pAAV-CaMKIIa-EGFP腺病毒载体说明(图)

pAAV-­‐CaMKIIa-­‐EGFP

编号 载体名称 北京华越洋VECT10001 pAAV-­‐CaMKIIa-­‐EGFP

pAAV-­‐CaMKIIa-­‐EGFP载体基本信息

载体名称: pAAV-­‐CaMKIIa-­‐EGFP

质粒类型: 腺病毒载体；荧光报告载体

高拷贝/低拷贝: 低拷贝

克隆方法: 限制性内切酶，多克隆位点

启动子: CaMKIIa

载体大小: 6092 b p

5' 测序引物及序列: ATGCTGACGAAGGCTCGCGA

3' 测序引物及序列: GCATTAAAGCAGCGTATCCACATAGC

载体标签: -­‐-­‐

载体抗性: 氨苄青霉素

筛选标记: EGFP

克隆菌株: Stbl3 或 NEB S tble

宿主细胞（系）: 神经细胞

备注: -­‐-­‐

稳定性: 稳表达

组成型/诱导型: 组成型

病毒/非病毒: 腺病毒

pAAV-­‐CaMKIIa-­‐EGFP载体质粒图谱和多克隆位点信息

pAAV-­‐CaMKIIa-­‐EGFP载体序列:

ORIGIN

1 C CTGCAGGCA G CTGCGCGCT C GCTCGCTCA C TGAGGCCGC C CGGGCAAAG C CCGGGCGTC 61 G GGCGACCTT T GGTCGCCCG G CCTCAGTGA G CGAGCGAGC G CGCAGAGAG G GAGTGGCCA 121 A CTCCATCAC T AGGGGTTCC T GCGGCCGCA C GCGTTTAAC A TTATGGCCT T AGGTCACTT

181 C ATCTCCATG G GGTTCTTCT T CTGATTTTC T AGAAAATGA G ATGGGGGTG C AGAGAGCTT 241 C CTCAGTGAC C TGCCCAGGG T CACATCAGA A ATGTCAGAG C TAGAACTTG A ACTCAGATT 301 A CTAATCTTA A ATTCCATGC C TTGGGGGCA T GCAAGTACG A TATACAGAA G GAGTGAACT 361 C ATTAGGGCA G ATGACCAAT G AGTTTAGGA A AGAAGAGTC C AGGGCAGGG T ACATCTACA 421 C CACCCGCCC A GCCCTGGGT G AGTCCAGCC A CGTTCACCT C ATTATAGTT G CCTCTCTCC

481 A GTCCTACCT T GACGGGAAG C ACAAGCAGA A ACTGGGACA G GAGCCCCAG G AGACCAAAT 541 C TTCATGGTC C CTCTGGGAG G ATGGGTGGG G AGAGCTGTG G CAGAGGCCT C AGGAGGGGC 601 C CTGCTGCTC A GTGGTGACA G ATAGGGGTG A GAAAGCAGA C AGAGTCATT C CGTCAGCAT 661 T CTGGGTCTG T TTGGTACTT C TTCTCACGC T AAGGTGGCG G TGTGATATG C ACAATGGCT 721 A AAAAGCAGG G AGAGCTGGA A AGAAACAAG G ACAGAGACA G AGGCCAAGT CAACCAGACC

781 A ATTCCCAGA G GAAGCAAAG A AACCATTAC A GAGACTACA A GGGGGAAGG G AAGGAGAGA 841 T GAATTAGCT T CCCCTGTAA A CCTTAGAAC C CAGCTGTTG C CAGGGCAAC G GGGCAATAC 901 C TGTCTCTTC A GAGGAGATG A AGTTGCCAG G GTAACTACA T CCTGTCTTT C TCAAGGACC 961 A TCCCAGAAT G TGGCACCCA C TAGCCGTTA C CATAGCAAC T GCCTCTTTG C CCCACTTAA

1021 T CCCATCCCG T CTGTTAAAA G GGCCCTATA G TTGGAGGTG G GGGAGGTAG G AAGAGCGAT 1081 G ATCACTTGT G GACTAAGTT T GTTCGCATC C CCTTCTCCA A CCCCCTCAG T ACATCACCC

1141 T GGGGGAACA G GGTCCACTT G CTCCTGGGC C CACACAGTC C TGCAGTATT G TGTATATAA 1201 G GCCAGGGCA A AGAGGAGCA G GTTTTAAAG T GAAAGGCAG G CAGGTGTTG GGGAGGCAGT

1261 T ACCGGGGCA A CGGGAACAG G GCGTTTCGG A GGTGGTTGC C ATGGGGACC T GGATGCTGA 1321 C GAAGGCTCG C GAGGCTGTG A GCAGCCACA G TGCCCTGCT C AGAAGCCCC A AGCTCGTCA 1381 G TCAAGCCGG T TCTCCGTTT G CACTCAGGA G CACGGGCAG G CGAGTGGCC C CTAGTTCTG 1441 G GGGCAGCTC T AGAGCGGTA C CGGATCCGC C ACCATGGTG A GCAAGGGCG A GGAGCTGTT 1501 C ACCGGGGTG G TGCCCATCC T GGTCGAGCT G GACGGCGAC G TAAACGGCC A CAAGTTCAG 1561 C GTGTCCGGC G AGGGCGAGG G CGATGCCAC C TACGGCAAG C TGACCCTGA A GTTCATCTG 1621 C ACCACCGGC A AGCTGCCCG T GCCCTGGCC C ACCCTCGTG A CCACCCTGA C CTACGGCGT 1681 G CAGTGCTTC A GCCGCTACC C CGACCACAT G AAGCAGCAC G ACTTCTTCA A GTCCGCCAT 1741 G CCCGAAGGC T ACGTCCAGG A GCGCACCAT C TTCTTCAAG G ACGACGGCA A CTACAAGAC 1801 C CGCGCCGAG G TGAAGTTCG A GGGCGACAC C CTGGTGAAC C GCATCGAGC T GAAGGGCAT 1861 C GACTTCAAG G AGGACGGCA A CATCCTGGG G CACAAGCTG G AGTACAACT A CAACAGCCA 1921 C AACGTCTAT A TCATGGCCG A CAAGCAGAA G AACGGCATC A AGGTGAACT T CAAGATCCG 1981 C CACAACATC G AGGACGGCA G CGTGCAGCT C GCCGACCAC T ACCAGCAGA A CACCCCCAT 2041 C GGCGACGGC C CCGTGCTGC T GCCCGACAA C CACTACCTG A GCACCCAGT C CGCCCTGAG 2101 C AAAGACCCC A ACGAGAAGC G CGATCACAT G GTCCTGCTG G AGTTCGTGA C CGCCGCCGG 2161 G ATCACTCTC G GCATGGACG A GCTGTACAA G TAGGAATTC G ATATCAAGC T TATCGATAA 2221 T CAACCTCTG G ATTACAAAA T TTGTGAAAG A TTGACTGGT A TTCTTAACT A TGTTGCTCC

2281 T TTTACGCTA T GTGGATACG C TGCTTTAAT G CCTTTGTAT C ATGCTATTG C TTCCCGTAT

2341 G GCTTTCATT T TCTCCTCCT T GTATAAATC C TGGTTGCTG T CTCTTTATG A GGAGTTGTG