T7 pCRII M13rev/SP6 T7/M13for pCRII-