法庭科学SWGDAM验证流程
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• SWGDAM Chairman is appointed by the FBI Director, and members are selected by the Chairman from forensic DNA laboratories.
• SWGDAM has multiple committees including Autosomal STR Committee, CODIS Committee, Rapid DNA Committee, Next Generation Sequencing Working Group, etc.
• TWGDAM continued to provide a level of direction to the forensic DNA community by issuing guidelines for DNA analysis, and the guidelines became de facto standards and were recognized by courts.
Size deviation of 42 population samples showed deviation <0.50bp for all alleles
遗传标记在不同种族的人群中的分布
Allele for
D6S1043 Chinese
• All sample types have ≥90% pass rate (PAT = 175 rfu) with 1 Proportion test. • The recommended rxn volume for HXP is 25ul, some tests here were done
• Low peaks were detected with bovine, dog (like contamination, also found in the repeat), horse, mouse and pig.
华夏白金一次性成功通过率
SWGDAM 3.4 The ability to obtain results from DNA recovered from biological samples deposited on various substrates and subjected to various environmental and chemical insults should be evaluated.
法庭科学SWGDAM验证流程
The world leader in serving science
• SWGDAM 介绍
议程
• 华夏TM白金SWGDAM 验证
• YfilerPlusTM SWGDAM 验证
• ISO 18385 介绍
SWGDAM主页
Source: swgdam.org
关于SWGDAM
SWGDAM法医DNA分析方法验证指导
3.1 Genetic Markers
3.6 Case Type Samples
3.2 Species Specificity 3.3 Sensitivity
3.7 Population Studies 3.8 Mixture Studies
3.4 Stability
华夏白金物种特异性 – 非灵长类 SWGDAM 3.2 The ability to detect genetic information from non-targeted species (e.g.,
Bovin e
detection of microbial DNA in a human assay) should be determined.
Chicke n
Do g
700 r来自百度文库u
Pi g
200
Rabb it
rfu
Hors
Ra
e
t
Microb es
Shee p
Mous
NT
e
C
• 10 ng non-primate DNA, or 105 copies of microbes (species commonly found in the oral cavity, listed in User Guide) were added to the system using 29 PCR cycles.
• First meeting of Technical Working Group on DNA Analysis Methods (TWGDAM) was held in November 1988, with a subcommittee on restriction fragment length polymorphism (RFLP) DNA analysis.
with 10ul reaction volume to challenge the system.
样本准在3500xl上的准确度
SWGDAM 3.5 Precision and accuracy of the assay should be demonstrated. Accuracy is the degree of conformity of a measured quantity to its actual (true) value.
• One of SWGDAM’s most important responsibilities is the recommendation of revisions to the FBI’s Quality Assurance Standards (QAS) for DNA Analysis.
SWGDAM 出版物
3.9 PCR Based Studies
3.5. Precision & Accuracy
SWGDAM Validation Guidelines for Forensic DNA Analysis Methods approved in November 2012 www.swgdam.org
200 rfu
• SWGDAM has multiple committees including Autosomal STR Committee, CODIS Committee, Rapid DNA Committee, Next Generation Sequencing Working Group, etc.
• TWGDAM continued to provide a level of direction to the forensic DNA community by issuing guidelines for DNA analysis, and the guidelines became de facto standards and were recognized by courts.
Size deviation of 42 population samples showed deviation <0.50bp for all alleles
遗传标记在不同种族的人群中的分布
Allele for
D6S1043 Chinese
• All sample types have ≥90% pass rate (PAT = 175 rfu) with 1 Proportion test. • The recommended rxn volume for HXP is 25ul, some tests here were done
• Low peaks were detected with bovine, dog (like contamination, also found in the repeat), horse, mouse and pig.
华夏白金一次性成功通过率
SWGDAM 3.4 The ability to obtain results from DNA recovered from biological samples deposited on various substrates and subjected to various environmental and chemical insults should be evaluated.
法庭科学SWGDAM验证流程
The world leader in serving science
• SWGDAM 介绍
议程
• 华夏TM白金SWGDAM 验证
• YfilerPlusTM SWGDAM 验证
• ISO 18385 介绍
SWGDAM主页
Source: swgdam.org
关于SWGDAM
SWGDAM法医DNA分析方法验证指导
3.1 Genetic Markers
3.6 Case Type Samples
3.2 Species Specificity 3.3 Sensitivity
3.7 Population Studies 3.8 Mixture Studies
3.4 Stability
华夏白金物种特异性 – 非灵长类 SWGDAM 3.2 The ability to detect genetic information from non-targeted species (e.g.,
Bovin e
detection of microbial DNA in a human assay) should be determined.
Chicke n
Do g
700 r来自百度文库u
Pi g
200
Rabb it
rfu
Hors
Ra
e
t
Microb es
Shee p
Mous
NT
e
C
• 10 ng non-primate DNA, or 105 copies of microbes (species commonly found in the oral cavity, listed in User Guide) were added to the system using 29 PCR cycles.
• First meeting of Technical Working Group on DNA Analysis Methods (TWGDAM) was held in November 1988, with a subcommittee on restriction fragment length polymorphism (RFLP) DNA analysis.
with 10ul reaction volume to challenge the system.
样本准在3500xl上的准确度
SWGDAM 3.5 Precision and accuracy of the assay should be demonstrated. Accuracy is the degree of conformity of a measured quantity to its actual (true) value.
• One of SWGDAM’s most important responsibilities is the recommendation of revisions to the FBI’s Quality Assurance Standards (QAS) for DNA Analysis.
SWGDAM 出版物
3.9 PCR Based Studies
3.5. Precision & Accuracy
SWGDAM Validation Guidelines for Forensic DNA Analysis Methods approved in November 2012 www.swgdam.org
200 rfu