IGF-1ELISA试剂盒说明书,大鼠胰岛素样生长因子1

IGF-1ELISA试剂盒说明书，大鼠胰岛素样生长因子1

IGF-1ELISA试剂盒说明书，大鼠胰岛素样生长因子1

Elisa kit规格：48孔配置/96孔配置

标准品稀释液：1.5ml×1瓶

酶标试剂：3 ml×1瓶（48）/6 ml×1瓶（96）

用途：科研实验等领域科学研究，不得用于临床诊断。

供应商：上海樊克生物有限公司

友情提醒：公司经营的产品均为科研实验用，不可用于临床应用。

IGF-1ELISA试剂盒说明书，大鼠胰岛素样生长因子1Elisa kit test rules:

1, to ensure the accuracy of the gun, the error can not be more than 2%. Available water and electronic balances are determined. But it's better to have professional personnel to correct it.

2, to be equipped with 20ul, 50ul, 100ul, 1000ul and a volley. Draw different liquids, to replace the gun head. Even when the standard is drawn.

1, 3 hours before the experiment to remove the kit from the refrigerator, so that a variety of reagents are restored to room temperature, in order to make the results more stable.

4, the experiment, to make the substrate to avoid light storage.

5, with the gun to draw the liquid speed can not be too fast, so as not to produce bubbles and to absorb the amount is not accurate.

6, when the liquid, to use the range and the need to close the gun to suck, reduce the error.

7, when the liquid is added to the enzyme standard hole, the liquid contact of the liquid drop and the hole wall of the gun head can be avoided by avoiding the contact of the liquid drop and the hole wall in the gun head.

8, after all the liquid added, the enzyme labeled plate on the table in parallel to gently shake 30 seconds, mixed with liquid. Can also use the shaking function of the enzyme standard instrument. 9, should try to do two experiments, so as to ensure the accuracy of the data. 10, the results have questions about the sample to be confirmed by other methods.

IGF-1ELISA试剂盒说明书，大鼠胰岛素样生长因子1Operation steps

标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在第一、第二孔中分别加标准品100μl?，然后在第一、第二孔中加标准品稀释液50μl，混匀；然后从第一孔、第二孔中各取100μl分别加到?第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各?取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul?，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标?准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在 Tenth ninth holes, respectively, plus standard product diluted solution 50 L, after mixing from the tenth hole in the 50 to take ninth Mu L abandoned. The samples were diluted with 50 L, and the concentrations were 1800ng/L, 1200ng/L, 600ng/L,, 300ng/L, and 150ng/L, resp

Add sample: respectively, set the blank hole (blank control hole without samples and enzyme labeled reagents, and the rest of the steps are the same), to be measured sample hole. In the? ELISA coated plate to be tested on a sample hole Zhongxian sample dilution of 40 g l, then add to be measured 10 mu l of sample (sample final dilution degrees for 5 times)?. The sample is added to the bottom of the enzyme labeled plate hole, as far as possible without touching the wall of the hole.

Temperature and education: 30 minutes after the closure of the plate with a sealing plate, 37.