测序通用引物名称及序列表
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测序通用引物名称及序列列表
载体名称上游引物（5'primer）下游引物（3'primer）
P3*FLAG-CMV CMV-30(825-854)CMV-24(1129-1152) pAc5.1/V5-His(_A,_B,_C)pAc5-5BGH rev pAcG2T pGEX-5not available
pACT T7EEV T3/EBVrev
pACT2GAL4ADfor pGAL4-ADrv
pACT2p17110p12584 pACYC184(BamHI-site)PBRforBam PBRrevBam
pACYCDuet-1(MCSI)ACYCDuetUP1Primer DuetDOWN1 pACYCDuet-1(MCSII)DuetUp2T7-Terminator pAdEasy-1not available not available
pAdTrack-CMV not available not available pAS2-1GAL4Bdfor/P24990pGAL4-BDrv/P31430
pB42AD pB42ADF PB42ADR
pBABE pBMN_5'
pBacPAK8BAC1BAC2
pBAD pBAD-F pBAD-R pBAD/HisABC pBAD-5'
pBBR1MCS T3/M13rev T7/M13for
pBD-GAL4pBD-GAL4-F PBD-GAL4-R
pBI12135S NOS
pBIND GAL4-Bdfor T3,EBVrev
pBK-CMV M13F/T7M13R/T3 pBluescriptIIKS(+/-)T3/M13rev T7/M13for（优先用M13F）pBluescriptIISK(+/-)T3/M13rev T7/M13for
pBluescriptKS(+/-)T3/M13rev T7/M13for
pBluescriptSK(+/-)T3/M13rev T7/M13for
pBR322(BamHI-site)PBRforBam PBRrevBam
pBR322(EcoRI/HindIII-sites)pBRforEco pBRrevHind pBR325(BamHI-site)PBRforBam PBRrevBam
pBR329(BamHI-site)PBRforBam PBRrevBam
pBridge(MCSI)GAL4-Bdfor3'BD
pBridge(MCSII)not available not available pBS-T II T3/M13rev T7/M13for
pBS（AMP）T7T3 pBSR T3T7ter pBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrev
pBudCE4.1(MCSII)EF-1αFwd BGH
pBudCE4.1/lacZ/CAT CMV-Profor,T7c-mycrev,EBVrev pBV220pBV220F PBV220R
pCAL-c T7/Tac-Profor T7-Terminator
pCAL-n T7/Tac-Profor T7-Terminator pCAMBIA1301(1300)pCAMBIA1301-5'(ECORI)/M13R pCAMBIA1301-3'(HindIII) pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII) pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49)
pCANTB5E S1S6
pCAT3-enhancer RVP3此载体无反向引物pCDFDuet-1(MCSI)ACYCDuetUP1Primer DuetDOWN1
pCDFDuet-1(MCSII)DuetUP2T7TER pcDNA1.1CMV-Profor,T7pCDM8-3
pcDNA2.1M13for/T7M13rev/Tac-Profor
pcDNA3T7/CMV-Profor BGH rev/SP6 pcDNA3.1(+/-)pcDNA3.1F/T7/CMV-Profor BGH rev pcDNA3.1/His(_A,_B,_C)T7/CMV-Profor BGH rev pcDNA3.1/myc-His(_A,_B,_C)T7/CMV-Profor BGH rev/c-mycrev pcDNA3.1/V5-His(_A,_B,_C)T7/CMV-Profor BGH rev/V5rev pcDNA3.1/Hygro(+,-)T7/CMV-Profor BGH rev/V5rev
pcDNA3.1/Zeo(+.-)T7BGHrev
pcDNA3.3TOPOTA CMV-Profor TKPAreverse pcDNA4/HisMax(_A,_B,_C)Xpressfor BGH rev
pcDNA4/HisMax-TOPO Xpressfor BGH rev pcDNA4/HisMax-TOPO/lacZ Xpressfor BGH rev pcDNA4/myc-His(_A,_B,_C)T7,CMV-Profor BGH rev,c-mycrev pcDNA4/TO TREfor/CMV-Profor BGH rev pcDNA4/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5rev pcDNA4-TET/ON/AMP+CMV-Profor
pcDNA5/FRT T7/CMV-Profor BGH rev pcDNA6/myc-His(_A,_B,_C)CMV-Profor,T7BGH rev,c-mycrev pcDNA6/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5rev pCEP4pCEP-F/CMV-Profor EBVrev
pCI T7（17BASE）EBVrev
pCI-neo T7（17BASE）EBVrev,T3
pCMS-EGFP T7T3
pCMS-EGFP T7EBVrev,T3
pCMV-3Tag-4A T3/PFLAG-CMV-F T7 pCMV5pCMV5F PCMV5R
pCMV6CMV-Profor pCMV6-3、HGHrev
pCMV6-XL4CMV-Profor/T7pCMV6-3、HGHrev
pCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrev pCMV-MYC(AMP+)PCMV-F PCMV-R,EBVrev