盐酸多西环素 2010版中国药典分析

Nov.15,2011

LC Application Lab,Shiseido China

No.31,BDA International Business Park,

2Jing Yuan Street,Beijing

Economic Technological Development Zone,

Beijing,China 100176

Phone 010-6785-6801,FAX 010-6785-6882

盐酸多西环素2010版药典分析

Fig.1

Fig.2

HPLC Conditions

Column:CAPCELL PAK MGII 5μm,4.6mm i.d.×250mm (Col.No.A4AD03965)

Oven temp.:35°C

M.phase:A)0.25mol/L CH3COONH4:0.1mol/L EDTA:TEA=100:10:1,B)CH3CN,A:B=85:15Flow rate:1000μL /min

Inj.vol.:20μL

Sample:0.2mg/mL,dissolved in 0.01mol/L HCl

Detector:PDA 280nm

盐酸多西环素容易和填料表面重金属离子螯合，导致色谱峰拖尾及分离度变差。通过采用了聚合物包被处理的资生堂CAPCELL PAK MGII 色谱柱，按照2010版药典方法成功保留了盐酸多西环素，并且色谱峰形良好，重现性好，主峰与其后杂质峰得到良好分离（分离度2.31，见Fig.1-3）。

郭彦丽城田修盐酸多西环素

Doxycycline Hyclate