越桔提取物花青素

越桔提取物中花青素（Anthocyanidin）含量测定方法

一、仪器与试剂

紫外—可见光分光光度计.

无水乙醇（A.R.）

37%浓盐酸

5%盐酸甲醇溶液：取5ml 37%浓酸盐加甲醇至100 ml。

二、样品溶液制备

准确称取越桔提取物约10mg，置100ml 圆底烧瓶中，加入60ml 5%的盐酸-甲醇溶液，沸水浴中加热回流60min，放凉转移至100ml 容量瓶中，用5%的盐酸-甲醇溶液定容，再吸取10ml用5%的盐酸-甲醇溶液稀释至100ml作为供试样品溶液。

用1cm比色皿，以5%的盐酸-甲醇溶液做空白在535nm处测其吸光度（A）。

计算公式如下（以飞燕草素计）：

A×1000

含量（%）＝

1020×W

式中：A——样品吸光度

1020——为飞燕草素的吸收系数

W——样品称样量（g）

花色甙（Anthocyanosides）含量%＝飞燕草素（delphinidin）%×1.44|

Method of determination of anthocyanidin in Bilberry Extract 1、Apparatus and reagent

Ultra – violet visible spectrophotometer

Anhydrous alcohol (A.R)

37% hydrochloric acid

5% hydrochloric acid-methanol solution: 5ml hydrochloric acid was added into methanol to reach 100 ml

2、Preparation of sample solution

Accurately weigh some 10mg Bilberry P.E. into a 100ml Erienmeyer flask, dilute with 60ml 5% hydrochloric acid-methanol solution. The solution was heated and refluxed in boiled water bath for 60mins and transferred to 100ml volumetric flask after its cooling down. 5% hydrochloric acid-methanol solution was used to reach the volume. Extract 10 ml, then use 5% hydrochloric acid-methanol solution to dilute to 100ml, obtain the sample.

1cm cell was used. Use 5% hydrochloric acid-methanol solution as blank at 535nm to determine the absorbance capacity of the sample solution.

3、Calculate the result by formula (calculated by delphinidin)

A×1000

Content (%)＝

1020×W

A: absorbance of sample

1020: absorbance coefficient of delphinidin

W: weight of sample (g)

Anthocyanosides%＝delphinidin%×1.44