酵母双杂交原理ppt演示

一. 酵母双杂交系统原理
(Yeast Two-hybrid System)
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Yeast twBiblioteka Baidu-hybrid system:
a genetic assay for detecting protein-protein interactions
Suppose we have two proteins...
X
Y
and the question, do these two protein bind each other?
One way of answering this question is to use the yeast two hybrid method.
To understand the method we must first consider how gene expression is regulated in yeast......
Yeast two-hybrid system:
a genetic assay for detecting protein-protein interactions Regulation of gene expression in yeast
ti a rtt a c ni s v c a r pr i o n t o
ti a rtt a c ni s v c a r pr i o n t o
ao c m tn i d va ai t i o n
Dd No A a b i ig n n d i m n
U A S (t a n c u c s e p ti e ) s i oe r ev q aa u m t n
ti m rt c a a n h s i c n r pe i o n r y
rr e e g p n o e t e r
His, β-gal
Yeast two-hybrid system:
a genetic assay for detecting protein-protein interactions Regulation of gene expression in yeast
ge e n
and now back to the question...
Yeast two-hybrid system:
a genetic assay for detecting protein-protein interactions Regulation of gene expression in yeast
ge e n
D th s o ee p te s ro in b d in ?
Y
X
X
X Y
ot o b u hr r w i yd pe b ! r isn on id t
Y
t f i at a h m cl u nn s g i o f o r u n t sov. X r c na apc r n i at r i t to i . .
ti a rtt a c ni s v c a r pr i o n t o
ao c m tn i d va ai t i o n
Dd No A a b i ig n n d i m n
U A S (t a n c u c s e p ti e ) s i oe r ev q aa u m t n
ti m rt c a a n h s i c n r pe i o n r y
ao c m tn i d va ai t i o n
Dd No A a b i ig n n d i m n
U A S (t a n c u c s e p ti e ) s i oe r ev q aa u m t n
ti m rt c a a n h s i c n r pe i o n r y
ge e n
D t ee o hs t opoen w rti bn ? i d
Y
X
We start with yeast possessing a reporter gene (a gene making a product that is easy to detect).
And now we introduce genes coding for two hybrid proteins.....
yes, we have expression of the reporter indicating that the proteins bind!
Y
do we get expression of the reporter?
rr e e g p n o e t e r
His, β-gal