玉米品质鉴定一致性检测40 对核心引物序列(基础20对加拓展20对)科学版

序号

标记位点染色体位置重复序列片段大小

Serial

Locus Bin Motif

sequence

range

1

bnlg439w1 d1.03(TC)19 319-369

2

umc1335y5 c1.06(AG)24233-257

3

umc2007y4a2.04(TC)20 233-300

4

bnlg1940k7c2.08(CT)16 324-388 5umc2105k3

c

3(AG)17 280-350

6

phi053k2 d 3.05(GTAT)5333-363

7

phi072k4 b 4.01(TGTT)4 408-432 8bnlg2291k4

b

4.06(AG)16 362-421 9umc1705w1

b

5.03(CT)31 254-349 10bnlg2305k4

d

5.07(GA)21 240-312

11

bnlg161k8 b6(AG)22 154-216 12bnlg1702k1

b

6.05(CT)10 260-347 13umc1545y2

d

7(AAGA)4 180-249 14umc1125y3

b

7.04(CTCG)4 149-175

15

bnlg240k1 c8.06(GA)14220-239

16

phi080k15 c8.08(GGAGA)5 202-238

17

phi065k9 d9.03(GTGAA)3( GTGCA)3

391-415

18umc1492y1

3 c 9.04(GCA)

4 270-290

19

umc1432y6 c10.02(TC)6 211-259

20umc1506k1

2 a 10.05(TTTG)7 163-196

21umc1147y4

d

1.07(CA)7149-172

22bnlg1671y1

7 a 1.1(CT)21 173-255

23phi96100y1

a

2(AGGT)9 231-287

24umc1536k9

d 2.07

(GT)10(TA)

4

216-238

25bnlg1520K1

a 2.09

(CT)9(AC)8

(GA)4(TA)4

164-202

26umc1489y3

d

3.07(GCG)5231-265

27

bnlg490y4 d 4.04(TA)4245-331

28

umc1999y3 c4.09(TGC)9 167-200

29umc2115k3

b

5.02(GCCAT)5 265-295

30umc1429y7

b

5.03(AGC)4125-143

31

bnlg249k2 b 6.01(AG)10 259-313

32phi299852y

2 b 6.07(CTG)5200-254

33umc2160k3

b

7.01(AG)11198-244 34umc1936k4

c

7.03(TG)6153-176 35bnlg2235y5

b

8.02(TG)6174-198

36phi233376y

1 c 8.09(CCG)180-222

37umc2084w2

d

9.01(CTAG)5184-214

38umc1231k4

a

9.05(GA)10239-283

39

phi041y6 c10(CAGC)4 296-334 40umc2163w3

d

10.04(AG)28280-352

左引物序列右引物序列

Forward Primer sequence (5’-3’)Reverse Primer sequence (5’-3’) AGTTGACATCGCCATCTTGGTGAC GAACAAGCCCTTAGCGGGTTGTC CCTCGTTACGGTTACGCTGCTG GATGACCCCGCTTACTTCGTTTATG TTACACAACGCAACACGAGGC GCTATAGGCCGTAGCTTGGTAGACAC CGTTTAAGAACGGTTGATTGCATTCC GCCTTTATTTCTCCCTTGCTTGCC GAAGGGCAATGAATAGAGCCATGAG ATGGACTCTGTGCGACTTGTACCG CCCTGCCTCTCAGATTCAGAGATTG TAGGCTGGCTGGAAGTTTGTTGC GCTCGTCTCCTCCAGGTCAGG GAGCTTCTTTTGCACCACAAGGTC GCACACCCGTAGTAGCTGAGACTTG CATAACCTTGCCTCCCAAACCC GGAGGTCGTCAGATGGAGTTCG CACGTACGGCAATGCAGACAAG CCCCTCTTCCTCAGCACCTTG CGTCTTGTCTCCGTCCGTGTG TCTCAGCTCCTGCTTATTGCTTTCG GATGGATGGAGCATGAGCTTGC GATCCGCATTGTCAAATGACCAC AGGACACGCCATCGTCATCA AATGCCGTTATCATGCGATGC GCTTGCTGCTTCTTGAATTGCGT GGATGATGGCGAGGATGATGTC CCACCAACCCATACCCATACCAG GCAGGTGTCGGGGATTTTCTC GGAACTGAAGAACAGAAGGCATTGATAC TGAACCACCCGATGCAACTTG TTGATGGGCACGATCTCGTAGTC CGCCTTCAAGAATATCCTTGTGCC GGACCCAGACCAGGTTCCACC GCGGAAGAGTAGTCGTAGGGCTAGTGTAG AACCAAGTTCTTCAGACGCTTCAGG GAGAAATCAAGAGGTGCGAGCATC GGCCATGATACAGCAAGAAATGATAAGC GAGGAATGATGTCCGCGAAGAAG TTCAGTCGAGCGCCCAACAC AAGAACAGGACTACATGAGGTGCGATAC GTTTCCTATGGTACAGTTCTCCCTCGC CCCGACACCTGAGTTGACCTG CTGGAGGGTGAAACAAGAGCAATG TTTTGCACGAGCCATCGTATAACG CCATCTGCTGATCCGAATACCC TGATAGGTAGTTAGCATATCCCTGGTATCG GAGCATAGAAAAAGTTGAGGTTAATATGGAGC CACTCTCCCTCTAAAATATCAGACAACACC GCTTCTGCTGCTGTTTTGTTCTTG GCTACCCGCAACCAAGAACTCTTC GCCTACTCTTGCCGTTTTACTCCTGT GGTGTTGGAGTCGCTGGGAAAG TTCTCAGCCAGTGCCAGCTCTTATTA GGCCACGTTATTGCTCATTTGC GCAACAACAAATGGGATCTCCG